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    Review
    Structure of calcium/calmodulin-dependent protein kinase type Ⅱ and its role in nervous system
    WANG Tong-tong CHEN Zhi-chi YE Xin FU Wei-da CHEN Meng-jiao LI Jun-nan SUN Chen-you
    2019, 50 (3):  395-399.  doi: 10.16098/j.issn.0529-1356.2019.03.022
    Abstract ( )   PDF (863KB) ( )  

    Calcium (Ca2+ ) has long been recognized as a crucial intracellular messenger attaining stimuli specific cellular outcomes via localized signaling. Ca2+ binding proteins, such as calmodulin (CaM) and its target proteins are key to Ca2+ -dependent signaling events. Calcium/calmodulin-dependent protein kinase type Ⅱ (CaMKⅡ) is a highly abundant polymer enzyme comprising a significant fraction of total protein in mammalian forebrain and forming a major component of the postsynaptic density. In recent years, studies have shown that CaMKⅡ contains four subtypes of α, β, γ and δ, in which CaMKⅡ α and β are mainly expressed in nerve tissues and γ and δ are expressed in the whole body. They are essential for certain synaptic plasticity and memory consolidation processes, both in the central nervous system and in the excitability of the nervous system and in some neurological diseases. CaMKⅡ may play an important role in the pathogenesis of some nervous system diseases. Previous studies have also shown tha CaMKⅡδ plays an important role in promoting neuronal survival. The structure of CaMKⅡ and its role in nervous system and its relationship with related nervous system diseases will be reviewed.

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    Research progress on the balance regulation of osteogenic differentiation and adipogenic differentiation in bone marrow mesenchymal stem cell
    ZHAN Xiu-wen YANG Lei ZHENG Mei-rong WANG Xin-ping XU Xiao-yuan
    2019, 50 (3):  400-404.  doi: 10.16098/j.issn.0529-1356.2019.03.023
    Abstract ( )   PDF (997KB) ( )  

    Osteoporosis (OP), a systemic bone metabolism disease, mainly manifested in the decrease of bone mass, the increase of bone fragility and the microstructure degeneration of the bone. Along with the in-depth research of the pathogenesis of osteoporosis, the imbalance differentiation of bone marrow mesenchymal stem cell (BMSCs) (Osteogenic differentiation decrease and adipogenic differentiation increase) is the main reason that causes osteoporosis. In this paper, we summarize the signal pathways of osteogenic differentiation and adipogenic differentiation of BMSCs. Better understand these signal pathways is conducive to elucidate and treat osteoporosis.

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    Technology and Methodology
    Effects of different fixing agents on tumor cell morphology and membrane permeability
    WANG Xiao-jie ZHANG Yu-xiang
    2019, 50 (3):  392-394.  doi: 10.16098/j.issn.0529-1356.2019.03.021
    Abstract ( )   PDF (5349KB) ( )  

    Objective To observe the effect of different fixative solutions on cancer cell morphology and membrane permeability. Methods Human pancreatic acinar epithelial carcinona(HPAC) cells of human pancreatic cancer and HeLa cells of human cervical cancer were fixed with 4 fixation solutions: freshly prepared 0.25% paraformaldehyde solution; Freshly prepared 4% paraformaldehyde solution; 75% ethanol solution; 90% ethanol solution. The fixation time is 30 minutes. PBS solution and complete medium were used as the controls. Cell morphology of each group was observed under optical microscope. Changes in cell membrane permeability were observed by fluorescence staining with 7-aminoactinomycin (7-AAD), which is not cell membrane permeable in intact cells but permeable in damaged cells. Hoechst33342 was used for staining both intact and damaged cells. Results The cells in the complete medium group were similar to unfixed cells in morphology, and the fluorescence staining of 7-AAD was the weakest. The cells in the complete medium group have typical cell morphology and low 7-AAD permeability. The 0.25% paraformaldehyde solution group had similar cell morphology to the complete medium group, and the 7-AAD fluorescence staining was weak. The morphology of cells in the 4% paraformaldehyde solution group was typical, but the fluorescence staining of 7-AAD was strong. The cells in the 90% ethanol solution group showed swelling, with a larger volume than the unfixed cells and a stronger fluorescence staining of 7-AAD. The cell swelling in 75% ethanol solution group was not as obvious as that in 90% ethanol solution group, and the fluorescence staining of 7-AAD was strong. The cells in PBS group were round, and the fluorescence staining of 7-AAD was strong. Conclusion 0.25% paraformaldehyde solution can not only fix tumor cells, but also maintain the integrity of cell membrane.

