多种脊髓损伤致继发性肠道功能障碍的模型比较

doi:10.16098/j.issn.0529-1356.2017.06.003

摘要/Abstract

摘要：

目的通过3种不同方法制备大鼠脊髓损伤致继发性肠道功能障碍的模型，探究各组脊髓损伤致继发性肠道功能障碍模型的表现差别，为脊髓损伤继发肠道功能障碍的相关研究提供稳定的实验动物模型复制方法和实验基础。方法将48只SD大鼠随机分为打击组（A组，n=12）、全横断组（B组，n=12）、钳夹组（C组，n=12）、假手术组（D组，n=12）。于术后1 d、3d、7 d分别对各组进行BBB功能评分；肠肌电慢波测定；血清D-乳酸含量测定；HE染色观察肠组织形态。结果术后1 d、3d、7 d，A、B、C组BBB评分、肌电慢波振幅均低于D组（P<0.05）;Chiu肠黏膜评分、血清D-乳酸浓度均显著高于D组（P<0.05）。术后第7天， A组血清D-乳酸浓度和Chiu肠黏膜评分与B、C两组间差异存在显著性（P<0.05）。结论不同方法制备脊髓损伤后，各组均继发肠道功能障碍，其中A组模型复制较为稳定，且死亡率较低，更适用于周期较长的实验研究。

关键词: 脊髓损伤, 肠道功能障碍, 比较研究, 肠肌电慢波, D-乳酸, HE染色, 大鼠

Abstract:

Objective The model of intestinal dysfunction after spinal cord injury in rats was prepared with three different methods. The objective of this study was to explore the difference of the groups’ performance, and to provide a stable experimental animal model replica technique and reliable experiment basis for the research of secondary intestinal dysfunction caused by spinal cord injury. Methods Forty-eight Sprague Dawley rats were randomly divided into four groups, 12 rats per group: attack group (A), total traverse group (B), clamps group (C), and sham operation group (D). The BBB function test, myoelectric slow wave activity measurement, serum D-lactic acid content determination, and HE staining to observe intestinal tissue morphology were performed in 1 day, 3days and 7 days after modeling. Results The BBB functional scores and the amplitude of the myoelectricity slow wave of A, B and C groups in 1day, 3days and 7 days were lower than that of group D (P<0.05). The Chiu intestinal mucosa scores and serum D-lactic acid concentration were significantly higher than that of group D (P<0.05). After 7 days of modeling, there were significant differences between group A and groups B and C in serum D-lactic acid concentration (P<0.05). Conclusion Using different method to prepare the model of the spinal cord injury, groups A, B and C all showed the secondary intestinal dysfunction. However, the model replication of group A was more stable, and the mortality was lower, which is suitable for longer experimental cycle.

Key words: Spinal cord injury, Gastrointestinal dysfunction, Comparative research, Myoelectric slow wave activity;D-lactate, HE staining, Rat

杨悦璠汪益李亭肖杰欣王茜杨森陈勇易巧孔欣欣杨平张晓杨拯. 多种脊髓损伤致继发性肠道功能障碍的模型比较[J]. 解剖学报, 2017, 48(6): 651-657.

YANG Yue-fan WANG Yi LI Ting XIAO Jie-xin WANG Xi YANG Sen CHEN Yong YI Qiao KONG Xin-xin YANG Ping ZHANG Xiao YANG Zheng. Comparison of intestinal dysfunction models after multiple spinal cord injuries in rats[J]. Acta Anatomica Sinica, 2017, 48(6): 651-657.

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