关键词: X线, 胃, 组织结构, 总抗氧化能力, 谷胱甘肽过氧化物酶, 谷胱甘肽还原酶, 比色法, 仔鼠

Abstract: Objective To explore the effects of X-ray on stomach of filial mice, we investigated changes of the histological structure, activities of total antioxidant capacity(T-AOC), glutathione peroxidase(GSH-PX) and glutathione reductase (GR) in stomach after irradiation with different dosages of X-ray in development filial mice. Methods Totally 160 filial mice(birth 6-7 days)were irradiated with different dosages(0Gy, 1Gy, 3Gy, 5Gy, 7Gy) X-ray, and then detected the activities of T-AOC, GSH-PX, GR by colorimetry from all irradiated groups of different stages at day 1, day 5, day 10 and day 20 after irradiation. In addition, the changes of the gastric lesions of filial mice were observed by optical microscope from all experimental groups. Results The intensities of T-AOC, GR activities in stomach of the neonatal mice were lower in X-ray irradiated groups than that in the control group(EM>P/EM><0.05 or EM>P/EM><0.01), with the exception for 1Gy group. The intensities of GSH-PX activities in stomach of the neonatal mice were lower in 1Gy group and higher in 3Gy group than that in the control group on the first day after irradiation (EM>P/EM><0.05). The activities of enzyme increased in 1Gy group and reduced in 3Gy group at day 520 after irradiation, in other irradiated groups the intensities of GSH-PX were lower than that in the control group invariably (EM>P/EM><0.05 orEM> P/EM><0.01). With the increase of radiation dosages, the epithelial cells of stomach mucosa and gland cell of filial mice had different degrees of change. The epithelial cells of stomach mucosa were swelling, of vacuolization and fall-off. Gastric glands were untrammeled and the stomach was hemorrhaged. Conclusion X-ray radiation affects the structure of filial mouse stomach, it might be correlated with the low activities of T-AOC, GSH-PX and GR in filial mouse

Key words: X-ray, Stomach, Histological structure, Total antioxidant capacity, Glutathione peroxidase, Glutathione reductase, Colorimetry, Filial mouse