中药神经生长液对帕金森病模型鼠的治疗作用

doi:10.16098/j.issn.0529-1356.2016.04.002

摘要/Abstract

摘要：

目的探讨中药神经生长液对百草枯（PQ）诱导的帕金森病（PD）模型鼠的治疗作用。方法成年雄性SD大鼠40只，随机分为阴性对照组( n=10)、PQ模型组(n=10)、多巴丝肼片（美多芭）治疗组(n=10)、中药治疗组(n=10)。腹腔注射PQ制备PD模型，通过灌胃的方法对模型进行中药和美多芭治疗，以生理盐水为对照。治疗前后通过转棒实验、旷场实验和滚筒实验检测大鼠行为学变化，并记录大鼠的体重；采用酪氨酸羟化酶（TH）免疫染色检测中脑黑质多巴胺能神经元，计数并进行统计学分析。结果 1.模型制作后，与阴性对照组相比，模型动物体重显著降低（P<0.001）。然而，随着时间的延长，大鼠的体重不断增加，各组之间差异无显著性(P>0.05)。2.行为学检测结果显示，模型动物的转棒时间和滚筒时间较阴性对照组显著减少，旷场实验中的运动路程减少、静止时间延长，差异具有统计学意义(P<0.001)。灌胃1周后中药组和美多芭组在转棒实验、旷场实验和滚筒实验较模型组均有好转，差异具有统计学意义(P<0.001或P<0.01)。灌胃2周后中药组和美多芭组在转棒实验和旷场实验的路程比模型组显著增加，旷场实验中的静止时间比模型组显著减少，差异具有统计学意义(P<0.001)。3.免疫组织化学结果显示，灌胃1周时中药组TH阳性细胞数与阴性对照组相比减少（P<0.05），但与模型组相比显著增加（P<0.001），与美多芭组相比明显增加（P<0.01）；灌胃2周的中药组与模型组相比，TH阳性细胞数显著增加（P<0.001），与美多芭组相比有所增加（P<0.05）。4.免疫荧光结果显示，中药组的TH阳性细胞数和细胞形态比模型组和美多芭组要多且完整，纤维多且长，与阴性对照组差异无显著性。结论中药神经生长液对PQ诱导的PD模型鼠有一定的治疗作用。

关键词: 百草枯, 帕金森病, 多巴胺能神经元, 多巴丝肼片, 中药神经生长液, 免疫组织化学, 大鼠

Abstract:

Objective To study the therapeutic effect of the traditional Chinese medicine nerve growth decoction on the paraquat(PQ)-induced rat model of Parkinson’s disease (PD). Methods Forty adult male SD rats were randomly divided into negative control group, PQ model group, madopar group, and the traditional Chinese medicine group (n=10 in each group). PD model was generated through an intraperitoneal injection of PQ, and followed with treatment via intragastric administration traditional Chinese medicine or madopar, physiological saline as control.The animal behavior was detected through the rotarod test, open field test and drum test, and body weight was recorded before and after treatment. The numbers of dopaminergic neurons in substantia nigra were counted for statistical analysis after tyrosine hydroxylase (TH) immunohistochemical staining and immunofluorescence staining to observe the changes of dopaminergic neurons. Results 1. Compared with negative control group, body weight of animals in other three groups showed a significant reduction. However, body weight increased gradually with time lapse, and finally, there was no significant difference between groups. 2. Behavioral test indicated that the PD animals showed a decreased time in rotarod, and drum test, and the moving distance significantly reduced and static time significantly prolonged in the open field test when compared with negative control group (P<0.001). One week after intragastric administration of the traditional Chinese medicine and madopar the behavior improved significantly in rotarod test, open field test and drum experiment (P<0.001 orP<0.01). Two weeks after intragastric administration the animals in the two treatment groups showed a significant improvement in rotarod test and open field test compared with the model group (P<0.001). 3. TH immunohistochemistry showed that the number of TH positive neurons in the traditional Chinese medicine group was less than the negative control group after 1 week intragastric administration, but more than the PD model group and madopar group either 1 week or 2 weeks after intragastric administration. 4.TH immunofluorescence indicated that the TH positive cell number in the traditional Chinese medicine group was more than the PD model group and madopar group. Their morphology was more complete, and their processes were more and longer than PD model and madopar groups, but had no difference with the negative control group. Conclusion The traditional Chinese medicine nerve growth decoction has therapeutic effects on PD rats induced by PQ.

Key words: Paraquat, Parkinson’s disease, Dopaminergic neuron, Madopar, Traditional Chinese medicine nerve growth decoction, Immunohistochemistry, Rat

姜文霞张新化吕广明顾晓松. 中药神经生长液对帕金森病模型鼠的治疗作用[J]. 解剖学报, 2016, 47(4): 442-448.

JIANG Wen-xia ZHANG Xin-hua Lü Guang-ming GU Xiao-song. Effects of traditional Chinese medicine nerve growth decoction on the model of Parkinson’s disease[J]. Acta Anatomica Sinica, 2016, 47(4): 442-448.

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