早期应激对小鼠纹状体神经元发育的影响

doi:10.16098/j.issn.0529-1356.2016.04.003

解剖学报 ›› 2016, Vol. 47 ›› Issue (4): 449-455.doi: 10.16098/j.issn.0529-1356.2016.04.003

早期应激对小鼠纹状体神经元发育的影响

许本柯¹ 苗莹莹²孙安邦¹何云¹ 刘洋¹陈运才^1,3*

1. 长江大学医学院解剖学教研室，湖北荆州 434023; 2. 新乡医学院三全学院解剖学教研室，河南新乡 453003; 3. Department of Anatomg/Neurobiology, University of California, Irvine CA 92697,USA

收稿日期:2015-10-19 修回日期:2016-03-03 出版日期:2016-08-06 发布日期:2016-08-06
通讯作者: 陈运才 E-mail:yuncai_chen@hotmail.com
基金资助:
湖北省“楚天学者计划”基金

Effects of early-life stress on the development of the striatal neurons of mouse

XU Ben-ke¹ MIAO Ying-ying² SUN An-bang¹HE Yun¹ LIU Yang¹ CHEN Yun-cai ^1,3*

1. Department of Anatomy, Medical School of Yangtze University, Hubei Jingzhou 434023, China; 2. Department of Anatomy, Xinxiang Medical University, He’nan Xinxiang 453003, China; 3. Department of Anatomy/Neurobiology, University of California, Irvine CA 92697, USA

Received:2015-10-19 Revised:2016-03-03 Online:2016-08-06 Published:2016-08-06
Contact: CHEN Yun-cai E-mail:yuncai_chen@hotmail.com

摘要/Abstract

摘要：

目的探讨早期应激对背侧纹状体多棘神经元发育的影响。方法通过改变新生小鼠（出生后第2～9天）的生长环境建立早期应激动物模型，采用原位杂交、Golgi染色和体视学分析方法，定量分析应激小鼠背内侧纹状体和背外侧纹状体内神经元胞体、树突和树突棘的改变。结果 9d龄C57BL/6J小鼠的纹状体神经元含丰富的树突分支和树突棘。持续7d的应激主要影响背外侧纹状体，表现为纹状体神经元的近胞体树突分支增多（应激组9.50±0.38，n=8；对照组6.50±0.23, n=6;P<0.05），树突分支上含大量丝状伪足（每20 μm树突节段：应激组8.15±0.51，n=8；对照组3.85±0.33，n=6;P<0.05），但树突棘数量减少（每20 μm树突节段：应激组12.05±0.91，n=8；对照组20.02±0.73，n=6;P<0.05）。结论早期应激主要干扰背外侧纹状体神经元树突的发育，导致树突棘的成熟延缓。

关键词: 纹状体, 早期应激, 树突棘, 发育, 原位杂交, 小鼠

Abstract:

Objective To study the impact of early-life stress on the development of spiny neurons in the dorsal striatum. Methods The early-life stress animal model was created by changing the growth environment of new born mouse pups from postnatal day（P）2 to P 9 (P2-P9). The in situ hybridization, Golgi staining, and stereological analysis were employed to investigate the effect of stress on the soma, dendritic branches, and spines of striatal neurons. Results The striatal neurons in P9 C57BL/6J contained numerous dendritic branches and spines. Stress from P2 to P9 particularly affected the striatal neurons in the dorsolateral region, leading to abundant proximal dendritic branches (9.50±0.38 vs 6.50±0.23, n=6.8,P<0.05), and increased number of filopodia (8.15±0.51 vs 3.85±0.33 per 20 μm dendritic segment, n=6.8,P<0.05), but reduced dendritic spines (12.05±0.91 vs 20.02±0.73 per 20 μm dendritic segment, n=6.8,P<0.05). Conclusion Early-life stress interrupted the dendritic differentiation and postponed the maturation of spines of striatal neurons in the dorsolateral striatum.

Key words: Striatum, Early-life stress, Dendritic spine, Development, In situ hybridization, Mouse

许本柯苗莹莹孙安邦何云刘洋陈运才. 早期应激对小鼠纹状体神经元发育的影响[J]. 解剖学报, 2016, 47(4): 449-455.

XU Ben-ke MIAO Ying-ying SUN An-bang HE Yun LIU Yang CHEN Yun-cai. Effects of early-life stress on the development of the striatal neurons of mouse[J]. Acta Anatomica Sinica, 2016, 47(4): 449-455.

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早期应激对小鼠纹状体神经元发育的影响

Effects of early-life stress on the development of the striatal neurons of mouse

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