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    Anthropology
    Evolutionary trends of stature in Pleistocene China
    DU Bao-pu ZHANG Li-zhao ZHAO Ling-xia
    2019, 50 (3):  351-357.  doi: 10.16098/j.issn.0529-1356.2019.03.015
    Abstract ( )   PDF (1032KB) ( )  

    Objective To study the evolutionary trends in the stature of Pleistocene hominins from China. Methods We took the indirect method of stature estimation from fragments of long bones: maximum length of the long bones was directly estimated from measurements of its fragments, and thereafter the stature was reconstructed from the estimated maximum length. Results The result showed that the stature variation was 155.4-169.2 cm for H. erectus (n=4) and 155.2-171.7 cm for early modern humans (n=12), and only 166.1 cm for one female in archaic Homo sapiens. The stature of Liujiang and Lijiang hominins located in the southeast was lower than other early modern humans from North China. Conclusion The stature variation is similarly from H. erectus to early modern humans, while the average stature seems to have increased continuously. We find that the geographical variation observed in modern humans has appeared in early modern humans. In addition, there is no significant difference between early modern humans and Neolithic populations.

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    Dermatoglyphics of Han people living in Huaibei plains on Anhui
    LI Jin LIU Zai-qun HUA Zhao-he ZHANG Shou-fang LU Yi-lin
    2019, 50 (3):  358-362.  doi: 10.16098/j.issn.0529-1356.2019.03.016
    Abstract ( )   PDF (862KB) ( )  

    Objective To report hand dermatoglyphics parameters of Han individuals living in Huaibei plains of Anhui. Methods Fingerprints and palmar prints were taken with informed consent, collected sample by ninhydringourmet powder method, appraised and counted with stereomicroscope. Total samples were 1590 Han,including 796 males and 794 females. Results The result were obtained from finger pattern types, and order of decreasing, combination, fingerprint index, TFRC, a-bRC, a-bRD, atd angle, tPD, true patterns of palm and flexion creases etc model sample. Conclusion Its dermatoglyphic database will be useful for future studies in anthropology, genetics and medicine.

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    Mandibular torus variation of Chinese ancient population
    LI Hai-jun LIU Lin-ru XU Jin-ru DAI Cheng-ping
    2019, 50 (3):  363-368.  doi: 10.16098/j.issn.0529-1356.2019.03.017
    Abstract ( )   PDF (856KB) ( )  

    Objective To present the performance of mandibular torus of Chinese in the Holocene,to analyze the age variation and the intercrowd variation of the mandibular torus and to explore related reasons. Methods Image comparison and combination description of the mandibular torus with seeds of the specified size, such as peanuts, soybeans, pumpkin seeds, etc,and compare them in different eras,genders,and locations. Results The result showed that the male’s mandibular torus was distinctively stronger than that of the female. It was larger in the Neolithic Age than in the Bronze and Iron Age and the size decreased further as human beings moved into the modern era. In comparison of mandibular torus in different locations, Jiangjialiang was the largest, Xinjiang was the second, and Tuchengzi and Lamadong were the smallest. Conclusion During the Holocene period, the degree of bulging of the mandibular torus is reduced from time to time, and the difference in the performance of the mandibular torus may be closely related to the difference in mastication pressure caused by food differences.

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    Relationship between body iron and elevated blood glucose in Mosuo people
    SU Ming-jie LI Hui
    2019, 50 (3):  369-373.  doi: 10.16098/j.issn.0529-1356.2019.03.018
    Abstract ( )   PDF (854KB) ( )  

    Objective To study the relationship between physiological iron reserve, anemia and elevated blood glucose in Mosuo population in Yunnan, China. Methods Blood samples were collected from 187 Mosuo people in Ninglang County, Yunnan Province, and their glycated hemoglobin (HbA1c), transferrin receptor (TfR) and hemoglobin levels (Hb) were measured. Logistic Regression analysed the relationship between iron status and elevated blood glucose in Mosuo data and Chinese national data (CHNS). Results Compared with the control group, Mosuo people were deficient in iron without anemia, ie iron deficiency erythropoiesis (IDE) corresponds to an increase in the risk of elevated blood glucose (OR=2.70), which is consistent with national data for China. The results of the analysis are consistent.  Conclusion Compared with healthy people, Mosuo people with iron deficiency anemia and non-iron deficiency anemia, Mosuo people who are deficient in iron without anemia are at risk of rising blood sugar. The body iron level of Mosuo people has reference significance for the risk and control of type 2 diabetes.

