Protective effect of flavonoids of rosa roxburghii tratt on myocardial cytotoxicity induced by doxorubicin

doi:10.16098/j.issn.0529-1356.2019.01.009

Abstract

Abstract:

Objective To investigate the effect and mechanism of flavonoids of rosa roxburghii tratt(FRRT) on apoptosis in doxorubicin(DOX)-induced cardiotoxicity. Methods Myocardial cells were isolated and cultured from 26 newborn rats of 1-3 days or H9C2 myocardial cell line. The myocardial cytotoxicity model was established by incubating myocardial cells with a concentration of 5 μmol/L DOX, and FRRT group were intervened with FRRT incubation in advance. Ultrastructure of H9C2 myocardial cells were observed with transmission electron microscope. The expressions of Bax, P53 and Bcl-2 in myocardial cells were detected with Western blotting and immunofluorescence, and the results of Western blotting were analyzed with semi-quantitatively. Results Transmission electron microscopy showed that myocardial cells had a large number of autophagic vacuoles and lipofuscin, and cells were in poor condition in DOX group, the myocardial cells of the FRRT+DOX group had no structure of lipofuscin, and only a small amount of autophagic vacuoles. The results of immunofluorescence showed that the expressions of Bax and P53 were positive, and Bcl-2 was weakly positive in DOX group. The expression of Bax and P53 were not obvious, and Bcl-2 was strongly positive in FRRT+DOX group. The results of Western blotting showed that the myocardial cells were incubated with DOX for 18hours, the expression level of Bax was the highest, and the difference had statistical significance between groups (P<0.01).The expressions of Bax were increased in DOX group,and reduced in FRRT+DOX group, and the difference had statistical significance between groups (P<0.05). The expressions of P53 were increased in DOX group,and reduced in FRRT group, and the difference without statistial significance between groups(P>0.05). The expressions of Bcl-2 were reduced in DOX group, and increased in FRRT+DOX group, the difference had statistical significance between groups(P<0.05, P<0.01). Conclusion FRRT protects cardiomyocytes by down-regulating DOX-induced cardiomyocyte apoptosis.

Key words: Myocardial cells, Flavonoids of rosa roxburghii tratt, Doxorubicin, Immunofluorescence, Western blotting, Rat

YUAN Hui-fang ZHANG Yong-chun CAI Xin-hua XU Ping CHEN Hui. Protective effect of flavonoids of rosa roxburghii tratt on myocardial cytotoxicity induced by doxorubicin[J]. Acta Anatomica Sinica, 2019, 50(1): 49-55.

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