AAS ›› 2014, Vol. 45 ›› Issue (3): 398-404.doi: 10.3969/j.issn.0529-1356.2014.03.019

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Effects of ephedrine on ovarian histological structure

LIU Xiao-yuan1 LI Chong-yang2 YU Shi-yuan 1,3* WANG Min1 CAO Li-fan1   

  1. 1. College of Life Science, Northwest Normal University, Lanzhou 730070, China; 2. Public Security Branch, Gansu Political Science and Law Institute, Lanzhou 730070,China; 3. Editoral Department of the University Journal, Northwest Normal Unversity, Lanzhou 730030, China
  • Received:2013-11-04 Revised:2014-01-10 Online:2014-06-06 Published:2014-06-06
  • Contact: YU Shi-yuan E-mail:syyu006@nwnu.edu.cn

Abstract:

Objective To explore effects of ephedrine on epinephrine (EPI) and estradiol( E2 )concentration in blood, histological structures and activity of superoxide dismutase (SOD) and maleic dialdehyde (MDA) content in the ovary of female mice. Methods Pregnant female mice in the experimental group were treated with ephedrine(2g/L,4g/L,6g/L), and the mice in the control group were treated with saline by intraperitoneal injection. Using ELISA method to detect concentration changes of EPI and E2 in plasma, the changes in the enzymatic activity of SOD and MDA content were analyzed by colorimetry. The ovarian weight was measured, histological structures in ovary were observed by microscopy and the number of ovarian follicles were counted. Results EPI concentration in plasma of female mice increased, and the E2 concentration decreased dose-depenently in the experimental group. The activities of SOD were lower, and MDA content significantly increased compared to the control. The ovary weight of experimental groups was lower than the control group. The numbers of primary follicle, secondary follicle and atretic follicle increased and corpus luteum decreased in the experimental groups. There were pyknotic ovarian tissues, including oocyte ateophy, degradation granulose cells, and karyopyknotic granulose lutein cells in the experimental groups. Conclusion Ephedrine has a great effect on the ovarian histological structure and ovarian function of female mice, which may be correlated with the low activities of SOD.

Key words: Ovary, Ephedrine, Estradiol, Superoxide dismutase, Maleic dialdehyde, Histological structure, ELISA, Female mouse