解剖学报

解剖学报 ›› 2020, Vol. 51 ›› Issue (5): 688-692.doi: 10.16098/j.issn.0529-1356.2020.05.009

• 解剖学与耳鼻喉科学 • 上一篇    下一篇

CXCL10在嗜酸性粒细胞型与非嗜酸性粒细胞型慢性鼻-鼻窦炎伴鼻息肉的差异表达及意义#br#

闫冰1,2,3 刘畅1,2,3 王阳1,2,3 王成硕1* 张罗1,2,3*   

  1. 1.首都医科大学附属北京同仁医院耳鼻咽喉头颈外科，耳鼻咽喉头颈科学教育部重点实验室，北京 100730; 2.北京市耳鼻咽喉科研究所，鼻病研究北京市重点实验室，北京 100005; 3.首都医科大学附属北京同仁医院变态反应科，北京 100730
  • 收稿日期:2020-04-09 修回日期:2020-05-26 出版日期:2020-10-06 发布日期:2020-10-06
  • 通讯作者: 王成硕;张罗 E-mail:dr.luozhang@139.com
  • 基金资助:
    北京市自然科学基金;北京市医管局科研培育计划;北京市属医学科研院所公益发展改革试点项目

Differential expression and significance of CXCL10 in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps#br#

YAN Bing1,2,3 LIU Chang1,2,3 WANG Yang1,2,3 WANG Cheng-shuo1* ZHANG Luo1,2,3*   

  1. 1.Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical University, Beijing 100730, China;   2.Beijing Institute of Otorhinolaryngology, Beijing Key Laboratory of Nasal diseases, Beijing 100005, China; 3.Department of Allergology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
  • Received:2020-04-09 Revised:2020-05-26 Online:2020-10-06 Published:2020-10-06
  • Contact: Cheng-shuo;ZHANG Luo E-mail:dr.luozhang@139.com

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摘要：

目的 探讨CXC趋化因子配体10（CXCL10）在嗜酸性粒细胞型及非嗜酸性粒细胞型慢性鼻-鼻窦炎伴鼻息肉的表达及意义。 方法 采用回顾性研究方法，依据欧洲慢性鼻窦炎及鼻息肉共识（EPOS2012），纳入诊断为慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）的患者共35名，术中收集鼻息肉标本。采用Real-time PCR方法检测鼻息肉内CXCL10含量；采用HE染色法对病理活检标本染色，计算嗜酸性粒细胞、中性粒细胞、浆细胞、淋巴细胞百分比，并依据比值将CRSwNP分为嗜酸性粒细胞型（ECRSwNP）与非嗜酸性粒细胞型（nonECRSwNP）；采用Spearman方法分析CXCL10水平与息肉组织中炎细胞百分比的相关性；采用受试者工作特征（ROC）曲线评估CXCL10预测nonECRSwNP的可靠性。  结果 nonECRSwNP中CXCL10水平显著高于ECRSwNP组（P<0.05）。鼻息肉CXCL10 mRNA水平与息肉组织嗜酸性粒细胞百分比成显著负相关（P<0.05, r=－0.395），与组织浆细胞成显著正相关（P<0.05,r=0.389）。ROC曲线及约登指数表明，鼻息肉组织中CXCL10预测nonECRSwNP的最佳界值为0.026（以GAPDH为内参基因），其敏感度为58.33％，特异性为100％（曲线下面积=0.761，P<0.01）。  结论 非嗜酸性粒细胞型慢性鼻-鼻窦炎伴鼻息肉中CXCL10表达高于嗜酸性粒细胞型慢性鼻-鼻窦炎伴鼻息肉，提示CXCL10可能作为生物学标志物预测非嗜酸性粒细胞型慢性鼻-鼻窦炎伴鼻息肉。

关键词:  CXC趋化因子配体10, 生物标志物, 慢性鼻-鼻窦炎伴鼻息肉, 嗜酸性粒细胞, 实时定量聚合酶链反应, 人

Abstract:

 Objective To evaluate the expression and significance of C-X-C motif chemokine ligand 10 (CXCL10) in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps.   Methods A retrospective analysis, with thirty-five adult CRSwNP patients enrolled in this study. Nasal biopsy was performed and tissue sample was stored at RNA later reagent. Real-time PCR assay was used to investigate the CXCL10 level in polyp tissues. HE staining was performed for histological assessment of CRSwNP. CRSwNP was divided into eosinophilic CRSwNP (ECRSwNP) and non-eosinophilic CRSwNP (nonECRSwNP) based on the percentage of infiltrated eosinophils. Spearman rank analysis was used to measure the correlation between the CXCL10 level and the percentage of inflammatory cells. The receiver operating characteristic (ROC) curve was used to assess the predictive value of the CXCL10 level.  Results The CXCL10 level in nasal tissue is significantly higher in nonECRSwNP than in ECRSwNP samples (P<0.05). The CXCL10 mRNA level was negatively correlated with the percentage of tissue eosinophils (P<0.05, r=-0.395) whereas positively correlated with the percentage of tissue plasma cells (P<0.05, r=0.389). The ROC curve and Youden index indicated that the optimal cut-off value of 0.026 for CXCL10 normalized to GAPDH (the housekeeping gene) could predict nonECRSwNP with 58.33% sensitivity and 100% specificity (area under the curve=0.76, P<0.01).   Conclusion The CXCL10 level in nasal tissue is significantly higher in nonECRSwNP than in ECRSwNP, indicating the CXCL10 level in nasal polyp tissue may serve as a biomarker to predict nonECRSwNP.

Key words: C-X-C motif chemokine ligand 10, Biomarker, Chronic rhinosinusitis with nasal polyps, Eosinophil, Real-time PCR, Human

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