Effect and mechanism of Heshowuyin on alleviating the hippocampal neurons injury induced by β-amyloid protein

doi:10.16098/j.issn.0529-1356.2015.02.006

Abstract

Abstract:

Objective To investigate the protective effect of Heshouwuyin on primary hippocampal neuronal cells induced by β-amyloid protein（Aβ）and its possible mechanisms. Methods Hippocampal neurons collected were from newborn SD rats aged in 24 hours.Hippocampal cells which were cultured 10 days were divided into five groups:Model group,Heshouwuyin prevention group,Heshouwuyin treatment group,Heshouwuyin control group and Normal control group.Hoechst33258 staining was used to observe the apoptosis rate of the hippocampal cells.Immunohistochemistry staining was used to observe the expression of Trx1.The mRNA expression of Trx1 was detected in the hippocampal neuronal cells of each group with reverse transcription polymerase chain reaction , and the protein expression of Trx1 was detected in the hippocampal neuronal cells with Western blotting. Results The apoptosis rate of hippocampal neuronal cells was significantly increased in the Model group (P<0.05).In the Model group,the expresstion of Trx1 was significantly decreased(P<0.05).Compared with the Model group,the apoptosis rate of hippocampal neuronal cells was significantly decreased in the Heshouwuyin prevention group and Heshouwuyin treatment group(P<0.05).The mRNA and protein expresstions of Trx1 were significantly higher in Heshouwuyin prevention group and Heshouwuyin treatment group than that in the Model group(P<0.05).Conclusion Heshouwuyin can significantly alleviate the Hippocampal neurons injury induced by Aβ. The neuroprotective activities of Heshouwuyin can be related to its ability to increase the Trx1 expression level.

Key words: Hippocampal neuron, Alzheimer’s disease, β-amyloid protein, , Heshouwuyin, , Thioredoxin, Immunohistochemistry, Rat

CHEN Jing-bo WANG Yu-juan GUO Sheng-nan NIU Si-yun* DUAN Fei*. Effect and mechanism of Heshowuyin on alleviating the hippocampal neurons injury induced by β-amyloid protein[J]. AAS, 2015, 46(2): 175-181.

