›› 2007, Vol. 38 ›› Issue (1): 70-74.doi:
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王海萍;张雷*;李秀华
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关键词: 骨髓间充质干细胞, 5-氮胞苷, 心肌细胞, 激光扫描共焦显微镜, 大鼠
Abstract: Objective To establish a new cell resource of stem cell transplantation for heart failure therapy, by using cardiomyocytes from rat marrow mesenchymal stem cells(MSCs). Methods MSCs were isolated and cultured from rat bone marrow, then induced by 5-aza (5, 10, 15, 20 μmol/L) for 24 hours. The cultured cells were observed with phasecontrast microscopy. The immunohistochemical technique and laser scanning confocal microscopy (LSCM) were applied for revealing the expressions of desmin, α-sarcomeric actin and C-TnT. The induced cells were evaluated by a transmission electron microscope. GATA4 and α-MHC expressions were detected by relative quantitative RT-PCR after 7, 21, 28 days of inducement respectively. Results MSCs induced in vitro by 5-aza could be identified by the positive staining for desmin, α-sarcomeric actin and C-TnT. The number of cells stained positively in the 5 μmol/L was greater than that of other groups. Transmission electron microscope showed that paralleled myofilaments were formed. RT-PCR assessment showed that the differentiated cells began to express GATA4 from day 7 to day 28 of differentiation and began to express αMHC from day 21 to day 28 of differenti
Key words: Bone marrow mesenchymal stem cells, 5-azacytidine, Cardiomyocytelike cells, Cell differentiation, Laser scanning confocal microscopy, Rat
中图分类号:
R331.31
王海萍;张雷;李秀华. 大鼠骨髓间充质干细胞体外诱导分化为心肌样细胞 P class=MsoNormal><SPAN style=/P>[J]. , 2007, 38(1): 70-74.
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