Protective effects of ethyl acetate extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons induced by lipopolysaccharides

doi:10.3969/j.issn.0529-1356.2013.05.007

Abstract

Abstract:

Objective To investigate the protective effects of different doses of Ethyl Acetate (EA) Extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons of the PD mouse which was induced by lipopolysaccharides (LPS). Methods Adult male Kunming(KM) mice were used in the present study, LPS was injected into the substantia nigra compacta(SNc) to duplicate mouse model of PD. A tatal of 30 KM mice were randomly divided into five groups: the sham group, the LPS control group, low dose experimental group(LPS+100mg/kg), medial dose experimental group (LPS+200mg/kg) and high dose experimental group(LPS+400mg/kg), six mice per group. The immunofluorescence methods were used to label the dopaminergic neurons and astrocytes in SN. The dopaminergic positive neurons were counted and analyzed with statistics. Results The dopaminergic neuron numbers in LPS control group were markedly decreased in comparison with sham group (P<0.05), and the dopaminergic neurons appeared ovale, and the neurite was shorter than the sham group. Astrocytes displayed the activated state with larger bodies, thickened and shortened enation in the SN of LPS control group compared with the sham group. The dopaminergic neurons were increased significantly in the low and median dose groups compared with LPS control group(P<0.05), the density of astrocytes was lower. There was no significant difference of dopaminergic neurons between high dose group and LPS control group(P>0.05). Conclusion The low and median groups may protect the degeneration of dopaminergic neurons in SNc by depressing the activity of astrocytes in SNc.

Key words: Parkinson’s disease, Lipopolysaccharides, Dopaminergic neuron, Ethyl acetate extract fromChroogomphus rutilus\ Stereotaxic injection, Mice

HU Yu CHEN Xiao WANG Li-an GUAN Zhen-long WANG Yan-qin*. Protective effects of ethyl acetate extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons induced by lipopolysaccharides[J]. AAS, 2013, 44(5 ): 621-625.

References

［1］ Dai YCh, Zhou LW, Yang ZhL, et al.A revised checklist of ediblefungi in China［J］.Mycosystema, 2010;29(1): 1-21.（in Chinese）
戴玉成，周丽伟，杨祝良，等. 中国食用菌名录［J］. 菌物学报，2010，29(1)：1-21. 
［2］ Ge J. The research of the pine mushroom’s effect on cancer in Japan［J］.Edible Fungi of China , 1996;15(5): 38.（in Chinese）
戈杰.日本研究松蘑治癌［J］.中国食用菌，1996;15(5): 38. 
［3］ Zhuang QZh, Pine mushroom-fight against electromagnetic radiation and make women’s face more pretty［J］.The Science of Diet, 2007;(4): 24.（in Chinese）
庄乾竹.松蘑—对抗电磁辐射靓化女人容颜［J］. 饮食科学，2007;(4): 24. 
［4］Sun YX, Li X, Yang JF, et al. Water-soluble polysaccharide from the fruiting bodies of Chroogomphus rutilus (Schaeff.: Fr.) O.K. Miller: isolation, structural features and its scavenging effect on hydroxyl radical［J］.Carbohydr Polym,2010;80(3): 720-724.
［5］Weber CA, Ernst ME, Antioxidants, supplements, and Parkinson’s disease［J］. Ann Pharmacother , 2006;40(5): 935-938.
［6］ Yang HH, Xu PY, Liu ZhL, et al. Ganoderma lucidum polysaccharides protects rat primary dopaminergic neurons induced by lipopolysaccharides［J］. Journal of Sun Yat-sen University (Medical Sciences), 2006,27(3): 241-245.（in Chinese）
杨海华，徐评议，刘焯霖，等.灵孢多糖对脂多糖诱导的原代多巴胺能神经元变性的保护作用［J］.中山大学学报，2006，27(3): 241-245. 
［7］ Zhang XQ，Sun H，Wang LA，et al. Protective effects of crudepolysaccharides from Chroogomphus rutilus on dopaminergic neurons impaired by MPTP in mice［J］. Mycosystema,2011,30(1):77-84.（in Chinese）
张雪倩，孙红，王立安，等.色钉菇粗多糖对小鼠DA能神经元 MPTP 损伤的保护作用［J］.菌物学报，2011;30(1)：77-84. 
［8］Hirsch EC, Vyas S, Hunot S. Neuroinflammation in Parkinson’s disease［J］. Parkinsonism Relat Disord, 2012,18 (Suppl) 1:S210-212.
［9］Dutta G, Zhang P, Liu B. The lipopolysaccharide Parkinson's disease animal model: mechanistic studies and drug discovery［J］. Fundam Clin Pharmacol, 2008, 22(5):453-464.
［10］Li J. The relationship of astrocytes activation stimulated by LPS and degeneration of dopaminergic neurons in substantia nigra in rats ［D］. Suzhou Univesity,2006:1-37.（in Chinese）
李洁.脂多糖诱导的大鼠星型胶质细胞激活与黑质多巴胺能神经元损伤之间的关系［D］. 苏州大学,2006:1-37. 
［11］Rappold PM，Tieu K. Astrocytes and therapeutics for Parkinson’s disease［J］. Neurotherapeutics, 2010，7(4)：413-423.

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[10]	ZHANG Lin-bing HAN Yan-yan QIU Shi XU Shan ZUO Ji LIU Wen. Expression and alteration induced by MPTP / MPP⁺of mitochondrial transcription termination factors family [J]. Acta Anatomica Sinica, 2017, 48(1): 19-24.
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[12]	WANG Xiao-xiao FU Wen-yu* ZHUANG Wen-xin LI Feng-jie WANG Qian LIU Jin-cheng. Lentiviral vector mediated Nurrl gene-modified mesenchymal stem cells for the treatment of Parkinson’s disease rat model [J]. AAS, 2015, 46(6): 742-749.
[13]	WANG Qiong-sa, QI Shuang-shuang, ZHOU Peng, ZHANG Chi-hao, CUI Huai-rui, CHEN Shi-xin, SUN Chen-you* . Effect of allopregnanolone on the dopaminergic neurons in the substantia nigra of Alzheimer’s disease mice  [J]. AAS, 2013, 44(2 ): 176-181.
[14]	. Expression change of the secreted frizzled-related protein 4 in spinal cord of amyotrophic lateral sclerosis transgenic mice [J]. , 2012, 43(4): 439-444.
[15]	. Morphological changes of the nigrostriatal pathway in a mouse model of Parkinson’s disease [J]. , 2012, 43(3): 293-298.