解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (5): 605-609.doi: 10.3969/j.issn.0529-1356.2014.05.004

• 神经生物学 • 上一篇    下一篇

两种帕金森病大鼠糖脂代谢模型的比较

孟祥志1 郑瑞攀1 张迎1 乔明亮2 景鹏1 高艳1*   

  1. 1. 首都医科大学人体解剖学与组织学胚胎学系，北京 100069; 2. 山东省荣成市石岛人民医院神经外科， 山东 荣成 264300
  • 收稿日期:2014-03-13 修回日期:2014-04-28 出版日期:2014-10-06 发布日期:2013-10-06
  • 通讯作者: 高艳 E-mail:gy1003@ccmu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目

Comparison of glucose and lipid metabolism in two types of Parkinson’s disease rat models

MENG Xiang-zhi1 ZHENG Rui-pan1 ZHANG Ying1 QIAO Ming-liang2 JING Peng1 GAO Yan 1*   

  1. 1. Department of Anatomy, Histology and Embryology, Capital Medical University, Beijing 100069, China;2.Department of Neurosurgery, Rongcheng Shidao People’s Hospital of Shandong Province, Shandong Rongcheng 264300, China
     
  • Received:2014-03-13 Revised:2014-04-28 Online:2014-10-06 Published:2013-10-06
  • Contact: Yan GAO E-mail:gy1003@ccmu.edu.cn

摘要：

目的 筛选合并代谢异常的帕金森病模型，为后续的相关实验研究提供理想的动物模型。方法 SD雄性大鼠62只，随机分为损毁前脑内侧束（MFB）模型组、损毁MFB假手术组、损毁纹状体模型组和损毁纹状体假手术组。模型制做成功后进行旋转实验，两组大鼠中分别选出成功模型，并分别对两组成功的PD模型大鼠的摄食、体重、血糖和腹腔内脂肪组织进行检测。观察两组模型大鼠上述代谢指标的变化。 结果 损毁MFB较损毁纹状体制作帕金森病模型的成功率高(70.97% 比38.71%)。与各假手术组大鼠相比，MFB模型大鼠既出现体重明显的减轻［(218.1±13.99)g 比（252.7±10.1）g， P<0.05］，腹腔注射葡萄糖后15min和30min血糖出现了明显升高，并且肾周白色脂肪组织明显减少(左右两侧)。纹状体模型大鼠仅饮食减少［(13.95±0.25)g 比（20.23±0.86）g，P<0.001］和腹腔注射葡萄糖后15min、30min和60min时间点血糖升高。 结论 损毁MFB的PD模型大鼠无论模型成功率还是代谢相关指标都较损毁纹状体的PD模型更接近糖脂代谢异常的PD患者。

关键词: 帕金森病, 糖脂代谢, 6羟多巴胺, 免疫组织化学, 大鼠

Abstract:

Objective To select an ideal Parkinson’s disease (PD) animal model with metabolic abnormalities for subsequent experimental studies. Methods A total of 62 Sprague-Dawley male rats were randomly divided into four groups: damaged medial forebrain bundle (MFB) model group, damaged medial forebrain bundle (MFB) sham group, damaged Striatum model group and damaged Striatum sham group. After detecting the rotation experiment, successful model rats of two groups were selected to detect the changes of food intake, body weight, blood glucose and intra-abdominal adipose tissue. Results It was easier to produce a PD model by destroying MFB than striatum. Compared with sham-operated rats, MFB model rats showed significant abnormality both in reduction of body weight ［(218.1±13.99)g vs (252.7±10.1)g, P<0.05］ and high blood glucose appeared at 15min and 30min after introperitoneal glucose tolerance test (IPGTT). Their perirenal white adipose tissue was significantly reduced (both left and right side). Striatum model rats only appeared decreased food intake ［(13.95±0.25)g vs (20.23±0.86)g, P<0.001］ and impaired glucose regulation at 15min, 30min and 60min after IPGTT. Their body weight and adipose tissue did not change significantly. Conclusion No matter in the success rate or metabolismrelated indicators, MFB damaged rat model of PD is more suitable to study PD patients with abnormal lipid metabolism compared with Striatum rat model.

Key words: Parkinson disease, Glucose and lipid metabolism, 6-hydroxydopamine, Immunohistochemistry, Rat