Inhibitory effect of dihydromyricetin combined with etoposide on the choriocarcinoma cell JAR

doi:10.16098/j.issn.0529-1356.2018.03.010

Abstract

Abstract:

Objective To investigate the inhibitory effect and related mechanism of dihydromyricetin(DMY) combined with etoposide(VP16) on the choriocarcinoma cell JAR.Methods JAR cells cultured in vitro were devided into control group, DMY group, VP16 group and DMY+VP16 group. The cell proliferation was measured by MTT assay. The apoptosis rate of each group was analyzed by annexin V-FITC/PI double dye flow cytometry. The cell viability was measured by the colony formation capability assay. The protein expression levels of Bcl-2, inhibitor apoptosis protein 2(c-IAP2) were detected by Western blotting analysis. Results The result of MTT assay showed that compared with the control group, the cell survival ratio of DMY group, VP16 group and DMY+VP16 group reduced at 24 hours and 48 hours.The result of double dye flow cytometry showed that the apoptosis rate of the experimental groups were higher than that of the control group, and the apoptosis rate of DMY+VP16 group was significantly higher than those treated with DMY or VP16 alone.The result of the colony formation capability assay showed that,for JAR cells,the number of clones in experimental groups were less than that in the control group, and the number of clones in DMY+ VP16 group was the least. For normal liver HL7702 cells, there was no significant difference between DMY group and the control group, and there was no significant difference between VP16 group and DMY+VP16.The result of Western blotting showed that the protein expression levels of Bcl-2 and c-IAP2 in experimental group decreased compared with those in the control group, and the decrease of he protein expression levels of Bcl-2 and c-IAP2 in DMY+VP16 group were the most significant. Conclusion Dihydromyricetin combined with etoposide enhanced the inhibitory effect on choriocarcinoma cell JAR, which can reduce the dosage of the chemotherapy drug VP16.The inhibitory effect of tumor cells may be related to the down-regulation of protein expression of Bcl-2, C-IAP2.

Key words: Dihydromyricetin, Etoposide, JAR cell, Western blotting, Human

WANG Kang XU Qian ZUO Yan-zhen LIU Lei LU Yan-jie LEI Yun-tao CUI Ying LI Yu-hong. Inhibitory effect of dihydromyricetin combined with etoposide on the choriocarcinoma cell JAR[J]. Acta Anatomica Sinica, 2018, 49(3): 330-336.

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