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    Circumference and its variation with age of rural adults of Salar in Qinghai
    LIANG Yu HE Yu-xiu ZHENG Lian-bin ZHANG Jing LIU Shao-qiang KANG Chen-zhe
    2019, 50 (3):  374-382.  doi: 10.16098/j.issn.0529-1356.2019.03.019
    Abstract ( )   PDF (1044KB) ( )  

    To study the circumference and its variation with age of rural adults of Qinghai salar group. Methods Using random sampling method to measure the circumference of head,chest,abdominal, hip, thigh, calf, biceps, fore-arm, and maximum biceps. The change pattern of circumference values of different age groups was analyzed; The circumference values between Salar nationality and other 17 nationalities in China with cluster analysis were compared. Results The results of variance analysis showed that the male and female circumference values of Qinghai salar had significant variation in various age groups in addition to head circumference. At the same time, the difference between the sexes in the same age group had significant variation except for thigh circumference.A linear correlation analysis showed that with the increase of age,the male circumference of chest, abdominal and hip had a linear regularity increase,and the circumference of thigh, fore-arm,and maximum biceps had a liner regularity decrease. The female circumference of chest, abdominal and hip had a linear regularity increase, and the circumference of tight hand a liner regularity decrease.In addition to the circumference of tight and hip, the other circumference values had statistically significant difference between sexes. Conclusion Circumference values of Qinghai Salar group of rural adults have the features of north type groups of our country,is close to the circumference of the Turkic ethnic groups in the Altai language of the northern ethnic group in China, but it is different from that of the ethnic minorities in southern China.

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    Body mass of the thirteen ethnic Mongolian in China
    LI Yong-lan
    2019, 50 (3):  383-391.  doi: 10.16098/j.issn.0529-1356.2019.03.020
    Abstract ( )   PDF (946KB) ( )  

    Objective To study the body mass of 13 tribal Mongolians, and to reveal the relationship between body mass and longitude, latitude, annual mean temperature and age of Mongolian. Methods Using the method of random sampling, following the principle of informed consent, the body mass of 13 ethnic Mongolian groups 4410 people(male 1917, female 2493) and the physical quality related to body mass were measured. Results The average body mass of the 13 ethnic groups in Mongolia was (57.3±8.1)kg to (81.6±12.7)kg. The 1st to 3rd places of the average body mass ranked from the largest to the smallest were the Ejina Torgouts, the Chahar of Xinjiang, and the Ordos, the 11th to 13th places were the Kazuo Mongolian, the Fuxin Mongolian, and the Yunnan Mongolian. The average body mass of women in 13 ethnic groups was between (51.6±7.6)kg to (67.7±12.6)kg. According to the average size of the body mass, the first to third places were Ejina Torgouts, Barag, and Xinjiang Torgouts, the 11th to 13th places were the Kazuo Mongolian, the Goerros, and the Yunnan Mongolian. Differences among 13 ethnic groups, age groups, and body mass were statistically significant. The fat mass values of women of all ethnic groups were greater than those of men. The fat free mass values were greater for male than female. The body mass and fat free mass of Mongolian males and females were negatively correlated with longitude and annual mean temperat re, both of which were significantly positively correlated with latitude. There was no significant correlation between male body mass and age, while females were significantly positively correlated. Fat mass of males and females was positively correlated with latitude and age, but not with longitude, and negatively correlated with annual mean temperature. Mongolian body mass was greater than the other 29 ethnic groups in China. Conclusion Mongolians have higher body mass values.

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    Cell and Molecules Biology
    Speckle-type POZ protein negatively regulating the metastasis of HepG2 cells in vitro
    LIANG Shao-hua WANG Zhi-qiang JI Pi-you
    2019, 50 (3):  304-309.  doi: 10.16098/j.issn.0529-1356.2019.03.007
    Abstract ( )   PDF (7939KB) ( )  

    Objective To explore the effect of speckle type POZ protein (SPOP) on migration and invasion of HepG2 cells in vitro. Methods Stable gain- and loss- of SPOP expression HepG2 cell lines were constructed using plasmid overexpression and shRNA technique. Western blotting was used to detect the overexpression and knockdown effect of SPOP, and the effects of SPOP on the migration and invasion of HepG2 cells was examined by Transwell assays and wound-healing assay was also used to detect HepG2 cells’ migration in vitro. Results The effect of gain- and loss- of SPOP expression was obvious in constructed stable HepG2 cells. HepG2 cells’migration and invasion were significantly decreased by gain-SPOP expression, while the cells’migration and invasion were obviously enhanced in loss-SPOP expression HepG2 cells. Wound-healing assay exhibited that SPOP overexpression suppressed HepG2 cells’ migration and that downregulation of SPOP promoted the migration of HepG2 cells. Conclusion SPOP could negatively regulate HepG2 cells’ metastasis in vitro.

