Mucin 16 regulating activity and migration of gallbladder cancer cells through phosphoinositide 3-kinase/protein kinase B pathway

doi:10.16098/j.issn.0529-1356.2019.05.012

Abstract

Abstract:

Objective To investigate the effect of mucin 16(MUC16) on the viability and migration of gallbladder cancer cells (GBC-SD) through phospho inositide 3-kinase/protein kinase B (PI3k/Akt) pathway. Methods Firstly, after overexpression of MUC16, the matrix metalloproteinase(MMP) family proteins were screened by Real-time PCR. Secondly, after overexpression of MUC16, knockdown of MUC16 and PI3K/Akt pathway inhibitor BKM120, the protein levels of PI3K/Akt pathway and MMP-9 were detected by Western blotting. Finally, the proliferation, viability and migration of gallbladder cancer cells were detected by cell counting kit-8 (CCK-8) and scratch test. Results After overexpression of MUC16, the relative expression of mRNA in MMP-9 increased significantly (P<0.05). After overexpression of MUC16, the levels of MMP-9, p-Akt and PI3K increased significantly (P<0.05), but PI3K inhibitor BKM120 could avoid this phenomenon. After knocking down MUC16, the protein levels of MMP-9, p-Akt and PI3K decreased significantly (P<0.05).After overexpression of MUC16, the cell viability and migration ability of GBC-SD increased significantly (P<0.05), while after knocking down MUC16, the cell viability and migration ability of GBC-SD decreased significantly (P<0.05). In addition, after knocking down MMP-9, the cell viability and migration ability of GBC-SD also decreased significantly (P<0.05). However, when MUC16 was overexpressed and MMP-9 was knocked down, there was no significant difference in cell viability and migration ability between GBC-SD and the control group (P>0.05). Conclusion MUC16 activates PI3K/Akt pathway to promote the expression of MMP-9 protein, thereby enhancing the cell viability and migration of GBC-SD.

Key words: Mucin 16, Phoshoinositide 3-kinase/protein kinase B pathway, Matrix metalloproteinase-9, Gallbladder cancer cell, Western blotting, Real-time PCR, Human

LI Xin TIAN Mi PENG Bing HE Li-li. Mucin 16 regulating activity and migration of gallbladder cancer cells through phosphoinositide 3-kinase/protein kinase B pathway[J]. Acta Anatomica Sinica, 2019, 50(5): 613-619.

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