Mortalin promotes ovarian cancer cell growth through MAPK-ERK signal pathway

doi:10.3969/j.issn.0529-1356.2014.03.008

Abstract

Abstract:

Objective By constructing mortalin stably expressing ovarian cancer cell lines in A2780 and A2780/cis, we demonstrate the role of mortalin in the ovarian cancer cell growth. Methods CCK-8 assay was used to measure cell viability in the overexpression mortalin group compared with the control group. The flow cytometry analysis was used to understand the effect of upregulated mortalin on the ovarian cancer cell cycle. Western blotting was used to determine the expression and phosphorylation level of MAPK/ERK and JNK/SAPK signal pathways. Results The results showed that increased expression of mortalin could accelerate ovarian cancer cell proliferation and promote G₁ transition, leading to a faster restoration of normal distribution of cell cycle. We found that mortalin overexpression significantly activated p-Raf and p-ERK1/2, but not p-JNK. Conclusion The results demonstrate that mortalin effect on the ovarian cancer cell proliferation contributes to active the MAPK-ERK signaling pathway.

Key words: Mortalin, Ovarian cancer, Cell cycle, MAPK-ERK signaling pathway, Plasmid construction, CCK-8, Western blotting, Human

HU Ying-ying HAN Yan-yan ZHAO Jia-wei YANG Ling LIU Wen ZUO Ji*. Mortalin promotes ovarian cancer cell growth through MAPK-ERK signal pathway[J]. AAS, 2014, 45(3): 338-343.

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