Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’spermatogenic cells#br#

doi:10.16098/j.issn.0529-1356.2017.03.007

Abstract

Abstract:

Objective To investigate the effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’ spermatogenic cells. Methods Forty-five 12-month-old male Wistar rats were randomly divided into three groups: young control group(YCG), natural aging group(NAG) and Heshouwuyin group(SWYG), 15 rats per group. From 16monthold, SWYG and NAG were intragastrically given Heshouwuyin and equal amount of normal saline respectively. Gavage treatment kept for 60 days. YCG at 12-month-old and the other two groups at 18-month-old entered experiment respectively. Real-time PCR, immunofluorescence staining, and Western blotting were used to detect the expression of DR6, BAX, Caspase-3, Cyt-C and 14-3-3σ. Results The expression of 14-3-3σ, a key factor in the mitochondrial apoptotic pathway, was significantly lower and the expressions of DR6, BAX, Caspase-3, and Cytc were significantly higher in the NAG than in the YCG, but intervention with Heshouwuyin significantly reversed this phenomenon. Conclusion Heshouwuyin improves the spermatogenic function of the testes by regulating expression of key genes in the mitochondrial apoptosis pathway.

Key words: Heshouwuyin, Testis, Mitochondrial apoptosis pathway, Real-time PCR, Immunofluorescence, Western blotting, Aging, Rat

AN Fu-li WANG Yu-juan LIU Hao-kun DU Yun-fan QIN Xuan-xi NIU Si-yun GAO Fu-lu. Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’spermatogenic cells#br#[J]. Acta Anatomica Sinica, 2017, 48(3): 287-295.

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