References

［1］Bush AI. Drug development based on the metals hypothesis of Alzheimer’s disease［J］.J Alzheimers Dis, 2008, 15(2):223240.
［2］Liu J, Qiu Y, He J, et al. Effect of ginsenoside Rb1 and Rg1 on hippocampal neurous.［J］.Journal of Tongji University(Medical Science),2011,32(3):1-6.(in Chinese)
刘娟，裘莹，何晶，等.人参皂苷Ｒｂ１和Ｒｇ１对海马神经元的影响［J］.同济大学学报(医学版)，2011，32（3）：1-6.
［3］Smitha MA，Zhua XW，Tabatonb M，et al.Increased iron and free radical generation in preclinical Alzheimer disease and mild cognitive impairment［J］.J Alzheimer’s Dis,2010,19(1): 363-372.
［4］Nishida Y, Ito S, Ohtsuki S, et al. Depletion of vitamin E increases abeta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer disease［J］.J Biol Chem, 2009, 284 (48) : 33400-33408.
［5］Blecharz-Klin K, Piechal A, Joniec I, et al. Pharmacological and biochemical effects of Ginkgo biloba extract on learning, memory consolidation and motor activity in old rats［J］.Acta Neurobiol Exp (Wars) , 2009, 69 (2) :217-231.
［6］Luo Y, Nie J, Gong QH, et al. Protective effects of icariin against learning and memory deficits induced by aluminium in rats［J］. Clin Exp Pharmacol Physiol, 2007, 34(8): 792-795
［7］Wang XK, Wang R, Yuan Zhf. Study on effect of polygonum mutiflorum extract on lipid metabolism and its anti-oxidation in SD rats with hyperlipemia［J］.China Pharmaceuticals,2009,18(24):19-20.(in Chinese)
相聪坤，王蕊，袁志芳.何首乌二苯乙烯苷类提取物对高脂血症大鼠血脂代谢的影响及其抗氧化作用［J］.中国药业，2009，18（24）：19-20.
［8］Pang M, Li XL, Lu G, et al. Antioxidant and antifungal activities of ethanol extract from salvia miltior rhiza fibrous roots［J］.Journal of Northwest Forestry University, 2011,26(4):196-201.（in Chinese)
庞明，李西柳，陆刚，等.丹参须根乙醇提取物抗氧化及抑真菌活性研究［J］.西北林学院学报，2011，26(4)：196-201.
［9］Li YL, Chu W, Guo WCh, et al. The effect of Heshouwu decoction on anti-oxidation and blood lipid of aging rats［J］.Chinese Journal of Gerontology,2008,28(6):525-526.（in Chinese)
李亚丽，楚伟，郭文潮，等.何首乌饮对衰老大鼠抗氧化能力及血脂的影响［J］.中国老年学杂志，2008，28（6）：525-526.
［10］Zhang N, Li YL, Niu SY,et al. Study on anti-aging in the ovary of the aging model rat by traditional Chinese medicine Heshouwuyin［J］.Acta Anatomica Sinica，2008,39(2):187-192.(in Chinese)
张娜, 李亚丽,牛嗣云,等。中药何首乌饮抗大鼠卵巢组织衰老的机理［J］.解剖学报。2008，39（2）：187-192.
［11］Niu S, Chen J, Duan F,et al. Possible mechanism underlying the effect of Heshouwuyin, a tonifying kidney herb, on sperm quality in aging rats［J］.BMC Complement Altern Med, 2014 ,14: 250.
［12］Brouard A, Pelapart D, Dana C, et al. Mesencephalic dopaminergic neurons in primary cultures express functional neurotensin receptors［J］.J Neurosci,1992,12(4):1409-1415.
［13］Huang ChH，Lu Y，Gao XJ, et al.Advances of serum pharmacology of Chinese medicine［J］. Chinese Journal of Experimental Traditional Medical Formulae，2011,17(10):266-271.(in Chinese)
黄臣虎,陆茵,高骁君,等.中药血清药理学研究进展［J］.中国实验方剂学杂志，2011，17（10）：266-271.
［14］Xu X, Sun W, Sheng L, et al. Gene expression of KIFs in cortex of rats injected with β-amyloid into brain ventrical［J］.Acta Anatomica Sinica, 2006, 37(1):40-42.（in Chinese）
徐心, 孙伟, 盛莉,等. 脑室内注射β淀粉样蛋白造成大鼠大脑皮层KIF家族基因表达变化［J］. 解剖学报, 2006，37（1）：40-42.〖ZK)〗
［15］Aliev G，Obrenovich ME，Reddy VP, et al. Antioxidant therapy in Alzheimer’s disease: theory and practice［J］.Mini Rev Med Chem,2008,8(13):1395-1406.
［16］Kim MJ，Lee J，Seong AR，et al. Neuroprotective effects of Eriobotrya japonica against β-amyloid-induced oxidativen stress and memory impairment［J］.Food Chem Toxicol,2011,49(4):780-784.
［17］Takagi Y, Hattori I, Nozaki K, et al. Excitotoxic hippocampal injury is attenuated in thioredoxin transgenic mice［J］. J Cereb Blood Flow Metab, 2000, 20(5): 829-833.
［18］Chen B, Guan D, Cui J, et al. Thioredoxin1 down regulates MCP-1 secretion and expression in human endothelial cells by suppressing nuclear Translocation of activator protein 1 and redox factor-1［J］. Am J Physiol cell Physiol, 2010, 298(5): C1170-1179.
［19］Luo RZh, Jia W, Zhao LB, et al. General review on Radix Plygoni Multiflori［J］.Chinese Traditional and Herbal Drugs，2005,36（7）:1097-1100.(in Chinese)
罗瑞芝, 贾伟, 赵利斌,等.何首乌研究进展［J］. 中草药，2005，36（7）：1097-1100.
［20］Wang Z, Zhang X, Wang H, et al.Neuroprotective effects of icaritin against beta amyloid-induced neurotoxicity in primary cultured rat neuronal cells via estrogen-dependent pathway［J］. Neuroscience, 2007 ,145(3):911-922.
［21］Zhang ZY, Li C, Zug C,et al.Icariin ameliorates neuropathological changes, TGF-β1 accumulation and behavioral deficits in a mouse model of cerebral amyloidosis［J］.PLoS One, 2014,9(8):e104616.
［22］Yu H, Yao L, Zhou H，et al. Neuroprotection against Aβ25-35-induced apoptosis by Salvia miltiorrhiza extract in SH-SY5Y cells［J］.Neurochem Int, 2014,75:89-95.
［23］Jiang P, Li C, Xiang Z, et al. Tanshinone IIA reduces the risk of Alzheimer’s disease by inhibiting iNOS, MMP-2 and NF-κBp65 transcription and translation in the temporal lobes of rat models of Alzheimer’s disease［J］.Mol Med Rep, 2014,10(2):689-694.

[1]	FENG Li-ping XU Yi-lin CHEN Xun LIU Da-hai WANG Jun-yong WANG Xiao-ying LIN Jin-xing. Retinal microstructure and developmental characteristics in Zebrafish [J]. Acta Anatomica Sinica, 2024, 55(1): 105-112.
[2]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[3]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[4]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[5]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[6]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[7]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[8]	YANG Yang SHI Jun LI Kun MA Yuan ZHANG Shao-jie HOU Er-fei WANG Chao-qun CHEN Jie WANG Xing LI Zhi-jun. Finite element analysis of cervical intervertebral discs after removing different ranges of uncinate processes [J]. Acta Anatomica Sinica, 2024, 55(1): 88-97.
[9]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[10]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[11]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[12]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[13]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[14]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.
[15]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.