RNAi靶向沉默对人卵巢癌OVCAR3细胞CD105和Ki67基因表达的影响

doi:10.16098/j.issn.0529-1356.2016.04.011

解剖学报 ›› 2016, Vol. 47 ›› Issue (4): 502-506.doi: 10.16098/j.issn.0529-1356.2016.04.011

RNAi靶向沉默对人卵巢癌OVCAR3细胞CD105和Ki67基因表达的影响

郭海荣¹ 王晓燕¹ 贺帅² 刘萍^1*

1. 内蒙古医科大学第三附属医院妇产科，内蒙古自治区包头 014010; 2. 包头医学院基础医学与法医学院病理学教研室，内蒙古自治区包头 014010

收稿日期:2015-04-21 修回日期:2016-03-28 出版日期:2016-08-06 发布日期:2016-08-06
通讯作者: 刘萍 E-mail:liupingbg@126.com
基金资助:
CD105与Ki67联合表达在卵巢上皮癌中的临床意义及机制研究

Impacts of RNAi silencing on the CD105 and Ki67 gene expression in ovarian cancer OVCAR3 cell lines

GUO Hai-rong¹WANG Xiao-yan¹ HE Shuai²LIU Ping ^1*

1. Department of Obstetrics and Gynecology，the Third Affiliated Hospital of Innermongolia Medical University，Neimenggu Baotou 014010, China; 2. Department of Pathology, Shcool of Basic Medical and Forensic Science, Baotou Medical College，Neimenggu Baotou 014010, China

Received:2015-04-21 Revised:2016-03-28 Online:2016-08-06 Published:2016-08-06
Contact: LIU Ping E-mail:liupingbg@126.com

摘要/Abstract

摘要：

目的探讨RNAi靶向沉默对人卵巢癌OVCAR3细胞CD105和Ki67基因的表达。方法采用脂质体介导的基因法将不同浓度的CD105-siRNA、 Ki67-siRNA转染至体外培养的人卵巢癌OVCAR3细胞中，检测干扰前后人卵巢癌细胞中CD105、Ki67基因表达的变化以确定沉默效果。构建CD105-siRNA、Ki67-siRNA及各自的阴性对照序列并高效地转染人卵巢癌OVCAR3细胞，采用Western blotting和Real-time PCR从蛋白和基因水平检测CD105和Ki67的表达变化。结果转染CD105-siRNA或Ki67-siRNA终浓度为50nmol/L和100nmol/L的人卵巢癌细胞中CD105mRNA或Ki67mRNA的相对表达水平及CD105和Ki67的表达均明显低于空白对照组和阴性对照组（NC1），差异具有统计学意义（P＜0.001）。结论 CD105-siRNA和Ki67-siRNA能特异性抑制人卵巢癌OVCAR3细胞中的CD105和Ki67基因表达，下调mRNA和蛋白的表达水平。

关键词: CD105, Ki67, 实时定量聚合酶链反应, 免疫印迹法, 人

Abstract:

Objective To detect changes of the CD105 and Ki67 gene expression and to determine the effect of gene silence on OVCARs cells transfected these gene sequences by liposome lipofectamine TM2000 respectively. Methods OVCAR3 were transfected with siRNA of CD105-siRNA group, Ki67-siRNA group and negative control groups in vitro respectively. The expressions of CD105mRNA, Ki67mRNA and their proteins were detected with Real-time PCR and Western blotting. Results The intensities of relative expressions for both CD105 mRNA and Ki 67mRNA were significantly lower in both siRNA-CD105 transfected and siRNA-Ki67-transfected OVCARs than those in control groups(P<0.001). Conclusion CD105-siRNA and Ki67-siRNA can specifically inhibit CD105, Ki67 gene expression levels in ovarian cancer OVCAR3 cells and lowered their mRNA and protein expression.

Key words: CD105, Ki67, Real-time PCR, Western blotting, Human

郭海荣王晓燕贺帅刘萍. RNAi靶向沉默对人卵巢癌OVCAR3细胞CD105和Ki67基因表达的影响[J]. 解剖学报, 2016, 47(4): 502-506.

GUO Hai-rong WANG Xiao-yan HE Shuai LIU Ping. Impacts of RNAi silencing on the CD105 and Ki67 gene expression in ovarian cancer OVCAR3 cell lines[J]. Acta Anatomica Sinica, 2016, 47(4): 502-506.

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RNAi靶向沉默对人卵巢癌OVCAR3细胞CD105和Ki67基因表达的影响

Impacts of RNAi silencing on the CD105 and Ki67 gene expression in ovarian cancer OVCAR3 cell lines

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