Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia

doi:10.16098/j.issn.0529-1356.2019.05.001

Abstract

Abstract:

Objective To investigate the regulation of microtubules by Nogo-A in the dorsal root ganglia during the inflammatory pain. Methods The ipsilateral paw withdrawal latency（PWL）was measured in wild type rats（WT，n=12) and Nogo-A konck-out (Nogo-A KO) rats (n=14) after complete Freunds adjuvant (CFA) injection. Western blotting and immunofluorescent staining were used to study the expression of microtubules and phosphorylated collapsin response mediator protein 2（p-CRMP2）in the dorsal root ganglion (DRG) of both groups. Results Knock out of Nogo-A in rats had attenuated the CFA-induced inflammatory hyperalgesia. The acetylated tubulin was reduced, and the expression of p-CRMP2 was increased in the DRG of the Nogo-A KO rats. Conclusion Nogo-A is involved in the regulation of inflammatory heat hyperalgesia by promoting the microtubule polymerization via CRMP2 pathway.

Key words: Nogo-A, Microtubule, Collapsin response mediator protein-2, Dorsal root ganglion, Inflammatory pain, Western blotting, Rat

HU Qi-guo LIU Huai-cun CHEN Ling ZHAO Yan WANG Jun. Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia[J]. Acta Anatomica Sinica, 2019, 50(5): 549-553.

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