解剖学报

解剖学报 ›› 2019, Vol. 50 ›› Issue (5): 565-569.doi: 10.16098/j.issn.0529-1356.2019.05.004

• 神经生物学 • 上一篇    下一篇

鞘内注射M_ERK2-shRNA腺病毒对糖尿病神经痛小鼠的影响

王月静1 姚宏波1 张萌1 廉洁1 张海燕1 刘丹阳1 李林1 张宇华2 李尽贺2 孙丽慧1*   

  1. 1.齐齐哈尔医学院组织学胚胎学教研室;2.齐齐哈尔市第一医院普外科，黑龙江 齐齐哈尔 161001
  • 收稿日期:2018-11-08 修回日期:2019-01-09 出版日期:2019-10-06 发布日期:2019-12-10
  • 通讯作者: 孙丽慧 E-mail:335187646@qq.com
  • 基金资助:
    齐齐哈尔市科学技术计划项目

Effects of intrathecal injection of M_ERK2-shRNA adenovirus on diabetic neuropathic pain in mouse

WANG Yue-jing1 YAO Hong-bo1 ZHANG Meng1 LIAN Jie1 ZHANG Hai-yan1 LIU Dan-yang1 LI Lin1 ZHANG Yu-hua2 LI Jin-he2 SUN Li-hui 1*   

  1. 1.Department of Histology and Embryology，Qiqihar Medical College，Heilongjiang Qiqihar 161001，China; 2.Department of General Surgery，Qiqihar First Hospital，Heilongjiang Qiqihar 161001，China
  • Received:2018-11-08 Revised:2019-01-09 Online:2019-10-06 Published:2019-12-10
  • Contact: SUN Li-hui E-mail:335187646@qq.com

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摘要：

目的 探讨细胞外调节蛋白激酶2 (ERK2）在糖尿病神经病理性疼痛（DNP）小鼠模型中的作用机制。方法40只小鼠随机分4组：对照组，DNP组，siERK2-DNP组（鞘内注射M_ERK2-shRNA腺病毒），siRNA-DNP组（鞘内注射siRNA腺病毒）。模型制作成功后检测痛阈值；模型制作前、模型制作后2周、 4周及6周检测机械痛阈值；6周后取脊髓组织，利用Western blotting及免疫组织化学法检测脊髓后角ERK2及核因子κB (NFκB)的表达。 结果 与对照组比较，DNP组和siRNA-DNP组痛阈值显著降低， ERK2、NFκB的表达显著上调（P<0.05），siERK2-DNP组与对照组比较，痛阈值无显著变化， ERK2、NFκB的表达无显著差别（P>0.05）。 结论 下调ERK2可能是治疗糖尿病神经病理性疼痛的脊髓后角神经元损伤的方法之一。

关键词: siERK2, 糖尿病, 神经病理性疼痛, 脊髓后角神经元, 核因子κB, 免疫印迹法, 小鼠

Abstract:

Objective To explore the mechanism of extracellular regulated protein kinase 2 (ERK2) in diabetic neuropathic pain(DNP) mice. Methods Forty mice were randomly divided into four groups: control group, DNP group, siERK2-DNP group (intrathecal injection of M_ERK2-shRNA adenovirus), siRNA-DNP group (intrathecal injection of siRNA adenovirus). The pain threshold was detected after the model made successfully, the mechanical pain threshold was detected before moulding, after moulding 2 weeks, 4 weeks and 6 weeks. Spinal cord tissues were taken after 6 weeks, and the expression of ERK2 and nuclear factor κB (NFκB) in the posterior horn of the spinal cord was detected by Western blotting and immunohistochemistry. Results Compared with the control group, the pain threshold of DNP group and siRNA-DNP group decreased significantly, the expression of ERK2 and NFκB increased significantly (P<0.05). Compared with the control group, the pain threshold of siERK2-DNP group had no significant change, and the expression of ERK2 and NFκB had no significant difference (P>0.05). Conclusion The marked down-regulation of ERK2 may be one of the important mechanisms affecting neuronal injury in the spinal dorsal horn of diabetic neuropathic pain.

Key words: siERK2, Diabetes, Neuropathic pain, Spinal dorsal horn neurons, Nuclear factor κB, Western blotting, Mouse