Effect of porcine kidney epithelial cells type Ⅰ interferon receptor 1 knockdown on pseudorabies virus replication

doi:10.16098/j.issn.0529-1356.2019.06.007

Abstract

Abstract:

Objective To investigate the effect of type Ⅰ interferon receptor 1 (IFNAR1) on pseudorabies virus (PRV) replication. Methods A stable cell line in which porcine kidney epithelial cells (PK15) knocked out the IFNAR1 gene was constructed using a lentiviralmediated CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats, CRISPR) gene editing technique. The function of IFNAR1 was verified by cell viability assay, fluorescence observation, flow cytometry detection, titer determination, and Real-time PCR. Results With the prolongation of PRV infection, knocking out the IFNAR1 gene can significantly promote transcription of PRV-TK mRNA, translation of PRV-gE protein, and virulence of progeny virus. Conclusion IFNAR1 plays an important role in inhibiting the proliferation of PRV.

Key words: Porcine renal epithelial cell, Type Ⅰ interferon receptor 1, Pseudorabies virus, Gene editing, Western blotting, Real-time PCR, Porcine

SHAO Ke-yu ZHANG Shuang DUAN Li-fang MA Ying-xian GUO Yu-kun LI Jia-jia LIU Xiao-he DU Yong-kun CHU Bei-bei. Effect of porcine kidney epithelial cells type Ⅰ interferon receptor 1 knockdown on pseudorabies virus replication[J]. Acta Anatomica Sinica, 2019, 50(6): 741-746.

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