解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (6): 698-703.doi: 10.16098/j.issn.0529-1356.2017.06.011

• 肿瘤生物学 • 上一篇    下一篇

鳕鱼皮寡肽对人胃癌细胞SGC-7901增殖凋亡的影响及机制

吴丽萍1 续力云2 胡晓斐1 张国强1*   

  1. 1. 浙江大学舟山医院胃肠外科，浙江 舟山 316021；2. 浙江大学舟山医院分子生物学实验室，浙江 舟山 316021
  • 收稿日期:2016-10-17 修回日期:2017-08-14 出版日期:2017-12-06 发布日期:2017-12-06
  • 通讯作者: 张国强 E-mail:13615800815@126.com
  • 基金资助:
    浙江省自然科学基金项目;浙江省科技厅公益类项目

Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism

WU Li-ping1 XU Li-yun2 HU Xiao-fei1 ZHANG Guo-qiang 1*   

  1. 1. Gastrointestinal Surgery of Zhejiang University Zhoushan Hospital, Zhejiang Zhoushan 316021, China; 2. Molecular and Biology Laboratory of Zhejiang University Zhoushan Hospital, Zhejiang Zhoushan 316021, China
  • Received:2016-10-17 Revised:2017-08-14 Online:2017-12-06 Published:2017-12-06
  • Contact: ZHANG Guo-qiang E-mail:13615800815@126.com

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摘要：

目的 探讨鳕鱼皮寡肽（CSO）对人胃癌细胞（SGC-7901）的增殖影响。 方法 用不同浓度CSO处理体外培养的SGC-7901细胞后，荧光显微镜下观察细胞4’6-二脒 基-2-苯基吲哚（DAPI）染色后细胞形态变化，应用CCK-8 实验检测细胞活力，免疫印迹法和流式细胞术检测细胞凋亡及细胞周期。 结果 CSO对SGC-7901细胞增殖有明显的抑制作用，且并呈剂量、时间效应关系（P<0.05）。作用于SGC-7901胃癌细胞24 h、48 h和72 h的IC50分别是156.90 g/L、102.10 g/L、73.13 g/L。DAPI染色后发现，SGC-7901细胞随着CSO浓度增加可见大量的细胞核碎裂，荧光强烈。24h细胞凋亡率检测显示，细胞随着浓度的增加凋亡率呈上升趋势，表明CSO对SGC-7901呈浓度效应关系。细胞周期观察发现，SGC-7901细胞G1期细胞数随着CSO浓度的增加而递增，而S/G2期细胞随着浓度的细胞递降。Caspase-3、Caspase-9随着CSO浓度的逐渐增高表达上调，Bcl-2表达下调。 结论 鳕鱼皮寡肽能抑制人胃癌细胞（SGC-7901）增殖，诱导凋亡，抑癌机制可能与Caspase-3、Caspase-9上调和Bcl-2下调相关。

关键词: 鳕鱼皮寡肽, 胃癌, SGC-7901, Caspase-3, Caspase-9, Bcl-2, 免疫印迹法, 流式细胞术, 人

Abstract:

Objective To explore the effect of cod skin oligopeptide(CSO) on the proliferation in human gastric carcinoma SGC-7901 cells. Methods After treatment of SGC-7901 cells with different concentrations of CSO, the cells were observed under the microscope and the viability of cells was detected by CCK-8 assay. Cell apoptosis and cell cycle were determined by Western blotting and flow cytometry. Results CSO significantly suppressed the proliferation of SGC-7901 cells in a time-and dose-dependent manner（P<0.05）. The cultured SGC-7901 cells were treated by CSO for 24 hours and 48 hours and 72 hours with the IC50of 156.90 g/L and 102.10 g/L and 73.13 g/L.A large number of nuclear fragmentation and apoptotic bodies were observed under fluorescence microscope after treatment by DAPI. After treatment with CSO for 24 hours, SGC-7901 cells showed increased at G1 stage and cell cycle arrested at S/G2 stage, and the cell apoptosis rate increased with the CSO. With the increase of CSO concentration, the expression of Caspase-3 and Caspase-9 was up-regulated, and the expression of Bcl-2 was down-regulated. Conclusion CSO can promote the apoptosis and inhibit the proliferation of SGC-7901 cells. Anti-cancer mechanism may be related with Caspase-3 and Caspase-9 up-regulating,and Bcl-2 was down-regulating.

Key words: Cod skin oligopeptide, Gastric cancer, SGC-7901, Caspase-3, Caspase-9, Bcl-2, Western blotting, Flow cytometry, Human