Objective  To investigate the effect of alkaline phosphatase liver/bone/kidney (ALPL) overexpression on ventricular remodeling after acute myocardial infarction (MI).   Methods  Thirty six 8-week-old male C57BL/6 J mice were randomly divided into sham group（sham）, MI group (MI+Adv-EGFP) and ALPL overexpression group (MI+Adv-ALPL) with 12 mice in each group. Two weeks after MI，cardiac function of mice was detected by echocardiography, pathological changes was detected by HE staining. ALPL mRNA expression in mouse heart was detected by Realtime PCR. The levels of ALPL, α-smooth muscle actin (α-SMA)，collagen Ⅰ (ColⅠ) and collagen Ⅲ (ColⅢ) protein were detected by Western blotting. The collagen volume fraction (CVF) and the ratio of ColⅠ/ColⅢ were measured by polarizing method  with Sirius red staining.  Results  Compared with MI+Adv-EGFP group, the left ventricular ejection fraction (LVEF) (%) and left ventricular fractional shortening (LVFS) (%) of the heart in MI+Adv-ALPL group were reduced significantly (P<0.05). Hearts in the ALPL overexpression group showed myocardial fiber broken, and disordered and fibrous scars were obvious in myocardial infarction area. The expression of ALPL and the expression of α-SMA, ColⅠ and ColⅢ protein in MI+Adv-ALPL group increased significantly (P<0.05), and the proportion of ColⅠ/ColⅢ in MI+Adv-ALPL group increased significantly (P<0.05).Compared with MI+Adv-EGFP group, the CVF and the proportion of the ratio of ColⅠ/ColⅢ in the MI+Adv-ALPL group increased significantly (P<0.05).   Conclusion  ALPL overexpression can promote the ventricular pathological remodeling after acute myocardial infarction in mice.