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    Cancer Biology
    Bioinformatics analysis and functional analysis of bladder cancer gene microarray
    QIN Song-lin ZHOU Yang HU Wei LIU Bo-long TANG Zheng
    2019, 50 (3):  310-316.  doi: 10.16098/j.issn.0529-1356.2019.03.008
    Abstract ( )   PDF (3269KB) ( )  

    Objective To explore the pathogenesis of bladder cancer from the molecular level and provide new ideas for clinical diagnosis and prognosis evaluation. Methods Human bladder cancer-associated gene chip data GSE31189, including 52 bladder cancer samples and 40 normal bladder samples, was downloaded from a database of gene chips, and then Morpheus (https://software.broadinstitute.org/morpheus/) online tools were used to analyze cancer urothelial tissue and normal urothelial tissue. Differentially expressed genes were then analyzed online using gene-cloud of biotechnology information(GCBI) (https://www.GCBI.com.cn) for enrichment of differentially expressed gene signaling pathways and differential gene interactions. Finally, differential selection genes were selected for cell counting kit-8(CCK-8), invasion and Western blotting assays. Initially verify its function. Results According to the q value, the top 20 genes with the largest difference were selected. Among them, 18 genes were up-regulated and 2 genes were down-regulated in bladder cancer. Gene ontology(GO)analysis revealed that these differentially expressed genes are mainly involved in many biological functions such as inflammatory responses, immune responses, negative regulation of apoptosis, and transcriptional negative control from the RNA polymerase Ⅱ promoter. Pathway analysis showed that these differentially expressed genes are involved in biological processes such as transcriptional dysregulation, metabolic pathways, and nuclear factor kappa beta(NF-κB)signaling pathways in cancer. Gene network analysis found that CXC chemokine receptor 4 (CXCR4) and mitogenactivated protein kinase 10(MAPK10)are the central links in these gene networks. In vitro experiments showed that matrix metalloproteindase-12(MMP-12)downregulation led to the decreased proliferation and invasion of bladder cancer cells and the phoshorylation level of extracellular regulated protein kinases(ERK). Conclusion Multiple bioinformatics analysis can identify the key genes in bladder tumors. CXCR4 and MAPK10 are the key links in the bladder gene network. MMP-12 is highly expressed in bladder cancer cells. Downregulation of MMP-12 can inhibit the proliferation and invasion abilities of bladder cancer cells through ERK pathway.

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    Effect of dihydromyricetin on the proliferation and migration ability in choriocarcinoma JEG-3 and JAR cells
    LEI Yun-tao LIU Lei ZUO Yan-zhen XU Qian LU Yan-jie WANG Kang CUI Ying LI Yu-hong
    2019, 50 (3):  317-323.  doi: 10.16098/j.issn.0529-1356.2019.03.009
    Abstract ( )   PDF (12443KB) ( )  

    Objective To investigate the effect of dihydromyricetin(DMY)on proliferation and migration in choriocarcinoma JEG-3 and JAR cells. Methods JEG-3 and JAR cells were treated with different concentrations (0 mg/L, 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L) of DMY for 24 hours and 48 hours, and the proliferation was analyzed by methylthio tetrazole (MTT) assay. The effect of DMY on migration was detected by wound healing (after 24 hours) and Transwell assay (after treated JEG-3 for 36 hours and JAR for 24 hours). The expression of matrix metalloproteinase 2 (MMP-2) at mRNA and protein levels with different concentrations of DMY(0 mg/L, 40 mg/L, 60 mg/L, 80 mg/L) were detected by Real-time PCR (after 12 hours,24 hours,36 hours,48 hours)and Western blotting (after treated 36 hours) respectively. Results The proliferation of JEG-3 and JAR cells was inhibited significantly (P<0.05), after DMY treatment for 24 hours and 48 hours.DMY inhibited the migration of JEG-3 and JAR cells significantly (P<0.05) with a dose-dependent manner. After JEG-3 and JAR Cells treated with DMY for 36 hours and 48 hours, the expression of MMP-2 mRNA decreased significantly (P<0.05),there were no significantly changes in DMY treatment with 12 hours and 24 hours. The expression level of MMP-2 protein was inhibited significantly after treatment with DMY for 36 hours (P<0.05). Conclusion DMY might inhibit the proliferation in choriocarcinoma JEG-3 and JAR cells with a dose-dependent manner. The invasion and migration were inhibited by DMY through downregulation of MMP-2.

