多巴胺受体在小肠的分布与定位

doi:10.16098/j.issn.0529-1356.2024.05.013

解剖学报 ›› 2024, Vol. 55 ›› Issue (5): 612-618.doi: 10.16098/j.issn.0529-1356.2024.05.013

• 组织学胚胎学发育生物学 • 上一篇下一篇

多巴胺受体在小肠的分布与定位

陈钧钧¹ 周立¹ 苏田¹ 王现伟² 张海龙³ 王志勇^1*

1.新乡医学院人体解剖学与组织学胚胎学系，河南新乡 453003;2.河南省医用组织再生重点实验室，河南新乡 453003;3.河南大学基础医学院，河南开封 475000

收稿日期:2023-09-08 修回日期:2024-01-08 出版日期:2024-10-06 发布日期:2024-10-06
通讯作者: 王志勇 E-mail:wanliheng@vip.163.com
基金资助:
河南省科技公关项目;新乡医学院博士科研启动基金;河南省高等学校青年骨干教师培养计划;新乡医学院基础医学院培育项目

Distribution and localization of dopamine receptor in small intestines

CHEN Jun-jun¹ ZHOU Li¹ SU Tian¹ WANG Xian-wei² ZHANG Hai-long³ WANG Zhi-yong^1*

1.Department of Human Anatomy and Histoembryology, Xinxiang Medical University, He’nan Xinxiang 453003, China; 2.He’nan Key Laboratory of Medical Tissue Regeneration, He’nan Xinxiang 453003, China; 3.School of Basic Medicine, He’nan University, He’nan Kaifeng 475000, China

Received:2023-09-08 Revised:2024-01-08 Online:2024-10-06 Published:2024-10-06
Contact: WANG Zhi-yong E-mail:wanliheng@vip.163.com

摘要/Abstract

摘要：

目的探讨多巴胺受体D3~D5在不同物种小肠的分布和定位。方法应用免疫组织化学和免疫印迹法，分别检测D3~D5在小鼠、大鼠和恒河猴小肠的分布及表达水平，通过免疫荧光双标技术观察小肠固有层（LP）免疫球蛋白A阳性浆细胞（IgA+PC）中D3~D5的表达。结果 D3和D5在小鼠、大鼠和恒河猴小肠的上皮层、LP、黏膜下神经丛（SMP）和肌间神经丛（MP）内广泛分布；D4在小鼠和恒河猴小肠的分布与D3和D5一致，但在大鼠小肠仅分布于上皮层和LP。小鼠和大鼠LP内的IgA+PC均表达D3和D5，不表达D4。结论 D3和D5在小鼠、大鼠和恒河猴小肠具有相似的分布和定位模式，D4则存在物种差异。多巴胺可能参与调节IgA+PC的功能。

关键词: 多巴胺受体｜小肠｜免疫球蛋白A阳性浆细胞｜免疫印迹法｜免疫荧光｜小鼠｜大鼠｜恒河猴 ,

Abstract:

Objective To explore the distribution and localization of dopamine receptor D3-D5 in the small intestine of different species. Methods The distribution and expression of D3-D5 in the small intestine of mice, rats and rhesus monkeys were detected by immunohistochemistry and Western blotting. The expression of D3-D5 in immunoglobulin A positive plasma cells (IgA+PC) located in the lamina propria (LP) were detected by immunofluorescence double labeling. Results D3 and D5 were widely distributed in the epithelium, LP, submucosal plexus (SMP) and intermuscular plexus (MP) of the small intestine in mice, rats and rhesus monkeys. The distribution of D4 in the small intestinal of mice and rhesus monkeys were consistent with the result of D3 and D5. D4 was distributed only within the epithelium and LP of rat small intestine. D3 and D5 were expressed in the IgA+PC in the LP of mice and rats, whereas D4 was not. Conclusion The distribution and localization pattern of D3 and D5 are similar in the small intestine of mice, rats and rhesus monkeys, whereas those of D4 vary between different species. Dopamine may be involved in regulating the functions of IgA+PC.

