Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland

doi:10.16098/j.issn.0529-1356.2020.02.002

Abstract

Abstract:

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat adrenal gland and to detect the effect of cyclic intermittent hypoxia (CIH) on the expression of BACE1. Methods The expression and localization of BACE1 in rat adrenal gland were detected by Western blotting and immunohistochemistry. Sixteen male Sprague-Dawley (SD) rats were randomly divided into two groups: control group and CIH group, 8 rats in each group. The protein levels of BACE1 and tyrosine hydroxylase (TH) in rat adrenal medulla were detected by Western blotting after CIH 2 weeks treatment. Results BACE1 was mainly localized in rat adrenal medullary nerve fibers. Compared with the control group, BACE1 protein level decreased and TH protein level increased in the adrenal medulla in the CIH group.

Conclusion BACE1 is located in rat adrenal medullary nerve fibers. The decreased level of BACE1 may participate in slowing down the excessive enhancement of sympathetic activity induced by CIH.

Key words: β-site amyloid precursor protein cleaving enzyme 1, Adrenal gland, Tyrosine hydroxylase, Cyclic intermittent hypoxia, Western blotting, Rat

CLC Number:

FAN Ya-nan LI Chao-hong JIA Xiang-lei LIU Yu-zhen. Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland[J]. Acta Anatomica Sinica, 2020, 51(2): 162-166.

References

［1］ Munro KM, Nash A, Pigoni M, et al. Functions of the Alzheimer’s disease protease BACE1 at the synapse in the central nervous system［J］. Mol Neurosci, 2016, 60(3):305-315.

［2］ Sadleir KR, Kandalepas PC, Buggia-Prévot V, et al. Presynaptic dystrophic neuritis surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer’s disease［J］. Acta Neuropathol, 2016, 132(2):235-256.

［3］ Xiangyou Hu, Caitlin W Hicks, Wanxia He, et al. BACE1 modulates myelination in the central and peripheral nervous system［J］. Nat Neurosci, 2006, 9(12):1520-1525.

［4］ Tallon C, Farah MH. Beta secretase activity in peripheral nerve regeneration［J］. Neural Regen Res, 2017, 12(10): 1565-1574.

［5］ García-Río F, Racionero MA, Pino JM, et al. Sleep apnea and hypertension［J］. Chest, 2000, 117(5):1417-1425.

［6］ Peng YJ, Yuan G, Khan S, et al. Regulation of hypoxia-inducible factor-α isoforms and redox state by carotid body neural activity in rats［J］. J Physiol, 2014, 592(17):3841-3858.

［7］ Lin Gao, Patricia Ortega-Sáenz, José López-Barneo. Acute oxygen sensing-role of metabolic specifications in peripheral chemoreceptor cells［J］. Respir Physiol Neurobiol, 2019, 265:100-111.

［8］ Heindl M, Tuennemann J, Sommerer Ⅰ, et al. Loss of BACE1 in mice does not alter the severity of caerulein induced pancreatitis［J］. PLoS One, 2015, 10(5): e0125556.

［9］ Zhu K, Xiang X, Filser S, et al. Beta-site amyloid precursor protein cleaving enzyme 1 inhibition impairs synaptic plasticity via seizure protein 6［J］. Biol Psychiatry, 2018, 83(5):428-437.

［10］ Lumb R, Tata M, Xu X, et al. Neuropilins guide preganglionic sympathetic axons and chromaffin cell precursors to establish the adrenal medulla［J］. Development, 2018, 145(21):dev162552.

［11］ Fletcher EC. Physiological consequences of intermittent hypoxia: systemic blood pressure［J］. Appl Physiol, 2001, 90(4):1600-1605.

［12］ Nanduri J, Peng YJ, Yuan G, et al. Hypoxia-inducible factors and hypertension: lessons from sleep apnea syndrome［J］. Mol Med (Berl), 2015, 93(5):473-480.

［13］ Gao L, Bonilla-Henao V, García-Flores P, et al. Gene expression analyses reveal metabolic specifications in acute O2-sensing chemoreceptor cells［J］. J Physiol, 2017, 595(18):6091-6120.

［14］ Liu JP, Long GK. Histological study on the nerve supply of the human adrenal gland［J］. Acta Anatomica Sinica, 1964, 7(3):344-351.(in Chinese)

刘经平, 龙桂开. 关于人肾上腺的神经供给的组织学研究［J］. 解剖学报, 1964, 7(3):344-351.

［15］ Yokotani K, Okada S, Nakamura K, et al. Characterization of functional nicotinic acetylcholine receptors involved in catecholamine release from the isolated rat adrenal gland［J］. Eur J Pharmacol, 2002, 446(1-3):83-87.

［16］ Lim J, Kim HI, Bang Y, et al. Hypoxia-inducible factor-1α upregulates tyrosine hydroxylase and dopamine transporter by nuclear receptor ERRγ in SH-SY5Y cells［J］. Neuroreport, 2015, 26(6):380-386.

［17］ Kumar GK, Nanduri J, Peng YJ, et al. Neuromolecular mechanisms mediating the effects of chronic intermittent hypoxia on adrenal medulla［J］. Respir Physiol Neurobiol, 2015, 209:115-119.

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