Effects of Klotho on the proliferation and differentiation of rat bone marrow mesenchymal stem cells

doi:10.16098/j.issn.0529-1356.2019.06.005

Abstract

Abstract:

Objective To investigate the effect of antiaging gene Klotho on the proliferation and differentiation of rat bone marrow mesenchymal stem cells(BMSCs). Methods Rat BMSCs overexpressing secreted Klotho(sKL) were cultured and constructed in vitro. MTT assay was used to detect the cell proliferation. Western blotting was used to measure the expression of sKL in the cells and medium of three groups. As markers of aging, P53 and P21 in three cells were also detected. BMSCs were induced to osteogenic differentiation by osteogenic induction medium, the result of differentiation was observed by alkaline phosphatase(ALP) staining; BMSCs were induced to adipogenic differentiation by adipogenic induction medium, the result of differentiation was identified by oil red O staining. Results Western blotting showed that the expression of sKL increased significantly in the sKL group (P<0.05), in addition, the expression of P53 and P21 decreased significantly in the sKL group(P<0.05). MTT assay indicated that there was no significant differences about absorbance value between sKL group and control group(P>0.05). The ability of osteogenic differentiation and adipogenic differentiation was significantly stronger than that of the control group(P<0.05). Conclusion sKL effectively enhances the anti-aging ability of BMSCs, meanwhile it also promotes osteogenic and adipogenic differentiation of BMSCs. Besides, this study also suggests that Klotho may have no effect on the proliferation of BMSCs.

Key words: Bone marrow mesenchymal stem cell, Secreted Klotho, Proliferation, Differentiation, Western blotting

LI Xiu-quan BAI Shu-ling AO Qiang WANG Xiao-hong CHANG Shi-jie TIAN Xiao-hong TONG Hao FAN Jun. Effects of Klotho on the proliferation and differentiation of rat bone marrow mesenchymal stem cells[J]. Acta Anatomica Sinica, 2019, 50(6): 729-734.

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