›› 2010, Vol. 41 ›› Issue (6): 837-841.doi: 10.3969/j.issn.0529-1356.2010.06.012

• 论著 • 上一篇    下一篇

Periostin表达对鼻咽癌细胞体外侵袭行为的影响

李美香1*; 谢远杰1;石金凤2;赵国军1; 张晓红1; 刘月顺2; 龙治峰2   

  1. 1.南华大学医学院组织学胚胎学教研室; 2.显微形态实验中心,湖南 衡阳 421001
  • 收稿日期:2010-02-10 修回日期:2010-04-12 出版日期:2010-12-06
  • 通讯作者: 李美香

Effects of periostin expression on the invasiveness of nasopharyngeal carcinoma cells EM>in vitro/EM>

  1. 1.Department of Histology and Embryology University of South China, Hu’nan Hengyang 421001, China;2.Laboratory Center of Microscope and Morphology, University of South China, Hu′nan Hengyang 421001, China
  • Received:2010-02-10 Revised:2010-04-12 Online:2010-12-06
  • Contact: LI Mei-xiang

关键词: Perisotin, 鼻咽癌, 转移, 基质金属蛋白酶, 转染, 明胶酶谱法, Boyden小室

Abstract: Objective To investigate the effects and mechanism of periostin on their invasiveness of nasopharyngeal carcinoma cells EM>in vitro/EM>. Methods Western blotting was employed to detect the expression levels of periostin in the stroma of nasopharyngeal carcinoma (NPC) and normal nasopharyngeal mucosa (NNM), nasopharyngeal carcinoma cells 6-10B (low metastatic potential cell line ) and 5-8F (high metastatic potential cell line), NIH 3T3 fibroblasts. Eukaryotic expression plasmids of periostin were constructed and transfected into NIH 3T3 fibroblasts to obtain transient transfection cells, the expression of periostin was detected by Western blotting in NIH 3T3 cells before and after transfection. The biological effects were observed, including in vitro invasion by Boyden charmber assay, matrix metalloproteinase(MMPs) activation by gelatin zymogram. Results The expression levels of periostin protein varied in the two different metastatic-potential nasopharyngeal carcinoma cell lines and NIH 3T3 fibroblasts. Periostin was not expressed in 6-10B and NIH 3T3 cells, and 5-8F cells showed weak expression in comparison to the stroma of nasopharyngeal carcinoma. Boyden chamber assay indicated that the invasive capacity of 6-10B was significantly promoted by co-culture with Periostin-overexpression NIH 3T3 cells, and further study by gelatin zymogram assay indicated that MMPs in supernatant of periostin-overexpression NIH 3T3 cells co-culture with 6-10B were more active than negative controls and blank controls. Conclusion Periostin overexpression may promote the invasive capacity of nasopharyngeal carcinoma cells EM>in vitro/EM>. The action may exert through regulating the activity of MMPs. The study will provide a new idea for finding stromal targets in tumor therapy,and periostin is expected to be a new target

Key words: Periostin, Nasopharyngeal carcinoma, Metastasis, Matrix metalloproteinase, Transfection, Zymography, Boyden charmber

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