解剖学报 ›› 2015, Vol. 46 ›› Issue (2): 170-174.doi: 10.16098/j.issn.0529-1356.2015.02.005

• 神经生物学 • 上一篇    下一篇

痛抑郁二联征模型大鼠中缝背核不同水平5羟色胺表达差异

吴媛媛 蒋永亮 邵晓梅 赵晓芸 何晓芬 方剑乔*   

  1. 浙江中医药大学第三临床医学院针灸神经生物学实验室,杭州 310053
  • 收稿日期:2014-11-13 修回日期:2015-01-13 出版日期:2015-04-06 发布日期:2015-04-06
  • 通讯作者: 方剑乔 E-mail:fangjianqiao7532@163.com
  • 基金资助:

    国家自然科学基金资助项目;浙江省省自然科学基金资助项目;浙江省省自然科学基金资助项目

Comparisons of 5-hydroxytryptamine expression at different levels of dorsal raphe nucleus in rats with pain-depression dyad

WU Yuan-yuan JIANG Yong-liang SHAO Xiao-mei ZHAO Xiao-yun HE Xiao-fen FANG Jian-qiao*   

  1. Department of Neurobiology and Acupuncture Research, the Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China
  • Received:2014-11-13 Revised:2015-01-13 Online:2015-04-06 Published:2015-04-06
  • Contact: FANG Jian-qiao E-mail:fangjianqiao7532@163.com
  • Supported by:

    National Natural Science Fund

摘要:

目的 探讨痛抑郁二联征模型大鼠中缝背核(DRN)5羟色胺(5-HT)阳性细胞的分布特点。 方法 20只雄性SD大鼠随机分为对照组和模型组。模型组大鼠连续3d皮下注射利血平,每日1次,以诱发痛抑郁二联征模型。模型制作后第1天和第2天检测左后足机械缩足阈观察大鼠痛阈变化,模型制作后第2天旷场实验和高架O迷宫实验检测大鼠情绪变化,免疫荧光技术观察DRN前囟后6.8mm、7.3mm和7.8mm水平的5-HT表达。 结果 模型组大鼠经利血平注射后机械痛阈显著下降,并产生抑郁样行为。对照组的DRN前囟后6.8mm、7.3mm和7.8mm水平的5-HT阳性细胞表达量分别为106.00±10.21、96.67±24.50和195.67±2.33。模型组相应的表达量分别为61.67±14.53,72.33±34.35和53.67±26.77。除前囟后7.8mm水平模型组与对照组相比有显著性差异(P<0.05)之外,其他两个水平5-HT阳性细胞表达量模型组和对照组之间差异无显著性(P>0.05)。 结论 利血平诱导的痛抑郁二联征大鼠模型以及中缝背核5-HT表达的降低,可能主要与DRN前囟后7.8mm水平的5-HT阳性细胞数变化有关,而与前囟后6.8mm和后7.3mm 水平无关。

关键词: 痛抑郁二联征, 5羟色胺, 中缝背核, 利血平, 免疫荧光, 旷场实验, 高架0迷宫实验, 大鼠

Abstract:

Objective To observe the distribution of 5-hydroxytryptamine (5-HT) in the dorsal raphe nucleus (DRN) of the rat with pain-depression dyad. Methods Twenty male SD rats were randomly divided into normal group and model group (n=10). The model group had pain-depression dyad induced by subcutaneously injecting reserpine daily for three consecutive days. The behavior was tested by mechanical pain thresholds and the depressive emotion was examined by open-field test and elevated-zero-maze test. The expressions of 5-HT in DRN (bregma-6.8,-7.3,-7.8mm) were detected with immunofluorescence test. Results Compared with the normal group, rats in the model group showed a declined pain threshold of the left hind leg and depressive-like behavior. The 5-HT labeled cells at different levels of DRN (bregma-6.8,-7.3,-7.8mm) of normal rats were 106.00±10.21, 96.67±24.50 and 195.67±2.33, while the model group rats were 61.67±14.53, 72.33±34.35 and 53.67±26.77. In comparison with the normal group, the expressions of 5-HT in DRN (bregma-7.8mm) decreased significantly in the model group (P<0.05), but no changes were observed at the levels of bregma-6.8 and-7.3mm (P>0.05). Conclusions The pain-depression dyad induced by reserpine injection can cause the declination of 5-HT expressions in DRN. The declination may be related with the expressions of 5-HT in DRN (bregma-7.8mm), but not with the levels of bregma-6.8 and-7.3mm.

Key words: Pain-depressiondyad, 5-Hydroxytryptamine, Dorsal raphe nucleus, Reserpine, Imm-unofluorescence, Open fied test, Elevated zero maze test, Rat