AAS ›› 2015, Vol. 46 ›› Issue (6): 772-779.doi: 10.16098/j.issn.0529-1356.2015.06.008

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Functions of Wnt-10a during K562 cell differentiation induced by hemin

WANG Jie HU Jiang-jiang ZHAO Fu-kun ZHANG Shi-fu*   

  1. School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
  • Received:2015-04-17 Revised:2015-06-21 Online:2015-12-06 Published:2015-12-06
  • Contact: ZHANG Shi-fu E-mail:cklzhang@163.com

Abstract:

Objective To study expression changes and functions of Wnt-10a protein in K562 differentiation induced by hemin. Methods Benzidine staining was used to test hemininduced erythroid differentiation of K562 cells. Western blotting and immunocytochemistry were used to detect protein expression changes of Wnt-10a and protein localization in K562 cells. Real-time PCR and Real-time PCR were applied to investigate mRNA expression of key proteins in Wnt signalling pathway during K562 defferentiation process. Wnt signaling pathway activator and Wnt-10a high expression K562 cell line were used to explore the influence of altered Wnt pathway. Results Wnt-10a protein and mRNA expressions were upregulated after a brief down in the erythroid differentiation process of K562 cells induced by hemin when, Wnt-10a protein migrated to the cell membrane. The key factors of the main Wnt signaling pathways changed during K562 differentiation. By using Wnt signaling pathway inhibitor, cell proliferation capability was enhanced during K562 differentiation by hemin. When Wnt-10a was highly expressed, the ability of induced differentiation of K562 cells was improved. Conclusion These results show that -10a protein is closely related with K562 cell differentiation induced by hemin, which may offer clues for further study of the mechanism of leukemia and cell differentiation.

Key words: Wnt-10a, K562, Hemin, Erythroid, Differentiation, Western blotting, Human