血管紧张素转换酶和血管紧张素原基因多态性及其与青海妊娠高血压疾病的相关性

doi:10.16098/j.issn.0529-1356.2020.02.024

解剖学报 ›› 2020, Vol. 51 ›› Issue (2): 294-299.doi: 10.16098/j.issn.0529-1356.2020.02.024

血管紧张素转换酶和血管紧张素原基因多态性及其与青海妊娠高血压疾病的相关性

王茹¹ 庄文婷¹王香林² 李红荣³ 李长兴¹孔德霞⁴ 李建华^1*

1.青海大学医学院基础医学部人体解剖学教研室，西宁 810001； 2.广州市花都区人民医院放射科，广州 510800；3. 甘肃省武威肿瘤医院外科，甘肃武威 733000; 4. 西宁市第二人民医院产科，西宁 810001

收稿日期:2019-05-06 修回日期:2019-06-17 出版日期:2020-04-06 发布日期:2020-04-06
通讯作者: 李建华 E-mail:1184666039@qq.com
基金资助:
青海省科技厅计划项目;青海省科技厅计划项目

Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai

WANG Ru¹ ZHUANG Wen-ting¹WANG Xiang-lin² LI Hong-rong³ LI Chang-xing¹ KONG De-xia⁴ LI Jian-hua^1*

1.Department of Human Anatomy，Basic Medicine Science，Medical College of Qinghai University，Xining 810001，China; 2.Department of Radiology,Huadu District People’s Hospital,Guangzhou 510800,China; 3.Department of Surgical，Gansu Wuwei Tumor Hospital，Gansu Wuwei 733000，China; 4.Department of Gynecology,the Second People，s Hospital of Xining City，Xining 810001，China

Received:2019-05-06 Revised:2019-06-17 Online:2020-04-06 Published:2020-04-06
Contact: LI Jian-hua E-mail:1184666039@qq.com

摘要/Abstract

摘要：

目的探讨血管紧张素转换酶(ACE)和血管紧张素原 (AGT) 基因表达、基因多态性与青海孕产妇妊娠高血压疾病的相关性。方法选择210例妊娠高血压患者（HDCP组）和220例正常孕妇（CK组），运用限制性内切酶片段长度多态性聚合酶链反应(PCR-RFLP)方法检测AGT M235T、ACE I/D 基因多态性。结果 CK组ACE基因 DD、ID、Ⅱ 所占比例分别为 28.18%、47.73%、24.09%,HDCP组分别为33.81%、51.90%、14.29%（P<0.05，故两组的ACE基因分布有差异），HDCP组和对照组ACE I/D多态性等位基因I和D频率分布有差异（P<0.05,HDCP组D等位基因频率高于对照组（χ2=5.188,P<0.05），ACE基因型分布符合Hardy-Weinberg遗传平衡（χ2=0.423，df=2,查表χ2 界值表，P＞0.05，达到遗传平衡）；CK组AGT基因 MM、MT、TT 所占比例分别为 24.09%、43.64%、32.27%，HDCP组分别为15.71%、42.86%、41.43%（P<0.05,故两组的AGT基因分布差异有统计学意义），HDCP组和对照组AGT M235T多态性等位基因M和T频率分布有差异性（P<0.05），HDCP组T等位基因频率高于对照组（χ2＝6.796，P<0.05），AGT基因型分布符合Hardy-Weinberg遗传平衡（χ2＝3.242，df=2，查表χ2界值表，P＞0.05，达到遗传平衡）。结论 ACE I/D多态性和AGT M235T多态性与青海省汉族妊娠期高血压疾病有关，D等位基因和T等位基因可能是妊娠高血压疾病的易感基因。

关键词: 青海, 血管紧张素转换酶, 血管紧张素原, 妊娠高血压, 基因多态性, 限制性内切酶片段长度多态性聚合酶链反应, 孕妇

Abstract:

Objective To investigate the relationship between angiotensin converting enzyme (ACE) and angiotensinogen (AGT) gene expression, gene polymorphism and pregnancy-induced hypertension in Qinghai. Methods A total of 210 pregnant hypertensive patients (HDCP group) and 220 normal pregnant women (CK group) were enrolled. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect AGT M235T and ACE I/D gene polymorphism. Results The proportions of ACE gene DD, ID, and Ⅱ in CK group were 28.15%, 47.73%, and 24.09%, respectively. The HDCP group was 33.81%, 51.90%, and 14.29%, respectively (P<0.05). The frequency distribution of ACE I/D polymorphic alleles I and D was different between HDCP group and CK group(P<0.05). D allele frequency was higher in HDCP group than in CK group (χ2=5.188, P<0.05). ACE genotype distribution was accorded with Hardy-Weinberg genetic balance(χ2=0.423, df=2, check table χ2 boundary value table, P>0.05, reach genetic balance). The proportions of MM, MT and TT in CK group was 24.09%, 43.64% and 32.27%, respectively. The HDCP group was 15.71%, 42.86%, and 41.43% respectively (P<0.05). HDCP group and CK group AGT M235T polymorphic alleles M and T frequency distribution were different (P<0.05). HDCP group T allele frequency was higher than the CK group(χ2=6.796, P<0.05). AGT gene were genotype distribution accorded with Hardy-Weinberg genetic balance (χ2=3.242, df=2, check table χ2 boundary value table, P>0.05, reaching genetic balance). Conclusion The ACE I/D polymorphism and AGT M235T polymorphism are associated with hypertensive disorder of pregnancy in Han nationality in Qinghai Province. D allele and T allele may be susceptible genes of pregnancy-induced hypertension.

Key words: Qinghai, Angiotensin-converting enzyme, Angiotensinogen, Pregnancy-induced hypertension, Gene polymorphism, Polymerase chain reaction-restriction fragment length polymorphism, Pregnant woman

中图分类号:

王茹庄文婷王香林李红荣李长兴孔德霞李建华. 血管紧张素转换酶和血管紧张素原基因多态性及其与青海妊娠高血压疾病的相关性[J]. 解剖学报, 2020, 51(2): 294-299.

WANG Ru ZHUANG Wen-ting WANG Xiang-lin LI Hong-rong LI Chang-xing KONG De-xia LI Jian-hua. Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai[J]. Acta Anatomica Sinica, 2020, 51(2): 294-299.

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血管紧张素转换酶和血管紧张素原基因多态性及其与青海妊娠高血压疾病的相关性

Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai

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