Abstract:

Objective To investigate the effect of carmofur on the proliferation and invasion of cervical cancer cell line HeLa and to study its mechanism. Methods Carmofur was prepared into different concentrations (0.4 mg/L, 0.8 mg/L, and 1.2 mg/L) and the control group was set. Cell counting kit-8(CCK-8) method was used to detect the viability of the cells. Transwell technique was used to detect cell invasion. The expression levels of matrix metalloproteinase(MMP)-7, β-catenin and P120 catenin(P120 ctn) and mRNA were detected by Western blotting and Real-time PCR. Results Compared with the control group, the cell proliferation ability decreased with the increase of the concentration of carmofur in the drug treatment group, and the cell proliferation was inhibited after 24 hours of carmofur exposure, and the cell proliferation rate decreased significantly with the prolongation of the action time (all P<0.05). Compared with the control group,carmofur group inhibited the invasion of HeLa cells (P<0.05). Compared with the control group, the carmofur group decreased MMP-7, β-catenin and P120 catenin levels and mRNA levels (P<0.05). Conclusion HCFU can inhibit the proliferation of HeLa cells, and its mechanism might be related to the downregulation of MMP-7, β-catenin and P120 catenin expression levels.

Key words: Carmofur, HeLa cell, Proliferation, Invasion, Western blotting, Human