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    Differential expression profiles of mRNA in radioresistant non-small cell lung cancer cell line A549 model using gene expression microarray
    SUN Da-yong GUO Ya-ni ZHANG Xiang-yu WANG Tian-zhen ZUO Yan-zhen
    2019, 50 (3):  324-328.  doi: 10.16098/j.issn.0529-1356.2019.03.010
    Abstract ( )   PDF (1928KB) ( )  

    Objective To investigate the radioresistance factors in non-small cell lung cancer (NSCLC)cell line A549, and provide new targets for radiotherapy sensitization drugs development. Methods Establish the stable model of radioresistant NSCLC cell line A549 under irradiation; investigate the whole-transcriptome alteration of radioresistance cell line and radiosensitive cell line using gene expression microarray; perform bioinformatic approaches gene ontology (GO) analysis and Pathway analysis. Results The expression profile microarray showed that 1410 differentially expressed genes (733 up-regulated and 677 down-regulated) were detected in resistant and sensitive strains; GO analysis showed that it was mainly related to cell cycle and DNA replication; Pathway significant enrichment analysis showed that mitogen-activated protein kiase(MAPK) signaling pathway, phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt) signaling pathway, were mainly associated with radioresistance. Conclusion Multiple genes and signaling pathways are involved in radioresistance, further studies are needed to investigate the radioresistance factors, which could provide new targets for radiotherapy sensitization drugs development.

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     Value of four biological factors in the evaluation of neoadjuvant chemotherapy for locally advanced breast cancer
    GU Bei GUO Bin, SHI Guo-jian LI Hui-hua ZHANG Jie
    2019, 50 (3):  329-333.  doi: 10.16098/j.issn.0529-1356.2019.03.011
    Abstract ( )   PDF (4269KB) ( )  

    To explore the value of p53, Ki67, nm23 and epidermal growth factor receptor (EGFR) in the evaluation of the efficacy of neoadjuvant chemotherapy for locally advanced breast cancer. Methods From January 1, 2013 to March 31, 2018, 98 cases of locally advanced breast cancer in our hospital were selected as the research subjects. The changes of p53, Ki67, nm23 and EGFR before and after neoadjuvant chemotherapy and their relationship with clinical efficacy were detected. Results The positive expression of p53, Ki67 and EGFR after chemotherapy were all lower than those before chemotherapy, and the positive expression of nm23 was higher than that before chemotherapy, the difference was statistically significant (P<0.05). The effective rate of p53 positive was lower than that of the negative ones, and the difference was statistically significant (P<0.05). The effective rates of Ki67 and nm23 positive were higher than that of negative ones, and the difference was statistically significant (P<0.05). There was no statistical difference in effective rate between the positive EGFR and negative ones (P>0.05). Conclusion Neoadjuvant chemotherapy can significantly improve the expression of biological factors p53, Ki67, EGFR and nm23 in locally advanced breast cancer patients, and p53, Ki67 and nm23 have certain value in evaluating the curative effect of chemotherapy.

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    Neurobiology
     Silencing repulsive guidance molecule A showing protective effects on blood brain barrier after the injury of cerebral ischemia and reperfusion in rats
    LI Min FU Hong-xia ZHANG Yan-mei ZHONG Yi-liang QIN Xin-yue
    2019, 50 (3):  269-274.  doi: 10.16098/j.issn.0529-1356.2019.03.001
    Abstract ( )   PDF (4483KB) ( )  

    Objective To investigate whether silencing repulsive guidance molecule A (RGMa) shows protective effects on blood brain barrier (BBB) and tight junction protein after the injury of cerebral ischemia and reperfusion (I/R) in rats. Methods Middle cerebral artery occlusion (MCAO) /reperfusion was employed to establish the models in the male adult rats. Fourty male Sprague-Dawley rats were divided into blank control group (sh-con) and RGMa interference group (sh-RGMa). The effects of adenovirus on RGMa were observed at day 1 and day 3 after injection. The remaining 120 SD rats were randomly divided into sham group, I/R group, I/R+sh-con group and I/R+sh-RGMa group. After reperfusion for 72 hours, the neurological recovery was evaluated in rats by neurological deficit score,the infarcted volume was measured by 2,3,5-triphenyl tetrazolium chloride (TTC) staining and the permeability of BBB was performed through evans blue by tail vein injection. The expression of RGMa was detected by Western blotting and immunohistochemistry assay. The expression of claudin-5, matrix metalloprotein 9 (MMP-9)and zonula occludin 1(ZO-1)were detected by Western blotting. Results Silencing RGMa could improve the permeability of BBB, the infarcted volume, down-regulate the expression of MMP-9, and up-regulate the expression of claudin-5 and ZO-1. Conclusion Silencing RGMa shows protective effects on BBB after I/R injury in rats.

