解剖学报

解剖学报 ›› 2020, Vol. 51 ›› Issue (3): 456-460.doi: 10.16098/j.issn.0529-1356.2020.03.025

• 技术方法 • 上一篇    下一篇

一种颈总动脉部分结扎-内膜新生模型的制备

姜志龙 姜悦 孙文清 陈小康 张又枝*   

  1. 湖北科技学院药学院，湖北 咸宁 437100
  • 收稿日期:2019-08-22 修回日期:2019-11-21 出版日期:2020-06-06 发布日期:2020-06-06
  • 通讯作者: 张又枝 E-mail:yzzhang242@hbust.edu.cn
  • 基金资助:
    国家自然科学基金青年基金

A model of partial ligation of common carotid artery and neointima

JIANG Zhi-long JIANG Yue SUN Wen-qing CHEN Xiao-kang ZHANG You-zhi*    

  1. School of Pharmacy, Hubei University of Science and Technology，Hubei Xianning 437100, China
  • Received:2019-08-22 Revised:2019-11-21 Online:2020-06-06 Published:2020-06-06
  • Contact: ZHANG You-zhi E-mail:yzzhang242@hbust.edu.cn

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摘要：

目的  探讨一种左颈总动脉部分结扎后诱导血管重构（内膜新生）的方法。  方法  8周龄雄性C57BL/6 J小鼠40只，随机分为6组：正常组（WT）、假手术组（sham）、术后4周、8周、10周和12周组。手术结扎左颈总动脉（LCA）远心端4个分支中的颈内、颈外和枕动脉，保留甲状腺上动脉，造成左颈总动脉血管内膜新生和血管重构。术后观察小鼠体重和饲料的消耗情况；HE染色观察血管形态学变化；天狼猩红染色观察血管胶原纤维聚集情况。  结果  除正常组小鼠外，其他组小鼠术后1～2 d体重和饮食量均降低，第3天开始上升，假手术组小鼠第2天开始上升；HE染色表明，假手术组和正常组小鼠LCA均未形成新生内膜，部分结扎手术后4、 8、10和12周组小鼠LCA均出现内膜和中膜层的增厚、管腔狭窄等。右侧未结扎的颈总动脉无内膜增厚，但出现了管腔增大；天狼猩红染色显示，内膜和中膜增生部分有胶原积聚。  结论  左颈总动脉部分结扎可以建立小鼠动脉内皮损伤模型并伴有明显的内膜新生。

关键词:  颈动脉部分结扎, 内膜新生, 纤维化, 血管重构, 天狼猩红染色, 小鼠

Abstract:

Objective  To discuss a method  to induce vascular remodeling (intimal regeneration) after partial ligation of the left common carotid artery.   Methods  Forty 8-week-old male C57BL/6 J mice were randomly divided into 6 groups: normal group (WT), sham group (sham), 4 weeks, 8 weeks, 10 weeks and 12 weeks postoperative groups. Only the internal carotid, external carotid and occipital arteries in the four distal branches of the left common carotid artery (LCA) were ligated, and the superior thyroid artery was retained, resultsing in intimal neovascularization and vascular remodeling of the left common carotid artery. The changes of body weight and forage were observed after operation. Morphological changes of blood vessels were observed by HE staining. The aggregation of collagen fibers in blood vessels was observed by sirius red staining.   Results  In addition to the WT group, the weight and dietary quantity of other groups were reduced 1-2 days after the operation, and began to rise from the third day, while the sham group began to rise from the second day. HE staining showed that no new intima was formed in the left common carotid artery of sham group and WT group, and thickening of intima and media layer and stenosis of the nasal cavity were observed in the 4, 8, 10 and 12 weeks groups after partial ligation. There was no intima thickening in the unligated carotid artery on the right side, but there was an enlargement of the lumen. This sirius red stain demonstrated collagen accumulation in the intima and tunica media. 
 Conclution  Partial ligation of the left common carotid artery can establish a mouse model of arterial endothelial injury with obvious intima regeneration.

Key words: Partial carotid ligation, Neointima, Fibrosis, Vascular remodeling, Sirius red staining, Mouse

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