Changes of peroxiredoxin Ⅲ, catalases and superoxide dismutases expression in the heart of rats with hepatic ischemia-reperfusion injury

doi:10.16098/j.issn.0529-1356.2015.06.018

Abstract

Abstract:

Objective To observe the oxidative stress level of myocardial tissue and the changes of peroxiredoxin Ⅲ（PrxⅢ）, catalases（CAT）andsuperoxide dismutases（SOD）expression in hepatic ischemia-reperfusion injury (HIRI)model of rats. Methods Totally 12 male Wistar rats were randomly divided into two groups. The hepatic ischemia-reperfusion injury model of rats was established based on Kohli V’s methods. Only was the hepatic pedicle separated in control group. The serum, liver and heart were taken followed by 6h reperfusion. The activities of serum alanine transaminase（ALT）and lactate dehydrogenase（LDH）LDH were detected by the rate method. The morphological changes of liver and heart were observed with HE staining. The malondialdehyde (MDA) content in myocardial tissue was determined by Thiobarbituric acid colorimetric method. The expressions of PrxⅢ, CAT and SOD mRNA in myocardial tissue were evaluated by RT-PCR and protein levels of PrxⅢ, CAT and SOD were estimated by Western blotting. Results Compared with the control group, the activity of ALT and LDH in serum, MDA content in HIRI group were significantly increased. Structures of liver tissue of HIRI model were severely impaired according to the results of HE staining, but that of the heart were not changed. The mRNA and protein level of PrxⅢ, CAT and SOD in myocardial tissue of HIRI model were increased markedly. Conclusion HIRI can not cause the change of heart structures, but the myocardial tissue suffers from peroxidative damage. The antioxidase PrxⅢ, CAT and SOD may play an important role in the antioxidation effect during the process and protect the myocardial tissue.

Key words: Ischemia-reperfusion injury, Peroxiredoxin Ⅲ, Superoxide dismutase, Catalase, Oxidative stress, Western blotting, Rat

WANG Qie WU Qiong WANG Su-ling WANG Lei*. Changes of peroxiredoxin Ⅲ, catalases and superoxide dismutases expression in the heart of rats with hepatic ischemia-reperfusion injury[J]. AAS, 2015, 46(6): 832-836.

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