Effects of intrathecal injection of M_ERK2-shRNA adenovirus on diabetic neuropathic pain in mouse

doi:10.16098/j.issn.0529-1356.2019.05.004

Abstract

Abstract:

Objective To explore the mechanism of extracellular regulated protein kinase 2 (ERK2) in diabetic neuropathic pain(DNP) mice. Methods Forty mice were randomly divided into four groups: control group, DNP group, siERK2-DNP group (intrathecal injection of M_ERK2-shRNA adenovirus), siRNA-DNP group (intrathecal injection of siRNA adenovirus). The pain threshold was detected after the model made successfully, the mechanical pain threshold was detected before moulding, after moulding 2 weeks, 4 weeks and 6 weeks. Spinal cord tissues were taken after 6 weeks, and the expression of ERK2 and nuclear factor κB (NFκB) in the posterior horn of the spinal cord was detected by Western blotting and immunohistochemistry. Results Compared with the control group, the pain threshold of DNP group and siRNA-DNP group decreased significantly, the expression of ERK2 and NFκB increased significantly (P<0.05). Compared with the control group, the pain threshold of siERK2-DNP group had no significant change, and the expression of ERK2 and NFκB had no significant difference (P>0.05). Conclusion The marked down-regulation of ERK2 may be one of the important mechanisms affecting neuronal injury in the spinal dorsal horn of diabetic neuropathic pain.

Key words: siERK2, Diabetes, Neuropathic pain, Spinal dorsal horn neurons, Nuclear factor κB, Western blotting, Mouse

WANG Yue-jing YAO Hong-bo ZHANG Meng LIAN Jie ZHANG Hai-yan LIU Dan-yang LI Lin ZHANG Yu-hua LI Jin-he SUN Li-hui. Effects of intrathecal injection of M_ERK2-shRNA adenovirus on diabetic neuropathic pain in mouse[J]. Acta Anatomica Sinica, 2019, 50(5): 565-569.

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