Age-related changes of elastic fibers and elastic protein in rat ventricular myocardium

doi:10.3969/j.issn.0529-1356.2013.01.021

Abstract

Abstract:

Objective To observe and measure the morphological features and age-related changes of elastic fibers in the rat heart. Methods Ten rats in each group were collected from embryonic day 18, postnatal day 10, postnatal one month and postnatal three months. The distribution of myocardial elastin fibers and expression of elastin protein of the rat heart were observed by the orcein staining, immunohistochemistry, and Western blotting. The elastin protein of the four groups were quantitated by SPSS statistical software. Results The elastic fibers were extensively distributed in the epicardium, the periphery of cardiocyte and the small arterial wall. They were increasing with the growth. The morphology of the elastic fiber appeared hairs-like and no thick bundle was detected. The elastic fibers were growing quickly from the fetal to the new born stage, and slow down from the childhood to adult stage. The number of elastic fibers in the endocardium was peaked at the childhood stage, but reduced at the adult.Conclusion Elastic fibers in the left ventricle are gradually increased from the embryonic stage to adult stage. Such change may belong to the important ingredient of cardiac physiological reconstitution.

Key words: Heart, Elastin fiber, Orcein staining, Immunohistochemistry, Western blotting, Rat

ZHAI Xin-bo,WU Jun-fang,GUO Zhi-kun*,WANG Yan-li. Age-related changes of elastic fibers and elastic protein in rat ventricular myocardium[J]. AAS, 2013, 44(1 ): 106-110.

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