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    Mechanism of endothelin-1 mediated the cyclic intermittent hypoxia on carotid body plasticity in rats
    HUANG Lu FAN Ya-nan WANG Yang LIU Dan-hui LIU Yu-zhen
    2019, 50 (3):  275-279.  doi: 10.16098/j.issn.0529-1356.2019.03.002
    Abstract ( )   PDF (1450KB) ( )  

    Objective To investigate the potential molecular mechanism of endothelin-1 (ET-1) involved in cyclic intermittent hypoxia (CIH) induced carotid body chemoreceptor plasticity. Methods (1) Animal experiment: 32 male Sprague-Dawley (SD) rats were randomly divided into two groups: control group (Con) and CIH group (CIH), 16 rats per group. After 21 days of CIH exposure, each group (Con and CIH) was subdivided into 2 groups: tail vein injection of ET-1 (1 x 10-6mol/kg body weight) or same volume of saline according to the above dose. After 30 minutes of injection, carotid bodies were collected and Western blotting was used to detect the change of tested proteins. (2) Carotid body organ culture: rat carotid bodies were isolated and cultured in the incubator, and treated with ET-1 (1×10-4 mol/L) for different times (0 minute, 10 minutes, 60 minutes). The effect of ET-1 on the phosphorylation of p38 mitogenactivated protein kinase (p38 MAPK) was detected by Western blotting. Results (1) CIH increased the protein level of endothelin receptor A (ET-A)and ET-B in the rat carotid body. (2) Compared with the ET-1 injected Con group, phosphorylated protein kinase A (p-PKA), p-p38 MAPK, phosphorylated Ca 2+/calmodulin-dependent protein kinase Ⅱ (p-CaMKⅡ) and phosphorylated cAMP response element-binding protein (p-CREB) and RhoA protein level were significantly up-regulated in ET-1 injected CIH rats. (3) Application of ET-1 to organ cultured carotid bodies resulted in the elevation of p-p38 MAPK in a time-dependent manner. Conclusion ET-1 may regulate CIH-induced carotid body chemoreflex plasticity through PKA/p38 MAPK/CaMKⅡ/CREB and RhoA signaling.

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    Effects of microencapsulated olfactory sheathing cells transplantation on neuropathic pain and the expression of P2X7 receptor in L4-5 spinal cord
    ZHANG Wen-jun XIONG Mei-juan ZHOU Qi-bao SUN Yi LIU Zeng-xu
    2019, 50 (3):  280-284.  doi: 10.16098/j.issn.0529-1356.2019.03.003
    Abstract ( )   PDF (2128KB) ( )  

    Objective To investigate the effect of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2X7 receptor in L4-5 spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OECs group and microencapsulated OECs group (MC-OECs groups). At the 7th and 14th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method . The experssion levels of P2X7 receptor positive cells percentage and average absorbance by in situhybridization were observed in L4-5spinal cord. Results At the 7th and 14th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased(P<0.05). The percentage and average absorbance of P2X7 receptor positive cells in L4-5spinal cord significantly increased(P<0.05)compared with the CCI group, the reflex threshold of mechanical withdrawl of rats in the OECs group was significantly increased(P<0.05). The percentage and average absorbance of P2X7 receptor positive cells in L4-5 spinal cord significantly decreased compared with the OECs group(P<0.05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher(P<0.05). The percentage and average absorbance of P2X7 receptor positive cells in L4-5 spinal cord were lower(P<0.05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain better and reduce the expression levels of P2X7 receptor and repair peripheral nerve injury than OECs transplantate.

