Abstract:

Objective To explore the effect of cod skin oligopeptide(CSO) on the proliferation in human gastric carcinoma SGC-7901 cells. Methods After treatment of SGC-7901 cells with different concentrations of CSO, the cells were observed under the microscope and the viability of cells was detected by CCK-8 assay. Cell apoptosis and cell cycle were determined by Western blotting and flow cytometry. Results CSO significantly suppressed the proliferation of SGC-7901 cells in a time-and dose-dependent manner（P<0.05）. The cultured SGC-7901 cells were treated by CSO for 24 hours and 48 hours and 72 hours with the IC50of 156.90 g/L and 102.10 g/L and 73.13 g/L.A large number of nuclear fragmentation and apoptotic bodies were observed under fluorescence microscope after treatment by DAPI. After treatment with CSO for 24 hours, SGC-7901 cells showed increased at G₁ stage and cell cycle arrested at S/G₂ stage, and the cell apoptosis rate increased with the CSO. With the increase of CSO concentration, the expression of Caspase-3 and Caspase-9 was up-regulated, and the expression of Bcl-2 was down-regulated. Conclusion CSO can promote the apoptosis and inhibit the proliferation of SGC-7901 cells. Anti-cancer mechanism may be related with Caspase-3 and Caspase-9 up-regulating,and Bcl-2 was down-regulating.

Key words: Cod skin oligopeptide, Gastric cancer, SGC-7901, Caspase-3, Caspase-9, Bcl-2, Western blotting, Flow cytometry, Human