Effects of acetyl-CoA carboxylase 1 on proliferation, migration and invasion of glioma cell line U87

doi:10.16098/j.issn.0529-1356.2022.03.007

Abstract

Abstract:

Objective To explore the effect of acetyl-CoA carboxylase 1(ACC1) on cell proliferation, migration and invasion of human glioma cell line U87. Methods Western blotting was performed to examine endogenous ACC1 expression in human glioma cell lines U87, U251 and U373. ACC1 overexpression plasmid and the plasmid vector were transiently transfected into U87 cells. The level of ACC1 in control and ACC1 overexpression cells was examined by Western blotting. The effect of ACC1 on U87 cells migration and invasion was detected by Transwell assay. The effect of ACC1 on U87 cells scratch healing ability was detected by scratch test. The effect of ACC1 on U87 cells proliferation was investigated by MTT assay. Western blotting was conducted to detect the level changes of proteins. Results Among three human glioma cell lines U87, U251 and U373, endogenous ACC1 level in U87 cells was lower than that in other two cell lines. ACC1 overexpression inhibited U87 cell proliferation, as well as cell migration, invasion and scratch healing ability (P<0.05). Vimentin, fibronectin, urokinase type plasminogen activator (uPA), Bcl-2, cyclin B, cyclin D and p-STAT3 were down-regulated (P<0.05), P21 was up-regulated (P<0.05) after ACC1 overexpression. Conclusion These results suggest that ACC1 suppresses the proliferation, migration and invasion of human glioma cells, probably by inhibiting STAT3 activity.

Key words: Acetyl-CoA carboxylase 1, U87 cell, Proliferation, Migration, Invasion, Western blotting, Human

CLC Number:

R739.41

WANG Yang ZHAO Bao-sheng LI Chao-hong LIU Yu-zhen. Effects of acetyl-CoA carboxylase 1 on proliferation, migration and invasion of glioma cell line U87[J]. Acta Anatomica Sinica, 2022, 53(3): 317-322.

References

［1］Perry A, Wesseling P. Histologic classification of gliomas［J］. Handb Clin Neurol, 2016, 134: 71-95.

［2］Louis DN, Perry A, Reifenberger G, et al. The 2016 World Health Organization Classification of Tumors of the Central Nervous System: a summary［J］. Acta Neuropathol, 2016, 131(6): 803-820.

［3］Roumeliotis TI, Williams SP, Gonalves E, et al. Genomic determinants of protein abundance variation in colorectal cancer cells［J］. Cell Rep, 2017, 20(9): 2201-2214.

［4］Mounier C, Bouraoui L, Rassart E. Lipogenesis in cancer progression (review)［J］. Int J Oncol, 2014, 45(2): 485-492.

［5］Wang C, Rajput S, Watabe K, et al. Acetyl-CoA carboxylase-a as a novel target for cancer therapy［J］. Front Biosci (Schol Ed), 2010, 2: 515-526.

［6］Yahagi N, Shimano H, Hasegawa K, et al. Co-ordinate activation of lipogenic enzymes in hepatocellular carcinoma［J］. Eur J Cancer, 2005, 41(9): 1316-1322.

［7］Rios Garcia M, Steinbauer B, Srivastava K, et al. Acetyl-CoA carboxylase 1-dependent protein acetylation controls breast cancer metastasis and recurrence［J］. Cell Metab, 2017, 26(6): 842-855.

［8］Chandrashekar DS, Bashel B, Balasubramanya SAH, et al. UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses［J］. Neoplasia, 2017, 19(8): 649-658.

［9］Li G, Zhang Z, Wang R, et al. Suppression of STIM1 inhibits human glioblastoma cell proliferation and induces G0/G1 phase arrest［J］. J Exp Clin Cancer Res, 2013, 32(1): 20.

［10］Yu XH, Ren XH, Liang XH, et al. Roles of fatty acid metabolism in tumourigenesis: Beyond providing nutrition (review) ［J］. Mol Med Rep, 2018, 18(6): 5307-5316.

［11］Ye B, Yin L, Wang Q, et al. ACC1 is overexpressed in liver cancers and contributes to the proliferation of human hepatoma Hep G2 cells and the rat liver cell line BRL 3A［J］. Mol Med Rep, 2019, 19(5): 3431-3440.

［12］Li EQ, Zhao W, Zhang C, et al. Synthesis and anti-cancer activity of ND-646 and its derivatives as acetyl-CoA carboxylase 1 inhibitors［J］. Eur J Pharm Sci, 2019, 137: 105010.

