Abstract:

Objective To investigate the effect of thawing and xenotransplantation of two kinds of cryopreservation method on human ovarian tissue by detecting the expression of angiopoietin-2 (Ang-2). Methods Sixteen cases of human ovarian tissue specimens were collected, and each case was divided into two parts. One part was cryopreserved by programmed freezing and the other one was cryopreserved by vitrification. Thirty-two SCID nude mice were divided into two groups: group A were transplantated the thawed human ovarian tissue frozen by programming freezing, and group B, by vitrification. After thawing and 6 weeks after transplantation, the survival rate of the primordial follicles and the expression of Ang-2 in the granulosa cells were observed in the two groups. Results After thawing, the positive rate of Ang-2 in group A was significantly higher than that in group B (P<0.05), and there was no significant difference in the normal follicular rate between the two groups (P>0.05). After transplantation, there were no significant differences in the survival rate of primordial follicles and the positive rate of Ang-2 between the two groups (P>0.05). There were no significant differences in the positive rate of Ang-2 (P>0.05) in two groups and there was no But in group B, the survival rate of primordial follicles after transplantation was lower than after thawing (P<0.05). Conclusion Programmed freezing had less effect on the expression of Ang-2 in the primordial follicle granulosa cells of the human ovarian tissue than vitrification. The heterograft of thawed human ovarian tissue does not affect the expression of Ang-2.

Key words: Ovarian tissue cryopreservation, Programmed freezing, Vitrification, Angiopoietin-2, Immunohistochemistry, Nude mouse