Key words:

"> Dopamine receptor｜Small intestine｜Immunoglobulin A positive plasma cell｜Western blotting｜Immunofluorescence｜Mouse｜Rat｜Rhesus monkey

中图分类号:

R322.4

陈钧钧周立苏田王现伟张海龙王志勇. 多巴胺受体在小肠的分布与定位[J]. 解剖学报, 2024, 55(5): 612-618.

CHEN Jun-jun ZHOU Li SU Tian WANG Xian-wei ZHANG Hai-long WANG Zhi-yong. Distribution and localization of dopamine receptor in small intestines[J]. Acta Anatomica Sinica, 2024, 55(5): 612-618.

参考文献

［1］ Liu CZ, Zhu JX. ［Source, metabolism and function of dopamine in digestive tract］［J］. Sheng Li Xue Bao, 2020, 72(3): 336-346.

［2］ Pacheco R, Contreras F, Zouali M. The dopaminergic system in autoimmune diseases［J］. Front Immunol, 2014, 5: 117.

［3］ Ceglia S, Berthelette A, Howley K, et al. An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells［J］. Nat Immunol, 2023, 24(3): 531-544.

［4］ Ugalde V, Contreras F, Prado C, et al. Dopaminergic signalling limits suppressive activity and gut homing of regulatory T cells upon intestinal inflammation［J］. Mucosal Immunol, 2021, 14(3): 652-666.

［5］ Peng H, Yu S, Zhang Y, et al. Intestinal dopamine receptor D2 is required for neuroprotection against 1-methyl-4-phenyl-1,2,3,6tetrahydropyridine-induced dopaminergic neurodegeneration［J］. Neurosci Bull, 2022, 38(8): 871-886.

［6］ Zhou J, Duan LP, Ye SM, et al. Localization and mRNA expression of dopamine receptor subtype D4 and D5 in human stomach and duodenum［J］. Chinese Journal of Digestion, 2003，23(7): 15-18. (in Chinese)

周俊, 段丽萍, 叶嗣懋, 等. 多巴胺受体亚型D4和D5在人胃、十二指肠的定位及表达［J］. 中华消化杂志, 2003，23(7): 15-18.

［7］ Wang W, Garcia C, Shao F, et al. Lung dopaminergic nerves facilitate the establishment of TH2 resident memory cells in early life［J］. J Allergy Clin Immunol, 2023, 152(2): 386-399.

［8］ Steigerwald H, Krause M, Gonzalez-Menendez I, et al. Peach extract induces systemic and local immune responses in an experimental food allergy model［J］. Sci Rep, 2023, 13(1): 1892.

［9］ Feng XY, Zhang DN, Wang YA, et al. Dopamine enhances duodenal epithelial permeability via the dopamine D5 receptor in rodent［J］. Acta Physiol (Oxf), 2017, 220(1): 113-123.

［10］ Osorio-Barrios F, Navarro G, Campos J, et al. The heteromeric complex formed by dopamine receptor D5 and CCR9 leads the gut homing of CD4+ T cells upon inflammation［J］. Cell Mol Gastroenterol Hepatol, 2021, 12(2): 489-506.

［11］ Liu L, Wu Y, Wang B, et al. DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization［J］. Cell Death Dis, 2021, 12(6): 500.

［12］ Li ZS, Schmauss C, Cuenca A, et al. Physiological modulation of intestinal motility by enteric dopaminergic neurons and the D2 receptor: analysis of dopamine receptor expression, location, development, and function in wild-type and knock-out mice［J］. J Neurosci, 2006, 26(10): 2798-2807.

［13］ Zhang XL, Sun Q, Quan ZS, et al. Dopamine regulates colonic glial cell-derived neurotrophic factor secretion through cholinergic dependent and independent pathways［J］. Br J Pharmacol, 2024，181（3）：423-428.

［14］ Rojas OL, Probstel AK, Porfilio EA, et al. Recirculating intestinal IgA-producing cells regulate neuroinflammation via IL-10［J］. Cell, 2019, 176(3): 610-624 e18.

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多巴胺受体在小肠的分布与定位

Distribution and localization of dopamine receptor in small intestines

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