［13］Jones JE, Esler WP, Patel R, et al. Inhibition of acetyl-coA carboxylase 1 (ACC1) and 2 (ACC2) reduces proliferation and De novo lipogenesis of EGFRvⅢ human glioblastoma cells［J］. PLoS One, 2017, 12(1): e0169566.

［14］Wellen KE, Thompson CB. A two-way street: reciprocal regulation of metabolism and signaling［J］. Nat Rev Mol Cell Biol, 2012, 13(4): 270-276.

［15］Kuchta K, Towpik J, Biernacka A, et al. Predicting proteome dynamics using gene expression data［J］. Sci Rep, 2018, 8(1): 13866.

［16］Costanzo M, Kuzmin E, Leeuwen JV, et al. Global genetic networks and the genotype-to-phenotype relationship［J］. Cell, 2019, 177(1): 85-100.

［17］Galdieri L, Vancura A. Acetyl-CoA carboxylase regulates global histone acetylation［J］. J Biol Chem, 2012, 287(28): 23865-23876.

［18］Chow JD, Lawrence RT, Healy ME, et al. Genetic inhibition of hepatic acetyl-CoA carboxylase activity increases liver fat and alters global protein acetylation［J］. Mol Metab, 2014, 3(4): 419-431.

［19］Ouédraogo ZG, Biau J, Kemeny JL, et al. Role of STAT3 in genesis and progression of human malignant gliomas［J］. Mol Neurobiol, 2017, 54(8): 5780-5797.

［20］Rébé C, Ghiringhelli F. STAT3, a master regulator of anti-tumor immune response［J］. Cancers (Basel), 2019, 11(9): 1280.

［21］Wang J, Yang Y, Yang N, et al. Expression of phosphorylated-signal transduction and activator of transcription 3 (Y705) in adenomyosis and their clinical significances［J］. Acta Anatomica Sinica, 2016, 47(2):261-267. (in Chinese)

王静，杨阳，杨宁，等. 磷酸化信号转导和转录激活因子3(Y705)在子宫腺肌症中的表达及其临床意义［J］. 解剖学报，2016，47(2): 261-267.

［22］Piperi C, Papavassiliou KA, Papavassiliou AG. Pivotal role of STAT3 in shaping glioblastoma immune microenvironment［J］. Cells, 2019, 8(11): 1398.

［23］Avalle L, Camporeale A, Camperi A, et al. STAT3 in cancer: a double edged sword［J］. Cytokine, 2017, 98: 42-50.

［24］Sestito R, Madonna S, Scarponi C, et al. STAT3-dependent effects of IL-22 in human keratinocytes are counterregulated by sirtuin 1 through a direct inhibition of STAT3 acetylation［J］. FASEB J, 2011, 25(3): 916-927.

［25］Lv D, Li Y, Zhang W, et al. TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma［J］. Nat Commun, 2017, 8(1): 1454.

[1]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[2]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[3]	LIU Xiao-li XU Lin WU Ai-xue WEN Cai-yun LI Ru-hua WU An-ting CHEN Dai-qian CHEN Cheng-chun. Analysis of cerebral gray matter structure in multiple sclerosis and neuromyelitis optica [J]. Acta Anatomica Sinica, 2024, 55(1): 17-24.
[4]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[5]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[6]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[7]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[8]	BAI He TENG Ye FENG Lei MENG Hai-wei1 TANG Yu-chun LIU Shu-wei. 3D hippocampal segmentation based on spatial and frequency domain features adaptive fusion and inter-class boundary region enhancement [J]. Acta Anatomica Sinica, 2024, 55(1): 73-81.
[9]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[10]	YIN Shi-qin YANG Si-yi WANG Rui-han YOU Gui-xuan YANG Ying-qiu ZHANG Lei. Distal tibiofibular syndesmosis fibular notch typing and its clinical significance based on CT [J]. Acta Anatomica Sinica, 2024, 55(1): 82-87.
[11]	YANG Yang SHI Jun LI Kun MA Yuan ZHANG Shao-jie HOU Er-fei WANG Chao-qun CHEN Jie WANG Xing LI Zhi-jun. Finite element analysis of cervical intervertebral discs after removing different ranges of uncinate processes [J]. Acta Anatomica Sinica, 2024, 55(1): 88-97.
[12]	SUN Lei WANG Xing-yu XIE Shui-hua. Risk analysis of re-fracture after percutaneous kyphoplasty in elderly patients with osteoporotic thoracolumbar compression fractures and construction of acolumnar graph prediction model#br# [J]. Acta Anatomica Sinica, 2024, 55(1): 98-104.
[13]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[14]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[15]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.