›› 2011, Vol. 42 ›› Issue (5): 666-669.doi: 10.3969/j.issn.0529-1356.2011.05.019
• 组织学胚胎学与发育生物学 • Previous Articles Next Articles
Received:
Revised:
Online:
Contact:
Abstract: P>Objective To determine the expression and changes of histone deacetylase 1(HDAC1) during human bronchial regeneration after injury and to explore the molecular mechanisms in proliferation and differentiation of the bronchial stem cells. Methods The isolated human bronchial epithelium was injured by 5\|fluorouracil(5-FU). HDAC1 expression in bronchial epithelium during the process of regeneration was analyzed by immunohistochemistry and Western blotting. Results 1.At 0 hour after 5-FU treatment, the bronchial epithelium shed and the expression of HDAC1 was negative. During 3-6 hours after the removal of 5-FU,the bronchial rings were covered with flattened epithelial cells and a few of the epithelial cells were HDAC1 positive. At the 12th hour, the epithelial cells were cuboidal and merged into pieces. The number of the HDAC1 positive cells increased obviously and a few HDAC1 positive cells were around one negative cell. At the 24th hour, the epithelial cells continued to increase and became stratified,HDAC1 was in top expression throughout all layers at this time; At the 48th hour, the epithelium restored similar to the pseudostratified structure,HDAC1 decreased slightly; The expression of HDAC1 was negative in normal bronchial epithelium. 2.Western blotting analysis showed that there were different HDAC1 levels at different times after the removal of 5-FU which in accordance with the change of immunohistochemistry. Conclusion With more differentiating cells appeared, more HDAC1 positive cells were observed, suggesting that HDAC1 can function as a molecular switch of bronchial epithelial stem cells fom proliferation to differentiation./P>
Key words: Bronchial stem cell, 5-Fluorouracil, Histone deacetylase 1, Molecular switch, Immunohistochemistry, Western blotting, Human
CLC Number:
R36
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: https://jpxb.bjmu.edu.cn/EN/10.3969/j.issn.0529-1356.2011.05.019
https://jpxb.bjmu.edu.cn/EN/Y2011/V42/I5/666
Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia
Analysis of cerebral gray matter structure in multiple sclerosis and neuromyelitis optica
Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice
Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease
Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage
Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle
Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats