膜联蛋白A7在胃癌组织中的表达和意义

doi:10.16098/j.issn.0529-1356.2015.04.014

解剖学报 ›› 2015, Vol. 46 ›› Issue (4): 528-531.doi: 10.16098/j.issn.0529-1356.2015.04.014

膜联蛋白A7在胃癌组织中的表达和意义

张扬^1,2许倩¹ 陈龙¹ 王小杰¹ 田焕娜¹ 李春辉³李欣^1*

1.承德医学院基础医学研究所,河北承德 067000; 2.湖南省衡阳市中心医院病理科，湖南衡阳 421001; 3.承德医学院附属医院病理科，河北承德 067000

收稿日期:2014-10-20 修回日期:2015-01-19 出版日期:2015-08-06 发布日期:2015-08-06
通讯作者: 李欣 E-mail:cd-lixin@163.com

Expression of annexin A7 in human gastric carcinomas and the clinic significance

ZHANG Yang ^1,2 XU Qian¹CHEN Long¹ WANG Xiao-jie¹ TIAN Huan-na¹ LI Chun-hui³ LI Xin^1*

1. Institute of Basic Medical Sciences, Chengde Medical College, Hebei Chengde 067000, China; 2. Department of Pathology, Central Hospital in Hengyang Hunan, Hu’nan Hengyang 421001, China; 3. Department of Pathology, Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China

Received:2014-10-20 Revised:2015-01-19 Online:2015-08-06 Published:2015-08-06
Contact: LI Xin E-mail:cd-lixin@163.com

摘要/Abstract

摘要：

目的检测膜联蛋白A7（ANXA7）在胃癌组织中的表达改变，探讨胃癌的发生机制。方法收集人胃癌组织55例及正常胃组织25例，细胞裂解液裂解组织细胞，蛋白定量试剂盒进行蛋白定量后，Western blotting法和Real-time PCR法分别于蛋白水平和基因转录水平检测ANXA7在胃癌组织和正常胃组织中的表达改变，并用免疫组织化学法对ANXA7在细胞中的定位和表达进行检测。结果 ANXA7在胃癌中蛋白水平和转录水平的表达均显著增高，与正常组相比差异有显著性（P<0.05）； ANXA7在胃癌细胞中定位于细胞质。结论膜联蛋白A7在胃癌中的表达增高有可能是胃癌发生的机制之一。

关键词: 膜联蛋白A7, 胃癌, 免疫印迹法, 实时定量聚合酶链反应, 人

Abstract:

Objective To dectect the expression of annexin A7 in human gastric carcinoma so as to detect the mechanism. Methods Fifty five gastric carcinoma samples and 25 normal human gastric samples were collected. Each sample was lysed in lysis buffer. Whole protein of the supernatant was estimated by BCA-100 Protein Quantitative Analysis Kit. The expression of annexin A7 in human gastric carcinoma and normal gastric tissue was examined with Western blotting and Real\|time PCR. Immunehistolchemistry was used to detect the expression position of annexin A7 in the gastric carcinoma cells. Results The expression of annexin A7 increased in gastric cancer not only on protein level but also on RNA level when compared with normal gastric tissues, the difference was significant (P<0.05); Annexin A7 mainly expressed in the cytoplasm of the gastric carcinoma cells. Conclusion Expression of annexin A7 is up-regulated in human gastric cancer, and it may involve in the development of gastric cancer.

Key words: Annexin A7, Gastric cancer, Western blotting, Real-time PCR, Human

张扬许倩陈龙王小杰田焕娜李春辉李欣*. 膜联蛋白A7在胃癌组织中的表达和意义[J]. 解剖学报, 2015, 46(4): 528-531.

HANG Yang XU Qian CHEN Long WANG Xiao-jie TIAN Huan-na LI Chun-hui LI Xin*. Expression of annexin A7 in human gastric carcinomas and the clinic significance[J]. AAS, 2015, 46(4): 528-531.

