Cyclooxygenase-2 promoting the expression of E-selectin and the cerebral protective effects of breviscapine after cerebral ischemic-reperfusion injury in rats

doi:10.16098/j.issn.0529-1356.2018.06.001

Abstract

Abstract:

Objective To investigate the mechanisms of cyclooxygenase-2 (COX-2) aggravating cerebral injury and the protective effects of breviscapine after cerebral ischemic-reperfusion injury, by observing the effect of COX-2 on the expression of E-selectin and the effect of breviscapine on the expression of COX-2 and E-selectin. Methods Forty-eight SD rats were randomly divided into sham group, saline group, SC-58125 group and breviscapine treatment group, at ischemia hour 2/reperfusion hour 24. Nerve function scores were used to observe the neurobehavioral changes of rats, 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to assess the changes of cerebral infarction volume. Western blotting and enzyme-linked immunosorbent assay were used to detect the expression of COX-2 and E-selectin. Results In SC-58125 group and breviscapine treatment group, nerve function scores, cerebral infarction volume and COX-2 and E-selectinwere significantly decreased. Conclusion COX-2 aggravates inflammatory response of ischemia area by promoting the expression of E-selectin; breviscapine may play a protective role by inhibiting the expression of COX-2 and E-selectin.

Key words: Brain, Ischemia-reperfusion injury, Cyclooxygenase-2, E-selectin, Breviscapine, Western blotting, Rat

YANG Ying-chun REN Zhan-chuan CHEN Xin-ji . Cyclooxygenase-2 promoting the expression of E-selectin and the cerebral protective effects of breviscapine after cerebral ischemic-reperfusion injury in rats[J]. Acta Anatomica Sinica, 2018, 49(6): 703-707.

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