解剖学报 ›› 2015, Vol. 46 ›› Issue (5): 690-693.doi: 10.16098/j.issn.0529-1356.2015.05.020

• 技术方法 • 上一篇    下一篇

甲基丙烯酸羟乙酯塑料包埋切片技术在小鼠胎脑组织学分析中的应用

李帅 陈宇琛 张雪茜 童仙杰 宋晓彬 范春娥 陈华群*   

  1. 南京师范大学生命科学学院 江苏省分子医学生物技术重点实验室,南京 210023
  • 收稿日期:2015-02-03 修回日期:2015-04-22 出版日期:2015-10-06 发布日期:2015-10-06
  • 通讯作者: 陈华群 E-mail:chenhuaqun@njnu.edu.cn
  • 基金资助:

    ZIP激酶对于小鼠血管内皮细胞通透性和血管形成的调节;内皮细胞ZIP激酶调节血管发育及血管生成的研究

Application of hydroxyethyl methacrylat plastic-embedded sectioning technology in the histological analysis of the brain tissue of mouse embryo

LI Shuai CHEN Yu-chen ZHANG Xue-xi TONG Xian-jie SONG Xiao-bing FAN Chun-e CHEN Hua-qun*   

  1. Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Jiangsu, Nan Jing 210023, China
  • Received:2015-02-03 Revised:2015-04-22 Online:2015-10-06 Published:2015-10-06
  • Contact: CHEN Hua-qun E-mail:chenhuaqun@njnu.edu.cn

摘要:

目的 探索塑料包埋切片法在小鼠胎脑组织学分析中的应用。 方法 取胚胎期13.5d(E13.5)小鼠,4% 多聚甲醛4℃固定过夜,分离胎鼠头部,甲基丙烯酸羟乙酯(HEMA)塑料包埋组织并切片;对切片进行HE 染色。 结果 与石蜡包埋相比,采用HEMA塑料包埋的方法进行胎鼠脑组织学分析,形态结构保存较完整、HE染色效果较清晰。 结论 利用塑料包埋技术进行小鼠胎脑组织学分析,优于石蜡包埋方法。

关键词: 塑料包埋, 甲基丙烯酸羟乙酯, 胚胎, 脑组织, HE染色, 小鼠

Abstract:

Objective To study the application of plastic embedding technology in the histological analysis of the brain of mouse embryo. Methods The E13.5 embryos were fixed in 4% polyformaldehyde 4℃ overnight. The head was dissected and embedded in hydroxyethyl methacrylat (HEMA). The blocks were sectioned at 4μm thick and stained with HE staining. Results Compared with paraffin embedded sections, the HEMA embedded brains showed much better morphologic and structure preservation, as well as higher quality of HE staining images. Conclusion The HEMA plastic embedding is better than paraffin embedding in the histological analysis of the brain tissue of the mouse embryo.

Key words: Plastic embedding, HEMA, Mouse embryo, HE staining