三类生物因子对涡虫生殖和再生的影响

doi:10.16098/j.issn.0529-1356.2017.06.017

摘要/Abstract

摘要：

目的以国内广布种日本三角涡虫（Dugesia japonica）为受试动物，探讨三类生物因子对涡虫生殖和再生的影响。方法选择与生殖相关的激素（雌二醇、甲基睾丸素），生物胺（5-羟色胺、多巴胺）和D-氨基酸（D-色氨酸、D-精氨酸、D-苯丙氨酸）对日本三角涡虫进行急性毒性实验，用SPSS 17. 0统计学软件计算出适宜的实验浓度，对整体及再生涡虫进行培养，观察并记录实验现象。结果雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸处理组分别引起20%、40%、20%和20%涡虫卵巢及交配器官的发育；而多巴胺则引起40%～50%涡虫生殖腺退化。另外，5-羟色胺处理组涡虫头部再生速度加快，而多巴胺处理组则头部再生速度减慢。结论雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸能够诱导涡虫生殖腺的发育，并且5-羟色胺促进涡虫头部再生；多巴胺则引起涡虫生殖腺退化并对涡虫再生有抑制作用。

关键词: 性化, 再生, 激素, 生物胺, D-氨基酸, 急性毒性实验, 日本三角涡虫

Abstract:

Objective To investigate the effects of the three reproductive-relative factors on gonads and regeneration in planarians. Methods In this study, the planarians Dugesia japonica were cultured with three reproductive-relative factors: the hormones ［estradiol (E2), methyltestosteronum(MT)］, biogenic amine ［5-hydroxy-tryptamine (5-HT),dopamine (DA)］ and D-amino acids (D-tryptophan, D-arginine, D-phenylalanine). The cultured concentration was calculated by SPSS17.0 after the acute toxicity experiment, and the experimental phenomena were recorded. Results E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, which proportions were 20%, 40%, 20%, and 20% respectively, but DA made the gonads atrophy in 40% or 50% planarians. Besides, 5-HT increased speed of regeneration, while DA inhibited it during planarians regeneration. Conclusion E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, and 5-HT increased speed of regeneration; DA inhibited the gonads and regeneration.

Key words: Sexualization, Regeneration, Hormone, Biogenic amine, D-amino acid, Acute toxicity experiment, Dugesia japonica

董自梅董艳萍陈广文刘德增. 三类生物因子对涡虫生殖和再生的影响[J]. 解剖学报, 2017, 48(6): 732-739.

DONG Zi-mei DONG Yan-ping CHEN Guang-wen LIU De-zeng. Influence of three kinds of biological factors on the reproduction and regeneration in planarians[J]. Acta Anatomica Sinica, 2017, 48(6): 732-739.

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