Expression of ephrin A3 during valproate induced neural stem cells differentiation

doi:10.16098/j.issn.0529-1356.2018.03.004

Abstract

Abstract:

Objective To investigate the expression of ephrin A3 (Efna3) in the process of neural stem cells differentiation induced by valproate (VPA) and the expression pattern of Efna3 in the nervous system. Methods The hippocampal neural stem cells of SD rats were isolated and cultured. At 24 hours and 48 hours after VPA inducement, the dynamic expression of Efna3 was detected by Real-time PCR, Western blotting and immunofluoresence assay. Real-time PCR was used to detect the expression of Efna3 in various tissues, as well as neural stem cells, astrocytes and neurons of adult SD rats. Results The expression of Efna3 increased significantly in the VPA group compared to the control group. Efna3 showed dominant expression in the cerebrum and hippocampus, and its expression was higher in neurons and lower in astrocytes. Conclusion The promotion of VPA to the differentiation of neural stem cells may be related to the upregulation of Efna3.

Key words: Ephrin A3, Valproate, Neural stem cell, Neuron, Real-time PCR, Rat

LI Wen HE Hui PENG Min QIN Jian-bing SHI Jin-hong JIN Guo-hua. Expression of ephrin A3 during valproate induced neural stem cells differentiation[J]. Acta Anatomica Sinica, 2018, 49(3): 294-298.

References

［1］Perry EK, Johnson M, Ekonomou A, et al. Neurogenic abnormalities in Alzheimer’s disease differ between stages of neurogenesis and are partly related to cholinergic pathology ［J］. Neurobiol Dis, 2012, 47(2):155-162.

［2］Palmer TD, Takahashi J, Gage FH. The adult rat hippocampus contains primordial neural stem cells ［J］. Mol Cell Neurosci, 1997, 8(6):389-404.

［3］Kandasamy M, Roll L, Langenstroth D, et al. Glyconjugates reveal diversity of human neural stem cells (hNSCs) derived from human induced pluripotent stem cells (hiPSCs) ［J］. Cell Tissue Res, 2017, 368(3):531-549.

［4］Li HM, Zhu PP, Jin GH, et al. Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration ［J］.Acta Anatomica Sinica, 2014, 45(4):441-445. (in Chinese)

李浩明, 朱培培, 金国华, 等. 神经生长因子促进海马胆碱能神经再生过程中Lhx8的动态表达［J］. 解剖学报, 2014, 45(4):441-445.

［5］Tian ML, Zhu PP, Jin GH, et al. Lhx8 promotes the differentiation of hippocampal neural stem cells into cholinergic neurons in vitro ［J］.Acta Anatomica Sinica, 2013, 44(6):735-739. (in Chinese)

田美玲, 朱培培, 金国华, 等. Lhx8促进海马神经干细胞向胆碱能神经元分化［J］. 解剖学报, 2013, 44(6):735-739.

［6］Chu T, Zhou H, Wang T, et al. In vitro characteristics of valproic acid and all-trans-retinoic acid and their combined use in promoting neuronal differentiation while suppressing astrocytic differentiation in neural stem cells ［J］. Brain Res, 2015, 1596:31-47.

［7］Duchen MR. Mitochondria, calcium-dependent neuronal death and neurodegenerative disease ［J］. Pflugers Arch, 2012, 464(1):111-121.

［8］Huang Y, Mucke L. Alzheimer mechanisms and therapeutic strategies ［J］. Cell, 2012, 148 (6):1204-1222.

［9］Li XY, Bao XJ, Wang RZ, et al. Potential of neural stem cell-based therapies for Alzheimer’s disease ［J］. J Neurosci Res, 2015, 93(9):1313-1324.

［10］Swaminathan A, Kumar M, Halder Sinha S, et al. Modulation of neurogenesis by targeting epigenetic enzymes using small molecules: an overview ［J］. ACS Chem Neurosci, 2014, 5(12):1164-1177.

［11］Foley AG, Gallagher HC, Murphy KJ, et al. Pentyl-4-yn-valproic acid reverses age-associated memory impairment in the Wistar rat ［J］. Neurobiol Aging, 2004, 25(4):539-546.［12］Chu W, Yuan J, Huang L, et al. Valproic acid arrests proliferation but promotes neuronal differentiation of adult spinal nspcs from SCI rats ［J］. Neurochem Res, 2015, 40(7):1472-1486.

［13］Meganathan K, Jagtap S, Srinivasan SP, et al. Neuronal developmental gene and miRNA signatures induced by histone deacetylase inhibitors in human embryonic stem cells ［J］. Cell Death Dis, 2015, 6:e1756.

［14］Yu IT, Park JY, Kim SH, et al. Valproic acid promotes neuronal differentiation by induction of proneural factors in association with H4 acetylation ［J］. Neuropharmacology, 2009, 56(2):473-480.

［15］Hsieh J, Nakashima K, Kuwabara T, et al. Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells ［J］. Proc Natl Acad Sci USA, 2004, 101(47):16659-16664.

［16］Kania A, Klein R. Mechanisms of ephrin-Eph signalling in development, physiology and disease ［J］. Nat Rev Mol Cell Biol, 2016, 17(4):240-256.

［17］Katoh Y, Katoh M. Comparative integromics on Ephrin family ［J］. Oncol Rep, 2006, 15(5):1391-1395.

［18］Wang T, Chen J, Tang CX, et al. Inverse expression levels of EphrinA3 and EphrinA5 contribute to dopaminergic differentiation of human SH-SY5Y cells ［J］. J Mol Neurosci, 2016, 59(4):483-492.

［19］Carmona MA, Murai KK, Wang L, et al. Glial ephrin-A3 regulates hippocampal dendritic spine morphology and glutamate transport ［J］. Proc Natl Acad Sci USA, 2009, 106(30): 12524-12529.

［20］Murai KK, Nguyen LN, Irie F, et al. Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling ［J］. Nat Neurosci, 2003, 6(2):153-160.

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