解剖学报

解剖学报 ›› 2018, Vol. 49 ›› Issue (1): 63-69.doi: 10.16098/j.issn.0529-1356.2018.01.010

• 肿瘤生物学 • 上一篇    下一篇

黄芪多糖联合顺铂对小鼠Lewis肺癌移植瘤Caspase-3、Smac/Diablo表达的影响

 庄梦婕1,4 刘丹1,4陈彦文2,4明海霞3,4*   

  1. 1. 甘肃中医药大学中西医结合基础学科; 2. 甘肃中医药大学基础医学院; 3. 甘肃中医药大学临床医学院; 4. 甘肃省中药药理与毒理学重点实验室，甘肃省高校重大疾病分子医学与中医药防治研究重点实验室， 兰州 730000
  • 收稿日期:2017-03-29 修回日期:2017-09-07 出版日期:2017-02-06 发布日期:2018-02-06
  • 通讯作者: 明海霞 E-mail:18461937905@163.com
  • 基金资助:
    甘肃省高校基本科研业务项目;兰州市科技局计划项目;研究生创新基金

Astragalus polysaccharide inhibiting Lewis lung carcinoma transplanted tumor in mice and influences the expression of Caspase-3 and Smac/Diablo

ZHUANG Meng-jie 1,4 LIU Dan 1,4 CHEN Yan-wen 2,4 MING Hai-xia 3,4*   

  1. 1. Basic Discipline of Chinese and Western Integrative, Gansu University of Chinese Medicine; 2.Basic Medical school, Gansu University of Chinese Medicine;3.Clinical Medicine College, Gansu University of Chinese Medicine; 4. Provincial Laboratory of Chinese Medicine Pharmacology and Toxicology;Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research,in Gansu Colleges and Universities, Lanzhou 730000 China
  • Received:2017-03-29 Revised:2017-09-07 Online:2017-02-06 Published:2018-02-06
  • Contact: MING Hai-xia E-mail:18461937905@163.com

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摘要：

目的 观察黄芪多糖（APS）及联合顺铂（DDP）对小鼠Lewis肺癌（LLC）移植瘤凋亡蛋白Caspase-3、Smac/Diablo表达的影响，探讨黄芪多糖抗肿瘤的机制。方法 90只C57BL/6 J小鼠，随机分为9组，正常组、模型组、APS低、中、高剂量组、DDP组、联合低、中、高剂量组，每组10只。除正常组外，其余80只均接种肺癌移植瘤细胞（1×1010/L）于右前肢腋窝皮下，制造模型为荷瘤小鼠。制造模型次日起，治疗组的小鼠给予腹腔注射0.3 ml药物。顺铂每周注射1次，其余药物每日1次，正常组和模型组注射等体积生理盐水，连续20 d，于第21天处死。肿瘤组织进行HE染色并行病理学观察；免疫组织化学染色和图像分析方法检测移植瘤细胞中的Caspase-3及Smac/Diablo的表达。 结果 荷瘤小鼠在APS（高）、联合（中）、联合（高）组的体质量变化，具有统计学意义。与模型组小鼠比较，肿瘤组织的病理组织学HE显示，APS（高）联合顺铂组的肿瘤细胞坏死最为明显；免疫组织化学法表明，治疗组的肿瘤组织中Caspase-3、Smac/Diablo蛋白表达水平均升高，联合（高）组升高最明显。 结论 APS及联合化疗药物DDP能抑制小鼠Lewis肺癌细胞的生长，其机制可能与升高Caspase-3、Smac/Diablo的表达有关。

关键词: 黄芪多糖, 顺铂, Lewis肺癌, 免疫组织化学, 免疫印迹法, 小鼠

Abstract:

Objective To observe the effect of Astragalus polysaccharides (APS) combined with cisplatin (DDP) on the expressions of Caspase-3 and Smac/Diablo in the mice with transplantated tumors Lewis lung carcinoma (LLC). Methods Ninety C57BL/6 J mice were randomly divided into normal control group, model group, and 50, 100 or 200 mg/L APS group, 6 mg/kg cisplatin group, and 3 mg/kg cisplatin combined with 50, 100 or 200 mg/L APS group; 10 mice per group. Except the mice in normal group, the rest mice were inoculated with LLC cells (1×1010/L) in the right fore axillary subcutaneous tissue to establish a model of tumorbearing mice. In the second day of building the animal model, the mice in the treatment group were given intraperitoneal injection of 0.3 ml of the drug. At the same time, the mice in the cisplatin group were given once a week, and the rest of the group mice once a day. The mice in the normal and model groups were given the same amount of saline injection for 20 days. All mice were killed on the 21st day. Tumor tissue lesions were observed by HE staining. The expression and location of Caspase-3 and Smac/Diablo proteins in transplanted tumor tissues were detected by immunohistochemical staining and image analys is method . Results The weights of mice were decreased in the 100 and 200 mg/L APS group and 3 mg/kg cisplatin combined with 50, 100, 200 mg/L APS group. Compared with the model group, the necrosis of tumor tissues in the 200 mg/L APS combined with 3 mg/kg cisplatin group was most obvious. The expression of Caspase-3 and Smac/Diablo was increased in the treatment group. The increasing of tumor tissues in 200 mg/L APS combined with 3 mg/kg cisplatin group was most obvious. Conclusion APS and APS combined with cisplatin restrain the growth of Lewis lung cancer in C57BL/6 J mouse, which may depend on increase of the expression of Caspase-3 and Smac/Diablo.

Key words: Astragalus polysaccharides, Cisplatin, Lewis lung carcinoma, Immunohistochemistry, Western blotting, Mouse