解剖学报

解剖学报 ›› 2018, Vol. 49 ›› Issue (4): 524-528.doi: 10.16098/j.issn.0529-1356.2018.04.018

• 人类学 • 上一篇    下一篇

 青海省妊娠高血压综合征患者瘦素基因启动子区G-2548 A单核苷酸的多态性

庄文婷1 赵得雄2 王香林1 李红荣3 李长兴1 李福鑫1 孔得霞4 李建华1*   

  1. 1.青海大学医学院基础医学部人体解剖学教研室，西宁810001; 2.青海省红十字医院妇产科，西宁810001; 3.甘肃省武威肿瘤医院外科，甘肃 武威733000;4.西宁市第二人民医院产科，西宁810001
  • 收稿日期:2018-01-05 修回日期:2018-03-10 出版日期:2018-08-06 发布日期:2018-08-06
  • 通讯作者: 李建华 E-mail:1184666039@qq.com
  • 基金资助:
    多因子基因多态性与青海藏族、汉族妊娠高血压疾病相关性研究

Polymorphism of leptin gene G-2548 A and pregnancy-induced hypertension syndrome in Han population of Qinghai Province

ZHUANG Wen-ting1 ZHAO De-xiong2 WANG Xiang-lin1 LI Hong-rong3 LI Chang-xing1 LI Fu-xin1 KONG De-xia4 LI Jian-hua 1*   

  1. 1.Department of Human Anatomy，Basic Medicine Science, Medical College of Qinghai University, Xining810001, China; 2.Department of Gynecology, Qinghai Red Cross Hospital, Xining810001, China; 3.Department of Surgical，Gansu Wuwei Tumor Hospital, Gansu Wuwei733000, China ; 4. Department of Gynecology, the Second People’s Hospital of Xining City，Xining810001,China
  • Received:2018-01-05 Revised:2018-03-10 Online:2018-08-06 Published:2018-08-06
  • Contact: LI Jian-hua E-mail:1184666039@qq.com
  • Supported by:
    Relationship between multifactorial gene polymorphism and gestational hypertension in Tibetan and Han nationalities in Qinghai

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摘要：

目的  探讨青海省妊娠高血压综合征(PIH)与瘦素基因启动子区单核苷酸多态性的关系。方法  选择青海省PIH患者130例，正常妊娠对照组135例，应用聚合酶链反应限制性内切酶片段长度多态性(PCR-RFLP)方法，分析PIH患者和对照组瘦素基因G2548 A多态性分型并测序验证。结果  PIH组基因型频率GG型、GA型和AA型分别为53.1%、40%和6.9%，对照组分别为60%、34.1%和5.9%。PIH组和对照组瘦素基因 G-2548 A 位点等位基因A和G频率分布具有差异性（P<0.05），妊娠高血压综合征组G等位基因频率高于对照组(χ2=4.21，P<0.05，OR =0.66，95%CI=0.45~0.99)。结论  青海省瘦素基因G2548位点G/A基因多态性与其妊娠高血压的易感性有关。G等位基因可能为妊娠高血压综合征的易感基因，A等位基因可能为妊娠高血压综合征的保护基因。

关键词: 青海, 妊娠高血压综合征, 瘦素, 基因多态性, 基因频率, 聚合酶链反应-限制性内切酶片段长度多态性, 孕妇

Abstract:

Objective To investigate the relationship between polymorphism of leptin gene promoter region G-2548 A and pregnancy-induced hypertension syndrome（PIH）in Han population of Qinghai Province. Methods  A total of 130 PIH subjects and 135 normal pregnancy subjects were studied. The genotype of leptin was analyzed by polymerase chain reaction fragment length polymorphism (PCR-RFLP). The mutation was confirmed by sequencing. Results  The frequencies of leptin GG, GA, and AA genotype in the PIH group were 53.1%, 40% and 6.9%, and in the control group were 60%, 34.1% and 5.9% respectively. There was difference in frequency distribution of leptin genotype between the PIH and control groups. The frequency of G gene in the PIH group was higher than the control group (χ2=4.21，P<0.05，OR=0.66，95%CI=0.45-0.98). Conclusion  Leptin gene G-2548 A is significantly correlated with PIH in Han group of QingHai. G allele may be a susceptible gene to PIH (OR=1.361, 95%CI: 1.01-1.83) and A allele may be a protective gene to PIH (OR=0.901, 95%CI: 0.82-1.00).

Key words: Qinghai Province, Pregnancy-induced hypertension syndrome, Leptin, Gene polymorphism, Gene frequency, PCR-RFLP, Pregnant woman