Objective  To investigate the effects of microRNA (miR)-27a on proliferation, apoptosis and invasion of cervical cancer cells by targeting F-box and WD repeat domain containing protein 7( FBXW7) expression.   Methods  Thirty cases of cervical cancer and paracancerous tissues and 30 cases of normal tissues were used in the experiment. The expression of miR-27a in cervical cancer, paracancerous tissue, normal cervical tissue, cervical cancer cells (SiHa, Caski, HeLa, HCC94) and cervical squamous epithelial immortalized cell H8 were detected by Real-time PCR. MiR-27a inhibitor and its negative control were transfected into SiHa cells by liposome transfection. CCK-8 assay, flow cytometry and Transwell assay were used to detect the effects of miR-27a on the proliferation, cell cycle, apoptotic rate and invasive ability of SiHa cells.Bioinformatics was used to predict the targeting gene of miR-27a. Double luciferase reporter gene assay combined with Western blotting was used to verify the targeting regulation of miR-27a on FBXW7.   Results  Compared with the normal cervical tissues and the adjacent tissues, the expression of miR-27a was higher in cervical cancer tissues (P<0.05); Compared with the cervical squamous epithelial immortalized cells H8, the expression of miR-27a in cervical cancer cells SiHa, Caski, HeLa and HCC94 was higher (P<0.05). Inhibiting the expression of miR-27a in SiHa cells could significantly reduce the proliferation activity of cells (P<0.05), increase the proportion of G0/G1 cells (P<0.05), decrease the proportion of G2/M cells (P<0.05), increase the apoptosis rate (P<0.05), and inhibit the invasive ability of cells (P<0.05). Bioinformatics predicted that FBXW7 might be a target regulatory gene of miR-27a. Double luciferase reporter gene assay showed that miR-27a could specifically bind to the 3’UTR region of FBXW7 (P<0.05), and negatively regulate the expression of FBXW7 protein (P<0.05).   Conclusion  MiR-27a plays an oncogene role in the occurrence and development of cervical cancer. Inhibiting the expression of miR-27a can significantly inhibit the malignant biological behavior of cervical cancer cells, and its mechanism may be related to targeted regulation of FBXW7 expression.