参考文献

［1］Rand JH. The annexinopathies: a new category of diseases［J］. Arch Oncol, 2006, 14 (1-2):23-25.
［2］Srivastava M, Bubendorf L, Srikantan V, et,al. ANX7, a candidate tumor suppressor gene for prostate cancer［J］. Proc Natl Acad Sci USA, 2001,98(8):4575-4580. 
［3］Srivastava M1, Torosyan Y, Raffeld M. ANXA7 expression represents hormone-relevant tumor suppression in different cancers［J］. Int J Cancer, 2007,121(12):2628-2636.
［4］Kataoka TR, Ito A, Asada H,et,al. Annexin VII as a novel marker for invasive phenotype of malignant melanoma［J］. Jpn J Cancer Res, 2000, 91(1):75-83.
［5］Srivastava M, Montagna C, Leighton X,et al. Haploinsufficiency of Anx7 tumor suppressor gene and consequent genomic instabilitypromotes tumorigenesis in the Anx7(+/-) mouse［J］.Proc Natl Acad Sci, 2003,100(24):14287-14292. 
［6］Hung KS, Howng SL. Prognostic significance of annexin VII expression in glioblastomas multiforme in humans［J］. J Neurosurg， 2003,99(5):886-892.
［7］Leighton X, Srikantan V, Pollard HB. Significant allelic loss of ANX7region (10q21) in hormone receptor negative breast carcinomas［J］. Cancer Lett， 2004,210(2):239-244.
［8］Srivastava M, Bubendorf L, Raffeld M, et al. Prognostic impact of ANX7-GTPase in metastatic and HER2-negative breast cancer patients［J］. Clin Cancer Res， 2004, 10(7):2344-2350.［9］Qazi AS, Sun M, Huang Y, et al. Subcellular proteomics: determination of specific location and expression levels of lymphatic metastasis associated proteins in hepatocellular carcinoma by subcellular fractionation［J］. Biomed Pharmacother， 2011, 65(6):407-416. 
［10］Hsu PI, Huang MS, Chen HC,et al. The significance of ANXA7 expression and its correlation with poor cellular differentiation and enhanced metastatic potential of gastric cancer［J］. J Surg Oncol， 2008, 97(7):609-614. 
［11］Sun MZ, Liu S, Tang J, et al. Proteomics analysis of two mice hepatocarcinoma ascites syngeneic cell lines with high and low lymph node metastasis rates provide potential protein markers for tumor malignancy attributes to lymphatic metastasis［J］. Proteomics， 2009, 9(12):3285-3302.
［12］Wang XJ, Li X. Influence of annexin A7 low expression on proliferation of HepG2 cells［J］. Acta Anatomica Sinica, 2013,44(5): 656-660.(in Chinese)
王小杰，李欣.膜联蛋白A7低表达对人肝癌HepG2细胞增殖的影响［J］. 解剖学报，2013，44（5）：656-660.
［13］Tian HN, Wang XJ, Chen L, et al. Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells［J］. Acta Anatomica Sinica,2015,46(2): 216-220.(in Chinese)
田焕娜，王小杰，陈龙，等. 膜联蛋白A7低表达对胃癌MGC-803细胞生物学特性的影响［J］.解剖学报，2015,46(2): 216-220.

[1]	洪晓敏李三强崔钦奕郑润月杨孟利罗仁利李前辉 . 酒精性肝纤维化核因子κB信号通路与性别的差异 [J]. 解剖学报, 2024, 55(1): 55-61.
[2]	谢惠兰方芳林毅. Chir99021调控Wnt/β-catenin 信号通路抑制大鼠牙髓干细胞成骨诱导分化的机制[J]. 解剖学报, 2024, 55(1): 67-72.
[3]	魏茜茜李超红赵晨露唐家萍刘玉珍. 大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响 [J]. 解剖学报, 2024, 55(1): 3-9.
[4]	刘晓丽许琳吴爱雪闻彩云李如画吴安婷陈黛茜陈成春. 多发性硬化和视神经脊髓炎的脑灰质结构分析 [J]. 解剖学报, 2024, 55(1): 17-24.
[5]	袁蕾杨智伟杨惠刘政海何洁万炜. 三七总皂苷抑制小鼠星形胶质细胞活化缓解完全弗氏佐剂所致炎性痛 [J]. 解剖学报, 2024, 55(1): 25-31.
[6]	马婷婷陈建红刘爱翠李海宁. 抑制lncRNA TUG1下调核苷酸结合寡聚结构域样受体蛋白1炎症小体在延缓阿尔茨海默病进展的作用 [J]. 解剖学报, 2024, 55(1): 32-42.
[7]	邹成功冯浩陈兵唐辉邵川孙谋杨荣何家全. 创伤性颅脑损伤中神经功能障碍与认知功能损伤的动态变化 [J]. 解剖学报, 2024, 55(1): 43-48.
[8]	吕海燕沈西雅赵富生朱梅. 松茸多糖对 1-甲基-4-苯基-吡啶离子诱导 PC12 细胞损伤的影响 [J]. 解剖学报, 2024, 55(1): 49-54.
[9]	白贺滕野冯蕾孟海伟汤煜春刘树伟. 基于空间域与频域特征自适应融合和类间边界区域增强的三维海马分割[J]. 解剖学报, 2024, 55(1): 73-81.
[10]	郑丽平刘霞杜晓华吴亚娟刘珊珊 . 脑源性神经营养因子在牦牛与黄牛端脑不同区域的表达与分布 [J]. 解剖学报, 2024, 55(1): 10-16.
[11]	尹诗琴杨思艺王锐涵游贵宣杨迎秋张磊. 基于CT平扫下胫腓联合腓骨切迹分型及其临床意义[J]. 解剖学报, 2024, 55(1): 82-87.
[12]	杨洋史君李琨马渊张少杰侯二飞王超群陈杰王星李志军 . 切除不同范围钩突后颈椎椎间盘的有限元分析[J]. 解剖学报, 2024, 55(1): 88-97.
[13]	孙雷王星宇谢水华. 老年骨质疏松性胸腰椎压缩性骨折患者PKP术后再发骨折的风险分析及列线图预测模型的构建[J]. 解剖学报, 2024, 55(1): 98-104.
[14]	郝永明郭磊周程辉裴汉军李莉赵哲 . 改良腹主动脉缩窄法建立心肌肥厚模型[J]. 解剖学报, 2024, 55(1): 120-124.
[15]	许亚平余悦欣陈金福王柯柯郭志坤 . 高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制 [J]. 解剖学报, 2023, 54(6): 716-721.

膜联蛋白A7在胃癌组织中的表达和意义

Expression of annexin A7 in human gastric carcinomas and the clinic significance

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价