解剖学报 ›› 2015, Vol. 46 ›› Issue (4): 548-552.doi: 10.16098/j.issn.0529-1356.2015.04.018

• 组织学胚胎学发育生物学 • 上一篇    下一篇

小鼠精子发生过程中dysbindin-1的表达

闫慧1 刘慧霞1 魏宗波2 郭蕊1 张涛1 冯雅琴1*   

  1. 1. 山西医科大学组织学胚胎学教研室,太原 030001; 2. 中国科学院遗传与发育生物学研究所,北京 100101
  • 收稿日期:2014-11-04 修回日期:2015-01-04 出版日期:2015-08-06 发布日期:2015-08-06
  • 通讯作者: 冯雅琴 E-mail:fyq1974@139.com
  • 基金资助:

    dysbindin在精子顶体形成中的作用;dysbindin在小鼠睾丸顶体形成细胞中的表达;dysbindin在精子顶体形成中的作用

Expression of dysbindin-1 during spermatogenesis

YAN Hui1 LIU Hui-xia1 WEI Zong-bo2 GUO Rui1 ZHANG Tao1 FENG Ya-qin 1*   

  1. 1. Department of Histology and Embryology, Shanxi Medical University, Taiyuan 030001,China; 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101,China
  • Received:2014-11-04 Revised:2015-01-04 Online:2015-08-06 Published:2015-08-06
  • Contact: FENG Ya-qin E-mail:fyq1974@139.com

摘要:

目的 探讨精子发生过程中dysbindin-1的表达及dysbindin-1对精子顶体形态的影响。 方法 取生后7d、14d、21d、28d、35d和3月龄的雄性小鼠各3只,用免疫印迹法检测睾丸组织dysbindin-1的表达;以dysbindin-1缺失突变的sdy小鼠为研究对象,收集附睾尾部精子,一部分制备精子涂片,HE染色显示精子形态,用异硫氰酸荧光素-豌豆凝集素(FITC-PSA)和抗精子蛋白56(sp56)单克隆抗体进行荧光染色显示顶体的结构;另一部分采用免疫印迹法检测精子中dysbindin-1的表达。
结果 不同发育阶段小鼠睾丸组织及精子中均只有dysbindin-1A,无dysbindin-1C的表达;sdy小鼠的精子及顶体的形态未见明显异常。 结论 Dysbindin-1A表达于小鼠精子发生的不同时期,dysbindin-1A对精子形态维持不起关键作用。

关键词: Dysbindin-1, 睾丸, 精子, 顶体, 免疫印迹法, sdy 小鼠, 小鼠

Abstract:

Objective To test dysbindin-1 expression during spermatogenesis and dysbindin-1 effect on histology of acrosome in mice. Methods Western blotting analysis was applied to detect the expression of dysbindin-1 in testis on day 7,14,21,28 and 35 postpartum and three-month old mice(n=3). The caudal spermatozoa was collected from sdy mice (no dysbindin-1expression), HE staining was performed for sperm morphology, fluorescein isothiocyanate conjugate-pisum sativum agglutinin (FITC-PSA) staining and immunofluorescence staining with antibody against sperm protein 56(sp56) for acrosome on sperm smear. Results Dysbindin-1A, not -1C, was expressed in postnatal testis development and spermatozoa. Mature sperm from sdy caudal epididymis had normal formation. Conclusion Dysbindin-1A, expressed in testis tissue and sperm during spermatogenesis, is not required for maintaining the morphology of sperm.

Key words: Dysbindin-1, Testis, Sperm, Acrosome, Western blotting, sdy mouse, Mouse