解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (1): 73-77.doi: 10.16098/j.issn.0529-1356.2021.01.011

• 肿瘤生物学 • 上一篇    下一篇

微小RNA-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1调节宫颈癌细胞迁移和侵袭的机制

王丽娜1 刘永娟1 张莹1 刘荣霞1 梁珊1 宋春红2 崔娜3*   

  1. 1. 河北省石家庄市第四医院妇科，石家庄050011; 2.河北省石家庄市第四医院病理科，石家庄050011; 3. 河北医科大学第二医院妇产科，石家庄050005
  • 收稿日期:2020-05-23 修回日期:2020-08-04 出版日期:2021-02-06 发布日期:2021-02-06
  • 通讯作者: 崔娜 E-mail:dyyzxj01@163.com
  • 基金资助:
    河北省自然科学基金项目

MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer  cell migration and invasion

WANG Li-na1  LIU Yong-juan1  ZHANG Ying1  LIU Rong-xia1  LIANG Shan1  SONG Chun-hong2  CUI Na3*   

  1. 1. Department of Gynecology, the Fourth Hospital of Shijiazhuang City, Shijiazhuang050011, China; 2. Department of Pathology, the Fourth hospital of Shijiazhuang City, Shijiazhuang050011, China; 3. Department of Obstetrics, the Second Hospital of Hebei Medical University, Shijiazhuang050005, China
  • Received:2020-05-23 Revised:2020-08-04 Online:2021-02-06 Published:2021-02-06
  • Contact: CUI Na E-mail:dyyzxj01@163.com

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摘要：

目的  探讨微小RNA（miR)-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1（HDAC1）调节宫颈癌细胞迁移和侵袭的机制。 方法  临床收集宫颈癌患者86例，通过Real-time PCR检测肿瘤组织和癌旁组织中miR-513c-5p水平，分析其与宫颈癌病理特征的关系。通过双荧光素酶报告验证miR-513c-5p靶向HDAC1。将宫颈癌HeLa细胞系分为4组：对照组、类似物(mimic)组、mimic+HDAC1组和HDAC1组。通过质粒转染技术过表达miR-513c-5p和(或)HDAC1。Real-time PCR和Western blotting分别用于检测RNA或蛋白的表达水平。分别通过CCK-8法、细胞划痕实验和Transwell实验检测各组的细胞生长、迁移和侵袭能力。 结果  宫颈癌组织中miR-513c-5p水平显著低于癌旁组织。低水平的miR-513c-5p与更高的局部侵袭、淋巴转移和远端转移有关（P<0.05）。miR-513-5p靶向抑制HDAC1表达。过表达miR-513c-5p显著抑制宫颈癌细胞生长、迁移和侵袭（P<0.05）。过表达HDAC1促进细胞生长、迁移和侵袭（P<0.05），并且可以逆转miR-513c-5p的抑制作用（P<0.05）。  结论  低水平的miR-513c-5p可能与宫颈癌转移有关，并且miR-513c-5p可通过靶向抑制HDAC1蛋白的表达抑制宫颈癌HeLa细胞生长、迁移和侵袭。

关键词: 宫颈癌, 微小RNA-513c-5p, 组蛋白去乙酰化酶1, 迁移, 侵袭, 双荧光素酶报告, 实时定量聚合酶链反应, 免疫印迹法, 人

Abstract:

Objective  To investigate the expression  of microRNA (miR)-513c-5p in cervical cancer and the mechanism  of targeting histone deacetylase 1 (HDAC1) regulating cervical cancer cell migration and invasion.   Methods  Clinically collected 86 patients with cervical cancer. The levels of miR-513c-5p in tumor tissues and adjacent tissues were detected by Real-time PCR. The relationship between miR-513c-5p and pathological characteristics of cervical cancer was analyzed. It was verified that miR-513c-5p targets HDAC1 by a dual luciferase report. Cervical cancer HeLa cells were divided into four groups: control group, mimic group, mimic+HDAC1 group and HDAC1 group. MiR-513c-5p and(or) HDAC1 were overexpressed by plasmid transfection technology. Real-time PCR and Western blotting were used to detect the expression level of RNA or protein, respectively. The cell growth, migration, and invasion capabilities of each group were measured by CCK-8 method , cell scratch test, and Transwell test.   Results  The level of miR-513c-5p in cervical cancer tissues was significantly lower than that in adjacent tissues. Low levels of miR-513c-5p were associated with higher local invasion, lymphatic metastasis, and distal metastasis (P<0.05). MiR-513-5p targeted HDAC1 expression. Overexpression of miR-513c-5p inhibited significantly the growth, migration and invasion of cervical cancer cells (P<0.05). Overexpression of HDAC1 promoted growth, migration and invasion (P<0.05), and reversed the inhibitory effect of miR-513c-5p (P<0.05).   Conclusion  Low levels of miR-513c-5p might be related to cervical cancer metastasis, and miR-513c-5p could inhibit the growth, migration and invasion of cervical cancer HeLa cells by targeted inhibition of HDAC1 protein expression.

Key words: Cervical cancer, MicroRNA-513c-5p, Histone deacetylase 1, Migration, Invasion, Dual luciferase report, Real-time PCR, Western blotting, Human

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