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    Effect of light intensity on expression of gonadotropin-releasing hormone in mesencephalon and diencephalon of Jinghong-Ⅰhens
    LIU Hai-bin WUZhan-fu ZHANG Li-yong GUAN Li-hui CHEN Bao-jiang HU Man
    2019, 50 (3):  285-291.  doi: 10.16098/j.issn.0529-1356.2019.03.004
    Abstract ( )   PDF (13347KB) ( )  
    Objcctive studies the effect of light intensity on expression of GnRH in Mesencephalon and Diencephalon of Jinghong-Ⅰhens. Method Jinghong-I hens aged 100d were randomly divided into four groups and applied a light of 30lx, 20lx, 10lx, and 1lx, respectively, to mensurate the distribution and expression of GnRH immunoreactive positive neurons in hen mesencephalon and diencephalon using immunohistochemical method. Results GnRH immunoreactive positive neurons were mainly observed in SGC, Imc, Ipc, SP/IPS, nbor, and SLu of mesencephalon. The mean gray values of positive neurons in 30lx and 20lx groups were significantly higher than those of 10lx and 1lx groups (p<0.05) in SGC, Imc, Ipc, and SLu. The percent cell area in SGC, Imc, and Ipc was the largest in 30 lx group, having no significant difference comparing 20lx, being significant higher than those of 10lx and 1lx (p<0.05). GnRH immunoreactive positive neurons were mainly observed in Rot, PHN, PVN, GLv, DLAm, CPa, and SM of diencephalon. The mean gray values of positive neurons in 30lx was the largest, being significantly higher than those of and 20lx, 10lx and 1lx groups (p<0.05) in Rot. There was no significant difference (p>0.05) between 30lx and 20lx group, but they were significant higher than those of 10lx and 1lx (p<0.05) in PHN, PVN, and GLv. The percent cell area in Rot was the largest in 30lx group, having no significant difference comparing 20lx, being significant higher than those of 10lx and 1lx (p<0.05). Those in 30lx and 20lx groups were significant higher than 10lx and 1lx (p<0.05) in PHN and PVN。Conclusion the expression of GnRH of 30lx and 20lx are higher significantly than 10lx and 1lx in hen mesencephalon and diencephalon.
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    Histology,Embryology and Developmental Biology
    Expression of RTCB in different tissues of mice
    HAO Hai-xia WANG Fu-long AI Gui-qing LI Ran ZHAO Na HE Cheng-qiang DING Nai-zheng
    2019, 50 (3):  346-350.  doi: 10.16098/j.issn.0529-1356.2019.03.014
    Abstract ( )   PDF (15370KB) ( )  

    Objective To investigate the expression and distribution of RTCB in various tissues of mice. Methods We detected the expression of RTCB by Western blotting and the location of the expression of RTCB in each tissue by immunohistochemistry. Results RTCB protein was all expressed in 17 tissues of mice. The relative expressions of oviduct, skeletal muscle and kidney were high, while the relative expressions of bladder and small intestine were low. In addition, the immunostaining of RTCB was different in different tissues or in different cells of the same tissue. The immunostaining was stronger in some specific cells of testis, epididymis, ovary, uterus, fallopian tube and small intestine. Conclusion RTCB may participate in the maintenance of reproductive function in mice.

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    Anatomy
    Three-dimensional morphology and asymmetry of the intraparietal sulcus in Chinese brain
    ZHANG Qi SUN Bo TANG Yu-chun LIN Xiang-tao WU Feng-xia MENG Hai-wei FENG Lei LIU Shu-wei
    2019, 50 (3):  334-339.  doi: 10.16098/j.issn.0529-1356.2019.03.012
    Abstract ( )   PDF (6870KB) ( )  

    Objective The pattern and morphological features of the intraparietal sulcus(IPS) were explored on MRI in vivo using 3D visualization method,so as to provide anatomical basis for the brain function studies of the parietal lobe as well as the diagnosis and treatment of the related diseases. Methods Totally 107 right-handed Chinese subjects were selected and scanned to acquire high quality MR images of the brains using 3.0T high-resolution magnetic resonance imaging scanner. The 3D anatomical model of the IPS was reconstructed by BrainVISA, then the morphological patterns of the IPS were observed and analyzed. Results There were three types of the IPS: continuous type, two segments type and three segments type. The gyri which separate different segments were either visible on the brain surface or buried within the sulcus. In the left brain, the continuous type was in dominant(56.07%),while the two segments type was the most common type in the right brain(58.49%),and the morphology of the IPS had significant asymmetries in both sides(P<0.01).The male brain was dominated by the two segments type(53.17%), and the female brain was dominated by the continuous type(49.43%), but it did not have significant gender asymmetry(P>0.05). Conclusion The morphology of the IPS is very complicated and it has three main types. There are obvious bilateral differences within the IPS.

