解剖学报

›› 2010, Vol. 41 ›› Issue (2): 185-190.doi: 10.3969/j.issn.0529.1356.2010.02.004

• 论著 • 上一篇    下一篇

17-β雌二醇提高巯醇抗氧化物（酶）治疗脊髓损伤的作用 

边立功1;刘承杏１;李兴国1;马爱斌2 ;邹智荣1 ;李守民1 ;孙俊1*; 陆地1*    

  1. 1.昆明医学院人体解剖学教研室, 昆明 650031; 2.昆明医学院第一附属医院神经内科, 昆明 650032
  • 收稿日期:2009-02-02 修回日期:2009-03-26 出版日期:2010-04-06
  • 通讯作者: 孙俊;陆地

17-β estradiol reduces spinal cord injury of rats through raising thiol antioxidants

  1. 1.Department of Anatomy, Kunming Medical University, Kunming 650031, China;2.Department of Neurology, the First Affiliated Hospital, Kunming Medical University, Kunming 650032, China
  • Received:2009-02-02 Revised:2009-03-26 Online:2010-04-06
  • Contact: sunjun91@126.com;SUN Jun;LU Di

关键词: 17-β雌二醇, 脊髓损伤, 巯醇抗氧化物酶, 凋亡相关蛋白, 化学比色法, 免疫组织化学, 大鼠

Abstract: Objective To examine the protective effects of 17-β estradiol on the experimental model of spinal cord injury (SCI) rats. Methods One hundred and eighty male Sprague Dawley (SD) rats, after Allen’s model, SD rats were divided into three groups: the sham group, the acute spinal cord injury (control groups) and the acute spinal cord injury supplying with 17-β estradiol treatment group. SCI was made by Allen’s weight dropping, impacting on the posteriors of spinal cord T10.The content of malonyldialdehyed (MDA), glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined by chromatometry. The expressions of Caspase-3 and Bcl-2 family in the injured spinal cord were detected by immunohistochemical staining. Results The BBB scores at each time point in 17-β estradiol treatment group were significantly higher than that in SCI group (EM>P/EM><0.05). The contents of GSH, SOD, GSH-Px and the expression of Bcl-2 protein at the majority of time point in 17-β estradiol treatment group were significantly higher than that in SCI group(EM>P/EM><0.05), however, the MDA, Caspase-3 and Bax were markedly decreased (EM>P/EM><0.05). Conclusions This study suggests that 17-β estradiol administration

Key words: 17-β estradiol, Spinal cord injury, Thiol antioxidant, Apoptotic protein, Chromatometry, Immunohistochemistry, Rat

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