TRIM28siRNA enhancing the sensitivity of non-small cell lung cancer cell PAa to etoposide through activating E2F transcription factor 1

doi:10.16098/j.issn.0529-1356.2018.04.007

Abstract

Abstract:

Objective To investigate the effect of small interfering RNA (siRNA)-mediated silencing of TRIM28（tripartite motif containing 28）on the sensitivity of non-small cell lung cancer cell (NSCLC) PAa to etoposide and explore the possible mechanism. Methods The expression of TRIM28 in PAa cell was silenced by RNA interference. MTT and colony formation assay were used to assess the inhibitory effect of TRIM28siRNA alone or in combination with etoposide on the growth and proliferation of PAa cells. The apoptosis of different groups was detected by flow cytometry(FCM ) and the expression of E2F transcription factor 1(E2F1) was evaluated by Western blotting. Results A PAa cell line silenced with the TRIM28siRNA was established. TRIM28 in combination with etoposide significantly inhibited the proliferation and colony formation of PAa cells. TRIM28siRNA in combination with etoposide induced apoptosis and increased the expression of E2F1 at mRNA and protein levels. Conclusion TRIM28siRNA in combination with etoposide has an obvious effect on the growth of NSCLC cells, suggesting a new clinical treatment strategy for NSCLC.

Key words: Non-small cell lung cancer, PAa cell, TRIM28, RNA interference, Etoposide, Western blotting, Human

LIU Lei GAO Ya-xian WANG Jian-ping ZHAO Xue-rong XU Qian HU Wang LIU Mei-qi. TRIM28siRNA enhancing the sensitivity of non-small cell lung cancer cell PAa to etoposide through activating E2F transcription factor 1[J]. Acta Anatomica Sinica, 2018, 49(4): 455-460.

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