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    Anatomical comparison of bilateral anterolateral thigh flap
    ZHANG Xiao-hui MENG Fan-hao ZHU Zhi-hui WANG Nai-li MA Chao CAO Cheng-gang ZHANG Tao
    2019, 50 (3):  340-345.  doi: 10.16098/j.issn.0529-1356.2019.03.013
    Abstract ( )   PDF (9523KB) ( )  

    Objective To study the anatomic variation of bilateral anterolateral thigh(ALT)flap, serving as an anatomical basis for the necessity of preoperative selection of donor thigh. Methods Ten fresh cadavers(20 cases)were recruited for the fabrication of bilateral ALT flap. The nutrient perforating branches of each flap were found out and then traced reversely to the vascular pedicle. The type and the number of perforating branches were recorded,and the length and diameter of the perforator and vascular pedicle was measured. Statistic data was expressed as x±s. Results Three cadavers were found that the bilateral ALT flap were nourished by both septocutaneous and musculocutaneous perforators, while the other 7 cadavers were found not feeded by the exactly same perforator in types bilaterally. Only 3/10 of the cadavers were noticed sharing the same number of perforator bilaterally, while the remnant 70% not. All the septocutaneous perforators pass through the intermuscular septum between the vastus lateralis and rectus femoris muscle,and all the musculocutaneous perforators penetrate vertically or obliquely into the vastus lateralis. Perforators derived solely from descending branch of lateral femoris circumflex artery(LCFA) were observed in 14 cases. The other 6 cases, 2 of which from 1 cadaver bilaterally and 4 of which from 4 cadavers unilaterally, saw that both oblique branch and descending branch of LCFA gave off perforators into the flap. The length and diameter of each main perforator and vascular pedicle in 10 cadavers bilaterally were not different signific antly(P>0.05). Conclusion Nutritive perforating branch of bilateral ALT flap differed in type, number, course and pedicle sourse,which has direct effects on the difficulty of flap fabrication and the duration of operation. Preoperative selection of donor thigh is helpful for safe and rapid preparation of anterolateral thigh flap.

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    Cell and Molecules Biology
    Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2 
    HAN Bei REN Ming-fen CHANG Yu-qiao GUO Zhi-kun
    2019, 50 (3):  292-296.  doi: 10.16098/j.issn.0529-1356.2019.03.005
    Abstract ( )   PDF (10583KB) ( )  

    Objective To detect the expression of elastin in cardiomyocytes and cardiomyocyte cell line H9C2. Methods The five fresh hearts of newborn and adult SD rats are made into frozen slices.Primary cardiomyocytes were isolated from the hearts of the twenty neonatal 3 days SD rats by the digestion of 0.05% trypsin and 0.075% type Ⅱ collagenase.The technology of immunochemical and immunofluorescence were used to detect the expression of elastin in rat myocardial tissue, primary cardiomyocytes and cardiomyocyte cell line H9C2.Elastin in supernatant of cultured cardiomyocyte cell line H9C2 was detected by ELISA. Results The co-expression of cardiac troponin T and elastin was found in rat cardiomyocytes, cardiomyocyte cell line H9C2,neonatal and adult SD rat myocardial tissues.The result of immunohistochemistry also revealed the expression of elastin in fibroblasts, smooth muscle cells, and interstitial substance.A small amount of elastin was detected in the supernatant of cardiomyocyte cell line H9C2. Conclusion Rat cardiomyocytes express elastin,it may be involved in the potential energy of myocardial cells and the construction of intercellular elastic fibers.

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    Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation
    LIU Ying LI Qiu-yan PENG Yao YU Hai-tao ZHANG Chun-jing
    2019, 50 (3):  297-303.  doi: 10.16098/j.issn.0529-1356.2019.03.006
    Abstract ( )   PDF (2827KB) ( )  

    Objective The effect of uncoupling protein 2(UCP2) on the Fadu cells of the hypopharyngeal cancer was investigated in this study. The siRNA technology was used to study the effect of UCP2 on cell cycle and cell proliferation. Meanwhile, cisplatin, a commonly chemotherapeutic drug, was researched on the effect of proliferation in Fadu cells after down-regulated UCP2 gene. Methods Flow cytometry/PI staining was used to observe the effect of UCP2 on Fadu cell cycle. Clone formation assay was used to detect the effect of down-regulated UCP2 on the proliferation of Fadu cells. MTT assay and Brud assay were used to determine whether UCP2 enhanced the sensitivity of Fadu cells to cisplatin and to detect the effect of UCP2 on the proliferation of Fadu. Results The present result showed that down-regulated UCP2 expression inhibited Fadu cell cycle in G1/S phase and cell colony formation, but had no synergistic effect on inhibition of Fadu proliferation by cisplatin; The expression of UCP2 was significantly inhibited by genipin, and then inhibited cell proliferation in a time-concentration-dependent manner. Conclusion The proliferation of Fadu cells was weakened and the cell cycle was inhibited in G1/S phase after down-regulated UCP2, suggesting that UCP2 may play an important adjust role in the development in Fadu of hypopharyngeal cancer.

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